Endothelial cell viability in the rat aortic wall

Christy, Jeffrey P.; Lupinetti, Flavian M.; Mardan, Ali H.; Thompson, Sandra A.

doi:10.1016/0003-4975(91)90784-n

Cited by 12 publications

(8 citation statements)

References 24 publications

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Section: Discussionmentioning

confidence: 98%

“…7,10 This lectin has previously been reported to exhibit affinity for, and establish the presence of, both macrovascular and microvascular endothelial cells in rat tissue. 10,11 This lectin will also recognize certain classes of activated monocytes and macrophages. 10 Reaction of histologic sections prepared from implants in our study resulted in clear identification of endothelial cell profiles, especially endothelium present in tube structures of varying caliber.…”

Section: Discussionmentioning

confidence: 98%

“…10,11 This lectin will also recognize certain classes of activated monocytes and macrophages. 10 Reaction of histologic sections prepared from implants in our study resulted in clear identification of endothelial cell profiles, especially endothelium present in tube structures of varying caliber. Again a striking difference was observed in the distribution of microvascular endothelial cell profiles observed in fat versus subcutaneous tissue implants.…”

Section: Discussionmentioning

confidence: 99%

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Differential healing and neovascularization of ePTFE implants in subcutaneous versus adipose tissue

Williams

Berman

Kleinert

1997

J. Biomed. Mater. Res.

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The preclinical evaluation of polymer biocompatibility is often performed using animal subcutaneous implant models. The choice of subcutaneous tissue as the implant site is due to a number of factors including simplicity of the surgery involved. Results from subcutaneous implants cannot necessarily be extrapolated to other tissues due to the differences in cellular composition of tissues. We have evaluated and compared the healing characteristics of expanded polytetrafluoroethylene (ePTFE) discs implanted in either subcutaneous tissue or epididymal fat pad tissue in rats. Following 3 and 5 weeks of implantation, the healing characteristics of discs were evaluated histologically with particular emphasis on tissue and polymer neovascularization. Implants placed in subcutaneous tissue exhibited limited formation of new microvascular elements within and directly in contact with the polymer, and the formation of an extensive fibrous capsule. In contrast, ePTFE implanted in the epididymal fat pads of rats exhibited extensive neovascularization of tissue surrounding the polymer, penetration of these microvascular cells into the graft interstices for distances < or = 100 microns and no morphological evidence of a fibrous capsule. The rat epididymal fat pad provides an alternative tissue for polymer healing evaluations. Due to the extensive presence of fat in subcutaneous tissue in humans, we suggest the fat pad model provides a more relevant preclinical evaluation of the healing characteristics of polymers used clinically in anatomic positions which contain significant amounts of fat.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Differential healing and neovascularization of ePTFE implants in subcutaneous versus adipose tissue

Williams

Berman

Kleinert

1997

J. Biomed. Mater. Res.

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“…The whole cell‐pellet was resuspended by PBS. Then, 0.004% GS1‐FITC (ICN Biomedicals Inc., Aurora, OH, U.S.A.) mixed with 2 m M CaCl 2 and 2 m M MgCl 2 , and 20 μm PI (Sigma) was added to the dissociated cell suspended PBS solution (2). The GS1‐FITC/PI double stained cells were detected by an FCM (FACSCalibur, Becton Dickinson, San Jose, CA, U.S.A.), and unstained dissociated cell suspension was regarded as the negative control.…”

Section: Methodsmentioning

confidence: 99%

“…Recent studies suggested that certain cells in a given species exhibit distinct cell surface glycosylation properties differing from the other cells, and EC appear to express certain α‐D‐galactosyl residue. It was reported that a tetrameric Griffonia simplicifolia agglutins (GS1) shows prominent binding only to the α‐D‐galactosyl residue of blood vessel EC (2). Fluorescein isothiocyanate (FITC) conjugated with (GS1) staining differentiates EC from the other cells in flow cytometry (FCM).…”

mentioning

confidence: 99%

Specific Determination of Endothelial Cell Viability in the Whole Cell Fraction from Cryopreserved Canine Femoral Veins Using Flow Cytometry

Park¹,

Sung²,

Lee³

et al. 2000

Artificial Organs

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An efficient method for specifically determining the viability of endothelial cells (EC) from cells dissociated from the human saphenous vein was investigated. Three different methods, trypan blue staining assay, [3H]-proline incorporation assay, and flow cytometry (FCM), combined with the fluorescein isothiocyanate conjugated with Griffonia simplicifolia agglutins (GS1-FITC)/propidium iodide (PI) double staining, were used. Both trypan blue staining and [3H] proline incorporation assays demonstrated less sensitivity to determine viability of EC differentially from the other cells. FITC-GS1 showed prominent binding to the vascular EC and could be counted by FCM including PI on dead cells. Following the cryopreservation process, the GS1-FITC/PI FCM analytical method was adopted to test simultaneously the viability of whole cells and EC from the same tissue, human saphenous veins, and mongrel dogs' femoral veins after harvesting, antibiotic solution treatment, and thawing. The viability of the whole cells from veins decreased with a significant difference (p < 0.05) from that of EC after thawing.

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Calcification of valved aortic allografts in rats: Effects of age, crosslinking, and inhibitors

Levy

Underwood

et al. 1995

J. Biomed. Mater. Res.

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Experiments were carried out to investigate rat aortic allograft calcification using valved abdominal aortic allografts. Results indicated that this was a potentially useful model for investigating fresh allograft calcification, as well as mineralization of glutaraldehyde-crosslinked valved allografts. Valve cusp results, however, were not comparable to those noted in large animal or human studies, while aortic wall calcification was more comparable. Calcification inhibitor investigations demonstrated that nearly complete inhibition of the calcification of the aortic wall of glutaraldehyde-crosslinked allografts was achieved using a number of individual inhibitors, including controlled release diphosphonates, and pretreatment with either ferric chloride or aluminum chloride. However, aminopropanehydroxydiphosphonate pretreatment was not efficacious, and sodium dodecyl sulfate pretreatment was only partially effective for inhibiting the aortic wall calcification in the glutaraldehyde-crosslinked allografts. It is concluded that valved aortic allografts in rats provide a useful model for investigating aortic wall (but not valve cusp) calcification and its inhibition.

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Endothelial cell viability in the rat aortic wall

Cited by 12 publications

References 24 publications

Differential healing and neovascularization of ePTFE implants in subcutaneous versus adipose tissue

Differential healing and neovascularization of ePTFE implants in subcutaneous versus adipose tissue

Specific Determination of Endothelial Cell Viability in the Whole Cell Fraction from Cryopreserved Canine Femoral Veins Using Flow Cytometry

Calcification of valved aortic allografts in rats: Effects of age, crosslinking, and inhibitors

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