Endothelial directed collective migration depends on substrate stiffness via localized myosin contractility and cell-matrix interactions

Canver, Adam C.; Ngo, Olivia; Urbano, Rebecca Lownes; Clyne, Alisa Morss

doi:10.1016/j.jbiomech.2015.12.037

Cited by 33 publications

(36 citation statements)

References 43 publications

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“…Canver et al reported that the migration speed of a confluent monolayer of endothelial cells on collagen gel falls between 300 and 700 μ m for the first 24 hours, depending on the substrate stiffness [25]. In our study, cells migrated approximately 250 μ m in the same period.…”

Section: Discussionsupporting

confidence: 64%

Patterning of Endothelial Cells and Mesenchymal Stem Cells by Laser-Assisted Bioprinting to Study Cell Migration

Bourget

Kérourédan

Medina

et al. 2016

BioMed Research International

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Tissue engineering of large organs is currently limited by the lack of potent vascularization in vitro. Tissue-engineered bone grafts can be prevascularized in vitro using endothelial cells (ECs). The microvascular network architecture could be controlled by printing ECs following a specific pattern. Using laser-assisted bioprinting, we investigated the effect of distance between printed cell islets and the influence of coprinted mesenchymal cells on migration. When printed alone, ECs spread out evenly on the collagen hydrogel, regardless of the distance between cell islets. However, when printed in coculture with mesenchymal cells by laser-assisted bioprinting, they remained in the printed area. Therefore, the presence of mesenchymal cell is mandatory in order to create a pattern that will be conserved over time. This work describes an interesting approach to study cell migration that could be reproduced to study the effect of trophic factors.

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Section: Discussionsupporting

confidence: 64%

Patterning of Endothelial Cells and Mesenchymal Stem Cells by Laser-Assisted Bioprinting to Study Cell Migration

Bourget

Kérourédan

Medina

et al. 2016

BioMed Research International

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“…Finally, knockdown of the α5β1 integrin reduced migration for ECs on 14 and 50 kPa gels and eliminated differences in migration distance between the 3 stiffness conditions tested. [86] …”

Section: Mechanical Stimuli In the Vasculaturementioning

confidence: 99%

Endothelial Cell Mechanotransduction in the Dynamic Vascular Environment

2018

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The vascular endothelial cells (ECs) that line the inner layer of blood vessels are responsible for maintaining vascular homeostasis under physiological conditions. In the presence of disease or injury, ECs can become dysfunctional and contribute to a progressive decline in vascular health. ECs are constantly exposed to a variety of dynamic mechanical stimuli, including hemodynamic shear stress, pulsatile stretch, and passive signaling cues derived from the extracellular matrix. This review describes the molecular mechanisms by which ECs perceive and interpret these mechanical signals. The translational applications of mechanosensing are then discussed in the context of endothelial-to-mesenchymal transition and engineering of vascular grafts.

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“…[4][5][6][7][8] Pelham and Wang first reported that substrate stiffness could affect the motility and spread of fibroblasts and kidney epithelial cells. 9,10 Since then, there have been increasingly widespread and profound studies on substrate stiffness and cell interactions, such as adhesion, 11 migration, 12,13 cell differentiation and proliferation. 14,15 It is also involved in cellular processes such as angiogenesis, 16 tumor metastasis, 13,17 embryonic development and wound healing.…”

Section: Introductionmentioning

confidence: 99%

A review of gradient stiffness hydrogels used in tissue engineering and regenerative medicine

Xia

Liu

Yang

2017

J Biomedical Materials Res

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Substrate stiffness is known to impact characteristics including cell differentiation, proliferation, migration and apoptosis. Hydrogels are polymeric materials distinguished by high water content and diverse physical properties. Gradient stiffness hydrogels are designed by the need to develop biologically friendly materials as extracellular matrix (ECM) alternatives to replace the separated and narrow-ranged hydrogel substrates. Important new discoveries in cell behaviors have been realized with model gradient stiffness hydrogel systems from the two-dimensional (2D) to three-dimensional (3D) scale. Basic and clinical applications for gradient stiffness hydrogels in tissue engineering and regenerative medicine continue to drive the development of stiffness and structure varied hydrogels. Given the importance of gradient stiffness hydrogels in basic research and biomedical applications, there is a clear need for systems for gradient stiffness hydrogel design strategies and their applications. This review will highlight past work in the field of gradient stiffness hydrogels fabrication methods, mechanical property test, applications as well as areas for future study. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1799-1812, 2017.

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Endothelial directed collective migration depends on substrate stiffness via localized myosin contractility and cell-matrix interactions

Cited by 33 publications

References 43 publications

Patterning of Endothelial Cells and Mesenchymal Stem Cells by Laser-Assisted Bioprinting to Study Cell Migration

Patterning of Endothelial Cells and Mesenchymal Stem Cells by Laser-Assisted Bioprinting to Study Cell Migration

Endothelial Cell Mechanotransduction in the Dynamic Vascular Environment

A review of gradient stiffness hydrogels used in tissue engineering and regenerative medicine

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