Use of cryopreserved cadaveric vein allograft for hemodialysis access precludes kidney transplantation because of allosensitization

Benedetto, Bernard; Lipkowitz, George S.; Madden, Robert L.; Kurbanov, Alexander; Hull, David; Miller, Maureen; Bow, Laurine

doi:10.1067/mva.2001.114206

Cited by 36 publications

(30 citation statements)

References 20 publications

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“…Second, exposure to alloantigens results in the production of antibodies that limit a patient's ability to receive solid organ transplants. Use of cryopreserved vein as a conduit for hemodialysis access has been associated with increased panel reactive antigens [13]; decellularization of these conduits prevents this important complication [14]. This study demonstrates that decellularization limits the immunological response to vein allograft as evidenced by decreased mononuclear cell infiltrate at 2 weeks and limited intimal hyperplasia at 8 weeks.…”

Section: Discussionmentioning

confidence: 86%

In Vivo Behavior of Decellularized Vein Allograft1,2

Martin

Schaner

Tulenko

et al. 2005

Journal of Surgical Research

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Section: Discussionmentioning

confidence: 86%

In Vivo Behavior of Decellularized Vein Allograft1,2

Martin

Schaner

Tulenko

et al. 2005

Journal of Surgical Research

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“…Decellularised aortic valves present a promising alternative for aortic valve replacement in selected patients. For aortic grafts, cryopreserved aortas have been used in the clinic with reports of good long-term survival and patency (Gomez-Caro et al 2008 ; Noel et al 2002 ), but the presence of remaining donor cells/material in cryopreserved allografts may however lead to allosensitization (Benedetto et al 2001 ; Wilshaw et al 2012 ).…”

Section: Discussionmentioning

confidence: 99%

“…The use of vascular allografts may however lead to allosensitization (Benedetto et al 2001 ; Wilshaw et al 2012 ). Aortic allograft conduits (and heart valves) have been shown to undergo degenerative changes, including calcification, following implantation.…”

Section: Introductionmentioning

confidence: 99%

Development and characterisation of a large diameter decellularised vascular allograft

et al. 2017

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The aims of this study were to develop a biological large diameter vascular graft by decellularisation of native human aorta to remove the immunogenic cells whilst retaining the essential biomechanical, and biochemical properties for the ultimate benefit of patients with infected synthetic grafts. Donor aortas (n = 6) were subjected to an adaptation of a propriety decellularisation process to remove the cells and acellularity assessed by histological analysis and extraction and quantification of total DNA. The biocompatibility of the acellular aortas was determined using standard contact cytotoxicity tests. Collagen and denatured collagen content of aortas was determined and immunohistochemistry was used to determine the presence of specific extracellular matrix proteins. Donor aortas (n = 6) were divided into two, with one half subject to decellularisation and the other half retained as native tissue. The native and decellularised aorta sections were then subject to uniaxial tensile testing to failure [axial and circumferential directions] and suture retention testing. The data was compared using a paired t-test. Histological evaluation showed an absence of cells in the treated aortas and retention of histoarchitecture including elastin content. The decellularised aortas had less than 15 ng mg−1 total DNA per dry weight (mean 94% reduction) and were biocompatible as determined by in vitro contact cytotoxicity tests. There were no gross changes in the histoarchitecture [elastin and collagen matrix] of the acellular aortas compared to native controls. The decellularisation process also reduced calcium deposits within the tissue. The uniaxial tensile and suture retention testing revealed no significant differences in the material properties (p > 0.05) of decellularised aorta. The decellularisation procedure resulted in minimal changes to the biological and biomechanical properties of the donor aortas. Acellular donor aorta has excellent potential for use as a large diameter vascular graft.

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“…hemodialysis access, infrainguinal bypass or portal vein reconstruction as part of the surgical treatment of hilar cholangiocarcinoma. [32][33][34][35][36][37] The use of AG for reconstruction during pancreatic resection has only been described specifically in two small case series. 24,25 When a tension-free primary end-to-end anastomosis cannot be achieved, the use of an interposition graft can contribute to the completion of the resection.…”

Section: Discussionmentioning

confidence: 99%

Cold-stored cadaveric venous allograft for superior mesenteric/portal vein reconstruction during pancreatic surgery

Kleive

Berstad

Verbeke

et al. 2016

HPB

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Use of cryopreserved cadaveric vein allograft for hemodialysis access precludes kidney transplantation because of allosensitization

Abstract: The use of dimethyl sulfoxide-cryopreserved cadaveric vein allografts for hemodialysis access leads to broad allosensitization as measured by PRA assay. Cryopreserved cadaveric vein allografts should not be used for hemodialysis access in potential kidney transplant recipients.

Cited by 36 publications

References 20 publications

In Vivo Behavior of Decellularized Vein Allograft1,2

In Vivo Behavior of Decellularized Vein Allograft1,2

Development and characterisation of a large diameter decellularised vascular allograft

Cold-stored cadaveric venous allograft for superior mesenteric/portal vein reconstruction during pancreatic surgery

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