Endothelial integrity after venous cryopreservation

Sachs, Stephen M.; Ricotta, John J.; Scott, Daniel E.; DeWeese, James A.

doi:10.1016/0022-4804(82)90094-4

Cited by 26 publications

(8 citation statements)

References 13 publications

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“…29 Similar results have also been reported using cryopreserved frozen autologous veins in canine models. 30,31 Unlike allografts, the extensive endothelial denudation does not usually occur in fresh autografts. 13 The present quantitative analyses of fresh, control vein grafts agrees with findings in fresh autologous vein grafts in the literature.…”

Section: Discussionmentioning

confidence: 97%

Quantitative Analyses of Vitrified Autologous Venous Arterial Bypass Graft Explants

Brockbank

Song

Greene

et al. 2007

Cell Preservation Technology

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The purpose of this study was to compare vitrified small diameter venous arterial bypass graft explants with fresh graft controls using quantitative morphometrics and smooth muscle physiology. Fresh and vitrified reversed ipsilateral external jugular veins were used as autologous common carotid interposition bypass grafts on the right side in New Zealand White rabbits. Animals were sacrificed at either 2 or 4 weeks after implantation, and the vein grafts were harvested for study. Histology revealed that the vitrified grafts initially arteriolized more slowly than untreated grafts and that they caught up by 4 weeks postimplantation. Histomorphometrics revealed that the intimal area was less in vitrified grafts at 2 weeks (p ‫؍‬ 0.007) and that the luminal area was less in vitrified grafts at 4 weeks (p ‫؍‬ 0.002). All other morphometric parameters were not significantly different. Smooth muscle physiology demonstrated a tendency for the responses of vitrified grafts to be lower than controls, not statistically significantly different to controls for three of the four contractile agonists at each time point or for relaxation induced by acetycholine. Sodium nitroprusside-induced relaxations appeared to be identical. Sensitivity to histamine was decreased at 2 weeks, and the sensitivity to norepinephrine was increased at 4 weeks in vitrified explants (Ͻ0.05). This study demonstrates that vitrification slows down the arterialization process; however, by the end of the month after implantation the vein grafts have similar smooth muscle functions and histology. The greatest concern regarding use of this method for preservation of blood vessels for clinical application was the statistically significant decrease in luminal area observed at 1 month. Longer term in vivo studies are required to determine whether or not the observed luminal decrease progresses to occlusion.

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Section: Discussionmentioning

confidence: 97%

Quantitative Analyses of Vitrified Autologous Venous Arterial Bypass Graft Explants

Brockbank

Song

Greene

et al. 2007

Cell Preservation Technology

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“…[5][6][7][8][9][10] The introduction of rate-controlled freezing and the cryopreservant DMSO has resulted in the preservation of up to 80% of the vascular endothelium during the cryopreservation process. 1,2 These DMSO-cryopreserved femoral veins have been indicated for use in liver transplantation as conduits to attach the hepatic artery or portal vein, as venous valve replacements in patients with chronic venous insufficiency, and as conduits for arteriovenous fistulas when suitable autogenous material is not available. 3 Although the presence of viable vascular endothelium has significantly increased graft patency rates in animal models, it raises concerns about the antigenicity of the vein allograft and the potential immune response of the recipient.…”

Section: Discussionmentioning

confidence: 99%

“…DMSOcryopreserved cadaveric veins offer the potential advantage of decreased infection and thrombosis because up to 80% of the vascular endothelial cells lining the graft have been demonstrated to be viable and biologically active at time of engraftment. 1,2 This advantage, however, may lead to allosensitization in nonimmunosuppressed patients exposed to allogenic class I and II major histocompatability complex (MHC) antigens present on viable endothelial cells. The purpose of this study was to examine the impact 139…”

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confidence: 99%

Use of cryopreserved cadaveric vein allograft for hemodialysis access precludes kidney transplantation because of allosensitization

Benedetto

Lipkowitz²,

Madden³

et al. 2001

Journal of Vascular Surgery

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“…13 Studies have also been conducted in which viable endothelium has been demonstrated, following cryopreservation of large blood vessels. 14 In our study, we used factor VIII staining to establish the functional status of the cryopreserved endothelium, which we found to be positive in cryopreserved veins. It is possible that the observed amount of viable endothelium may have been helpful in maintaining patency up to 21 days.…”

Section: Cryomentioning

confidence: 94%

The Use of Cryopreserved Venous Allografts in Microvascular Surgery without Immunosuppression: An Experimental Study

Narayanan

Ahn²,

Monstrey³

et al. 1993

J reconstr Microsurg

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Excellent patency rates are currently established in the use of autogenous veins as interposition vein grafts in microsurgical practice. Allografts may be a viable alternative source of vein grafts. Recent advances in cryobiology have enabled the controlled freezing of tissues with preservation of vital cellular elements. Although several reports have shown the successful use of cryopreserved large-vessel allografts, few have addressed cryopreservation of microvessels (1 to 2 mm in diameter). In this study, the authors have successfully cryopreserved femoral veins in a rat model and transplanted them as interposition vein grafts into arterial defects across major histocompatibility barriers (ACI to Lewis). Short-term patencies (21 days) were determined, and histologic and scanning electron microscopic analysis were performed. Patency of 100 percent was achieved in both fresh control veins and in cryopreserved veins at 21 days.

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Endothelial integrity after venous cryopreservation

Cited by 26 publications

References 13 publications

Quantitative Analyses of Vitrified Autologous Venous Arterial Bypass Graft Explants

Quantitative Analyses of Vitrified Autologous Venous Arterial Bypass Graft Explants

Use of cryopreserved cadaveric vein allograft for hemodialysis access precludes kidney transplantation because of allosensitization

The Use of Cryopreserved Venous Allografts in Microvascular Surgery without Immunosuppression: An Experimental Study

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