2014
DOI: 10.1007/s11010-014-2276-z
|View full text |Cite
|
Sign up to set email alerts
|

Endothelial progenitor cells proliferated via MEK-dependent p42 MAPK signaling pathway

Abstract: Endothelial progenitor cells (EPCs) clinical applications have been well reported. However, due to low number of EPCs that could be isolated, EPCs expansion study became one of the main focuses. Some optimized mediums to culture EPCs were currently available. However, the proliferation signaling pathway is not clearly disclosed yet. Peripheral blood was collected from eight healthy subjects, followed by mononuclear cells (MNCs) isolation. MNCs were then prepared and cultured for 2 days. After that, non-adheren… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
16
0

Year Published

2017
2017
2020
2020

Publication Types

Select...
8
1

Relationship

3
6

Authors

Journals

citations
Cited by 19 publications
(16 citation statements)
references
References 19 publications
0
16
0
Order By: Relevance
“…DAPI staining was performed as described by Sandra, et al (14,15) Cells were seeded onto coverslips, stimulated, rinsed in phosphate buffered saline (PBS) and fixed with icecold tricholoroacetic acid and gradient ethanol. Then cells were permeabilized with 0.1% BSA and 0.1% Triton X-100 for 3 minutes, and stained with 1 μg/ml 4',6-Diamidino-2-phenylindole (Calbiochem) for 3 minutes.…”
Section: '6-diamidino-2-phenylindole (Dapi) Stainingmentioning
confidence: 99%
“…DAPI staining was performed as described by Sandra, et al (14,15) Cells were seeded onto coverslips, stimulated, rinsed in phosphate buffered saline (PBS) and fixed with icecold tricholoroacetic acid and gradient ethanol. Then cells were permeabilized with 0.1% BSA and 0.1% Triton X-100 for 3 minutes, and stained with 1 μg/ml 4',6-Diamidino-2-phenylindole (Calbiochem) for 3 minutes.…”
Section: '6-diamidino-2-phenylindole (Dapi) Stainingmentioning
confidence: 99%
“…Peripheral blood mononuclear cells (PBMCs) were isolated from the blood sample by Ficoll Histopaque 1077 (Sigma-Aldrich, St. Louis, MO, USA). To isolate EPCs from PBMCs, a standard protocol was conducted as described previously [22]. Briefly, 5 × 10 5 cells/mL PBMCs were cultured in the fibronectin-coated 6-well plate with basal stemline II hematopoietic stem cell expansion medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 15% fetal bovine serum and 40 ng/mL vascular endothelial growth factor.…”
Section: Epcs Isolation and Culturementioning
confidence: 99%
“…13 The application of stem cell therapy in the medical field are rapidly developing. Efforts to achieve the applications also vary, such as the development of stem cell and its microenvironment isolation methods 14,15 , development of methods to increase the number of stem cells [16][17][18][19][20][21][22] , the development of stem cell differentiation methods 23 (including cell reprogramming) 24,25 , stem cell storage methods 26,27 , the development of stem cell transplantation methods 16,28 , etc. Since then, some particular stem cell types with their potential differentiation capacities can be isolated and cultured to meet the requirement of each clinical study to be explored.…”
Section: B I O P H a R M A C E U T I C A L I N S T I T U T Ementioning
confidence: 99%
“…Meanwhile, in some circumstances, cell number is not enough, so therefore cell number should be enriched in an in vitro stem cell culture setting. 16,17,[19][20][21][22] In an in vitro stem cell culture, besides suitable and sterile equipment, reagents such as culture medium, serum and antibiotics are all important. Although all those criteria are fulfilled, somehow stem cell enrichment cannot be achieved, cell number is still below the target.…”
Section: B I O P H a R M A C E U T I C A L I N S T I T U T Ementioning
confidence: 99%