Endothelial Cell-to-Cell Junctions: Adhesion and Signaling in Physiology and Pathology

Lampugnani, Maria Grazia

doi:10.1101/cshperspect.a006528

Cited by 60 publications

(50 citation statements)

References 86 publications

(104 reference statements)

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“…For example, they have less tight endothelial cell junctions [46], making these neovessels more permeable, and hence they may be more easily affected by ultrasound-activated microbubbles. However, neovessels are also associated with tumors and atherosclerotic plaques [47]- [49], which may make the caM model a relevant in vivo model for studying ultrasound and microbubble-mediated therapy to treat these diseases.…”

Section: Discussionmentioning

confidence: 99%

Targeted microbubble mediated sonoporation of endothelial cells in vivo

Skachkov

Luan

Steen

et al. 2014

IEEE Trans. Ultrason., Ferroelect., Freq. Contr.

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Ultrasound contrast agents as drug-delivery systems are an emerging field. Recently, we reported that targeted microbubbles are able to sonoporate endothelial cells in vitro. In this study, we investigated whether targeted microbubbles can also induce sonoporation of endothelial cells in vivo, thereby making it possible to combine molecular imaging and drug delivery. Live chicken embryos were chosen as the in vivo model. αvß3-targeted microbubbles attached to the vessel wall of the chicken embryo were insonified at 1 MHz at 150 kPa (1 × 10,000 cycles) and at 200 kPa (1 × 1000 cycles) peak negative acoustic pressure. Sonoporation was studied by intravital microscopy using the model drug propidium iodide (PI). Endothelial cell PI uptake was observed in 48% of microbubble-vessel-wall complexes at 150 kPa (n = 140) and in 33% at 200 kPa (n = 140). Efficiency of PI uptake depended on the local targeted microbubble concentration and increased up to 80% for clusters of 10 to 16 targeted microbubbles. Ultrasound or targeted microbubbles alone did not induce PI uptake. This intravital microscopy study reveals that sonoporation can be visualized and induced in vivo using targeted microbubbles.

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Section: Discussionmentioning

confidence: 99%

Targeted microbubble mediated sonoporation of endothelial cells in vivo

Skachkov

Luan

Steen

et al. 2014

IEEE Trans. Ultrason., Ferroelect., Freq. Contr.

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“…Once the tip cells anastomose or inosculate with other tip cells [23], vessel maturation takes place and this involves mural cell recruitment, extracellular matrix deposition, and the commencement of blood flow. A key feature of vasculogenesis and angiogenesis is central vascular lumen formation, the complexity of which has recently been reviewed [24][25][26][27]. The cord hollowing model of lumenization highlights the importance of interendothelial junctions and apicobasal polarity.…”

Section: Introductionmentioning

confidence: 99%

“…Multicellular endothelial cell cords form, migrate into the stroma, lose apicobasal polarity and cord junctions, and increase to two or three cell thicknesses. Subsequently, endothelial cell repulsion, junctional rearrangements, and change in shape of endothelial cells result in unicellular tube formation [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

The Hematopoietic Chemokine CXCL12 Promotes Integration of Human Endothelial Colony Forming Cell–Derived Cells into Immature Vessel Networks

Newey

Tsaknakis

Khoo

et al. 2014

Stem Cells and Development

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“…Endothelial TJ constitute ,20% of the total endothelial junctional complexes (Wójciak-Stothard et al 2001). Claudin-5 is one of the main TJ transmembrane proteins and can form homotypic or heterotypic binding (Lampugnani 2012). The peripheral membrane adaptor protein ZO-1 links the carboxyl terminal of transmembrane adhesion proteins with a-catenin, spectrin and F-actin.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of angiopoietin-1 (ANGPT1) affects vascular integrity in ovarian hyperstimulation syndrome (OHSS)

Scotti

Abramovich

Pascuali

et al. 2016

Reprod. Fertil. Dev.

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Abstract. Ovarian hyperstimulation syndrome (OHSS) is a complication of ovarian stimulation with gonadotrophins following human chorionic gonadotrophin (hCG) administration. The relationship between hCG and OHSS is partly mediated via the production of angiogenic factors, such as vascular endothelial growth factor A (VEGFA) and angiopoietins (ANGPTs). Here, we investigated the effect of ANGPT1 inhibition on ovarian angiogenesis in follicular fluid (FF) from women at risk of OHSS, using the chorioallantoic membrane (CAM) of quail embryos as an experimental model. We also analysed cytoskeletal changes and endothelial junction protein expression induced by this FF in the presence or absence of an ANGPT1-neutralising antibody in endothelial cell cultures. The presence of this antibody restored the number of vascular branch points and integrin a v b 3 levels in the CAMs to control values. ANGPT1 inhibition in FF from OHSS patients also restored the levels of claudin-5, vascular endothelial cadherin and phosphorylated b-catenin and partially reversed actin redistribution in endothelial cells. Our findings suggest that ANGPT1 increases pathophysiological angiogenesis in patients at risk of OHSS by acting on tight and adherens junction proteins. Elucidating the mechanisms by which ANGPT1 regulates vascular development and cell-cell junctions in OHSS will contribute to identifying new therapeutic targets for the treatment of human diseases with aberrant vascular leakage.

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Endothelial Cell-to-Cell Junctions: Adhesion and Signaling in Physiology and Pathology

Cited by 60 publications

References 86 publications

Targeted microbubble mediated sonoporation of endothelial cells in vivo

Targeted microbubble mediated sonoporation of endothelial cells in vivo

The Hematopoietic Chemokine CXCL12 Promotes Integration of Human Endothelial Colony Forming Cell–Derived Cells into Immature Vessel Networks

Inhibition of angiopoietin-1 (ANGPT1) affects vascular integrity in ovarian hyperstimulation syndrome (OHSS)

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