Endothelial Cell Migration on Poly(ε-caprolactone) Nanofibers Coated with a Nanohybrid Shish-Kebab Structure Mimicking Collagen Fibrils

Guo, Xin; Wang, Xiaofeng; Li, Xuyan; Jiang, Yongchao; Han, Shanshan; Ma, Lei; Guo, Huijun; Wang, Zhenxing; Li, Qian

doi:10.1021/acs.biomac.9b01638

Cited by 36 publications

(41 citation statements)

References 53 publications

(113 reference statements)

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“…For instance, in our previous study, a polycaprolactone (PCL) self-induced nanohybrid shish-kebab structure consisting of PCL nanofibers and spaced PCL crystal lamellae grown on the fibers was employed to regulate cell affinity and migration. 6 However, when considering that an engineering scaffold should mimic the micro-environment of extracellular matrix to the maximum extent, hydrogel scaffolds are preferred due to their inherent soft-tissue-like property, tunable modification, and three-dimensional intact mesh network, which are beneficial to cell culture and mass transfer. 7,8…”

Section: Introductionmentioning

confidence: 99%

Preparation of gelatin-based hydrogels with tunable mechanical properties and modulation on cell–matrix interactions

Jiang

Wang

et al. 2021

J Biomater Appl

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Natural polymer material-based hydrogels normally show inferior mechanical stability and strength to bear large deformation and cyclic loading, therefore their applications in food, biomedical and tissue engineering fields are greatly limited. In this study, gelatin-based hydrogels with remarkable stability, as well as tunable mechanical properties, were prepared via a facile method known as the Hofmeister effect. The higher concentration of potassium sulfatesolution resulted in more dehydration and molecular chain folding, thus the treated hydrogels showed significantly improved tensile and compressive modulus, and decreased equilibrium swelling ratio, as revealed by scanning electron microscopy (SEM), Fourier transform infraredspectroscopy (FTIR), and mechanical tests, etc. Additionally, the reinforced hydrogels were recoverable and biocompatible to modulate the proliferation behavior of human umbilical vein endothelial cells. In conclusion, this paper provides a facile reference for tuning mechanical properties of gelatin-based hydrogels and cell-hydrogel interactions, which show potential capacity in tissue engineering and biomedical fields.

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Section: Introductionmentioning

confidence: 99%

Preparation of gelatin-based hydrogels with tunable mechanical properties and modulation on cell–matrix interactions

Jiang

Wang

et al. 2021

J Biomater Appl

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“…But PTBSK still maintained a moderately hydrophilic surface, which was conducive to the growth of cells on the surface. [ 14 ] At the same time, the increase in hydrophobicity caused by the shish‐kebab structure also played a positive role in slowing down the release of E7‐BMP‐2.…”

Section: Resultsmentioning

confidence: 99%

Hierarchical Nanostructured Electrospun Membrane with Periosteum‐Mimic Microenvironment for Enhanced Bone Regeneration

Liu

Shang

et al. 2021

Adv Healthcare Materials

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An ideal periosteum substitute should be able to mimic the periosteum microenvironment that continuously provides growth factors, recruits osteoblasts, and subsequent extracellular matrix (ECM) mineralization to accelerate bone regeneration. Here, a calcium‐binding peptide‐loaded poly(ε‐caprolactone) (PCL) electrospun membrane modified by the shish‐kebab structure that can mimic the periosteum microenvironment was developed as a bionic periosteum. The calcium‐binding peptide formed by the negatively charged heptaglutamate domain (E7) in the E7‐BMP‐2 with calcium ion in the tricalcium phosphate sol (TCP sol) through electrostatic chelation not only extended the release cycle of E7‐BMP‐2 but also promoted the biomineralization of the bionic periosteum. Cell experiments showed that the bionic periosteum could significantly improve the osteogenic differentiation of the rat‐bone marrow‐derived mesenchymal stem cells (rBMSCs) through both chemical composition and physical structure. The in vivo evaluation of the bionic periosteum confirmed the inherent osteogenesis of this periosteum microenvironment, which could promote the regeneration of vascularized bone tissue. Therefore, the hierarchical nanostructured electrospun membrane with periosteum‐mimic microenvironment is a promising periosteum substitute for the treatment of bone defects.

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“…The fibrous samples of PCL-A and PCL-R were created by electrospinning with different tension forces by two different collectors, namely, rotating drum and flat plane, respectively. The PCL-S sample was obtained according to our previous work . The crystal lamellae are arranged periodically on the fiber, forming a more abundant surface structure.…”

Section: Resultsmentioning

confidence: 99%

Endothelial Cell Migration Regulated by Surface Topography of Poly(ε-caprolactone) Nanofibers

Zhang

Wang

Zhang

et al. 2021

ACS Biomater. Sci. Eng.

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The study of cell migration on biomaterials is of great significance in tissue engineering and regenerative medicine. In recent years, there has been increasing evidence that the physical properties of the extracellular matrix (ECM), such as surface topography, affect various cellular behaviors such as proliferation, adhesion, and migration. However, the biological mechanism of surface topography influencing cellular behavior is still unclear. In this study, we prepared polycaprolactone (PCL) fibrous materials with different surface microstructures by solvent casting, electrospinning, and self-induced crystallization. The corresponding topographical structure obtained is a two-dimensional (2D) flat surface, 2.5-dimensional (2.5D) fibers, and three-dimensional (3D) fibers with a multilevel microstructure. We then investigated the effects of the complex topographical structure on endothelial cell migration. Our study demonstrates that cells can sense the changes of micro-and nanomorphology on the surface of materials, adapt to the physical environment through biochemical reactions, and regulate actin polymerization and directional migration through Rac1 and Cdc42. The cells on the nanofibers are elongated spindles, and the positive feedback of cell adhesion and actin polymerization along the fiber direction makes the plasma membrane continue to protrude, promoting cell polarization and directional migration. This study might provide new insights into the biomaterial design, especially those used for artificial vascular grafts.

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Endothelial Cell Migration on Poly(ε-caprolactone) Nanofibers Coated with a Nanohybrid Shish-Kebab Structure Mimicking Collagen Fibrils

Cited by 36 publications

References 53 publications

Preparation of gelatin-based hydrogels with tunable mechanical properties and modulation on cell–matrix interactions

Preparation of gelatin-based hydrogels with tunable mechanical properties and modulation on cell–matrix interactions

Hierarchical Nanostructured Electrospun Membrane with Periosteum‐Mimic Microenvironment for Enhanced Bone Regeneration

Endothelial Cell Migration Regulated by Surface Topography of Poly(ε-caprolactone) Nanofibers

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