Endothelial Progenitor Cells

Hristov, Mihail; Erl, Wolfgang; Weber, Peter

doi:10.1161/01.atv.0000073832.49290.b5

Cited by 685 publications

(315 citation statements)

References 51 publications

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“…In the present study, outgrowth cells were generated from majority of the early moyamoya patients, but rarely from control subjects or from advanced stage moyamoya patients. Although the exact cellular and/or paracrine mechanisms involved in EPC-mobilization, recruitment, and homing are unclear, this regenerative process appears to be regulated by a variety of chemokines and cytokines (Hristov et al, 2003). In terms of growth factors and cytokines in MMD, the vascular endothelial growth factor, fibroblast growth factor, hepatocyte growth factor, and plateletderived growth factor have been reported to increase in the cerebrospinal fluid and dural tissue in MMD patients (Hoshimaru et al, 1991;Aoyagi et al, 1996;Yoshimoto et al, 1996;Soriano et al, 2002;Kim et al, 2003;Nanba et al, 2004;Sakamoto et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Circulating Endothelial Progenitor Cells as a Pathogenetic Marker of Moyamoya Disease

Jung

Chu

Park

et al. 2008

J Cereb Blood Flow Metab

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Moyamoya disease (MMD) is an unusual form of chronic cerebrovascular occlusive disease that involves the formation of characteristically abnormal vessels. Recent studies have reported that colony-forming unit (CFU) and outgrowth cells represent a subpopulation of endothelial progenitor cells (EPCs). Here, we attempted to determine the significance of CFU number and outgrowth cell yield in MMD. Endothelial progenitor cells were isolated from the blood of 24 adult MMD patients and from 48 age-and risk factor-matched control subjects. After 7 days of culture, CFUs were determined, and yields of outgrowth cells were measured during 2 months of culture. The EPC function was also evaluated using matrigel plate assays. It was found that CFU numbers were significantly lower in MMD patients than in controls. Moreover, during long-term culture, outgrowth cells were isolated from only 10% of control subjects but from 33% of MMD patients, and CFU numbers and tube formation were found to be lower in advanced MMD cases than in those with early stage disease, whereas outgrowth cells were more frequently detected in those with early MMD and moyamoya vessels than in those with advanced disease. These characteristics of circulating EPCs reflect mixed conditions of vascular occlusion and abnormal vasculogenesis during the pathogenesis of MMD.

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Section: Discussionmentioning

confidence: 99%

Circulating Endothelial Progenitor Cells as a Pathogenetic Marker of Moyamoya Disease

Jung

Chu

Park

et al. 2008

J Cereb Blood Flow Metab

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“…So we would rather select EPCs derived from rat marrow as our subject in the following study. Bone marrow-derived EPC played critical role in postnatal vasculogenesis through pivotal bioactivities, mobilization, homing, migration, differentiation, and proliferation in angiovasculogenic tissues [5] . …”

Section: Introductionmentioning

confidence: 99%

The characteristics of bone marrow-derived endothelial progenitor cells and their effect on glioma

et al. 2012

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BackgroundEPCs were isolated primarily in 1997 by Asahara et al. and recent studies indicated that bone-marrow-derived EPCs contributed little to the endothelium of tumor vessels. Tumors of the CNS system demonstrate various features of angiogenesis.MethodsEPCs derived from rat bone marrow were isolated and cultured in M199 medium without any induced factors. EPCs were studied using immunohistochemical staining, Flow cytometry and culture under three-dimensional condition to determine EPCs’ characteristics in vitro. We also established an animal model by injecting EPCs marked with Hoechst 33342 into the back of BALB/c nude mice and performed hematoxylin-eosin (HE) and immunofluorescent staining to study EPCs’ features in vivo. To research effect of EPCs on glioma, animals bearing tumors model with C6 glioma were established. About 27 day after injection, we performed immunohistochemical staining and Immunofluorescence staining.ResultsOur results showed that EPCs derived from rat bone marrow appeared typical morphological characteristics and were positive of CD34, CD133, KDR and CD31 antigens at different time in vitro under the special M199 medium without any induced factors. The percentage of cells that expressed CD133 decreased gradually. In brief, the present study showed that EPCs derived from rat bone marrow differentiated into ECs in medium the without any induced factors and formed tubular structures in three-dimensional circumstances. Animal experiments suggested that EPCs differentiated into ECs and other else non-endothelial cells, and that EPCs contributed M199 of glioma.DiscussionThese findings provides some novel results about biological characteristics of EPCs in vivo and ex vivo, and an update on the effect of EPCs on glioma and which would be helpful for the overall understanding of EPCs and make EPCs to be implied on the clinical therapy.

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“…Circulating EPCs consist of cells at various stages of maturation. The CD133+ VEGFR-2+ cells are believed to be less mature or early circulating EPCs [17], while more mature circulating EPCs, which have lost CD133, are positive for CD34+ VEGFR-2+ and CD34+VE-cadherin+ [18].…”

Section: Introductionmentioning

confidence: 99%

Circulating endothelial progenitor cells, endothelial function, carotid intima–media thickness and circulating markers of endothelial dysfunction in people with type 1 diabetes without macrovascular disease or microalbuminuria

et al. 2009

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Aims/hypothesis Type 1 diabetes is associated with premature arterial disease. Bone-marrow derived, circulating endothelial progenitor cells (EPCs) are believed to contribute to endothelial repair. The hypothesis tested was that circulating EPCs are reduced in young people with type 1 diabetes without vascular injury and that this is associated with impaired endothelial function and increased carotid intima-media thickness (CIMT). Methods We compared 74 people with type 1 diabetes with 80 healthy controls. CD34, CD133, vascular endothelial (VE) growth factor receptor-2 (VEGFR-2) and VE-cadherin antibodies were used to quantify EPCs and progenitor cell subtypes using flow-cytometry. Ultrasound assessment of endothelial function by brachial artery flow-mediated dilatation (FMD) and CIMT was made. Circulating endothelial markers, inflammatory markers and plasma plasminogen activator inhibitor-1 (PAI-1) levels were measured. Results CD34+VE-cadherin+, CD133+VE-cadherin+ and CD133+VEGFR-2+ EPC counts were significantly lower in people with diabetes (46-69%; p=0.004-0.043). In people with type 1 diabetes, FMD was reduced by 45% (p<0.001) and CIMT increased by 25% (p<0.001), these being correlated (r=−0.25, p=0.033). There was a significant relationship between FMD and CD34+VE-cadherin+ (r = 0.39, p = 0.001), CD133+VEGFR-2+ (r =0.25, p = 0.037) and CD34+ (r=0.34, p=0.003) counts. Circulating high-sensitivity C-reactive protein, PAI-1, interleukin-6 and E-selectin were significantly higher in the diabetes group (p < 0.001 to p = 0.049), the last two of these correlating with FMD (r=−0.27, p=0.028 and r=−0.24, p=0.048, respectively). Conclusions/interpretation These findings suggest that abnormalities of endothelial function in addition to proinflammatory and pro-thrombotic states are already common in people with type 1 diabetes before development of clinically evident arterial damage. Low EPC counts confirm risk of macrovascular complications and may account for impaired endothelial function and predict future cardiovascular events.

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Endothelial Progenitor Cells

Abstract: Abstract-Postnatal

Cited by 685 publications

References 51 publications

Circulating Endothelial Progenitor Cells as a Pathogenetic Marker of Moyamoya Disease

Circulating Endothelial Progenitor Cells as a Pathogenetic Marker of Moyamoya Disease

The characteristics of bone marrow-derived endothelial progenitor cells and their effect on glioma

Circulating endothelial progenitor cells, endothelial function, carotid intima–media thickness and circulating markers of endothelial dysfunction in people with type 1 diabetes without macrovascular disease or microalbuminuria

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