Endothelial nitric oxide synthase gene polymorphisms -786T >C and 894G >T in coronary artery bypass graft surgery patients

Abstract

Polymorphisms in the endothelial nitric oxide synthase (eNOS) gene (-786T >C and 894G >T) enhance endo-thelial dysfunction and have been studied in relation to coronary artery disease (CAD). In the present study, we examined the association of the above polymorphisms with CAD, as well as with myocardial infarction (MI), hypertension, diabetes and smoking in CAD patients. Study subjects consisted of 154 consecutive coronary artery bypass graft (CABG) patients and 155 non-CAD controls. eNOS -786T >C and 894G >T polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism. The estimated frequencies of the -786C and 894T alleles did not differ between the two groups (p = 0.46 and p = 0.84, respectively). The prevalence of eNOS polymorphisms was not associated with MI, hypertension or diabetes in CABG patients; however, we found that the 894TT genotype and 894T allele were significantly more frequent in current/past smoker CABG patients (16.7 per cent and 39.6 per cent, respectively) compared with never smoker CABG patients (6.1 per cent and 24.4 per cent, respectively) (p = 0.01 and p < 0.01, respectively). We found no association of eNOS -786C and 894T variant alleles with CAD; however, within CABG patients, a gene-environment interaction was found between the eNOS 894T allele and smoking.

Randomized trial of inhaled nitric oxide to treat acute pulmonary embolism: The iNOPE trial

“…Two common polymorphisms(- 786T>C and 894G>T) will be assessed with a 5′-allele discrimination PCR assay using primer sequences previously described by our group and others. 19,20 Both polymorphisms impair eNOS synthesis of endogenous NO, impair endothelial-induced vascular smooth muscle relaxation, increase red cell fragility and increase the risk of PE in Caucasians and Asians. 20-22 The hypothesis is that patients with these variations will have improved response to inhaled NO.…”

Endothelial nitric oxide synthase polymorphism and prognosis in systolic heart failure patients

“…Several eNOS genomic polymorphisms have been described in association with pathological cardiovascular conditions; among them is the exon 7 G894T eNOS polymorphism, leading to amino acid substitution at position 298 (conversion of glutamate to aspartate) [6]. The clinical significance of the G894T eNOS polymorphism in humans was assessed in multiple studies, and significant association was found between this eNOS polymorphism and various cardiovascular conditions, including vasospastic angina, hypertension, and carotid and coronary atherosclerosis [7][8][9][10][11][12][13][14]. Several recent meta-analyses concluded that eNOS G894T polymorphism may increase the risk of developing thrombotic diseases and may play an important role in the development of hypertension, ischemic stroke and coronary heart disease [15][16][17][18][19].…”

Research Article Atherosclerosis: analysis of the eNOS (T786C) gene polymorphism.

“…This result is similar to that of Piccoli et al (2012), where they analyzed a South-Brazilian population and found a greater prevalence of the TC genotype in patients with acute coronary syndrome (ACS) and controls. Ragia et al (2010) found that the TC genotype (55.2% in the case group and 54.8% in control) had a higher prevalence concerning the other genotypes and they also did not find a statistically significant difference for patients who underwent myocardial revascularization surgery. A study conducted by Ghilardi et al (2002) in Italian patients found that the genotypic distribution in the controls was 54 TT (41%), 61 CT (46%), and 18 CC (13%), corroborating the studies in the present research.…”