Endothelial Cell Damage Following Crystalloid Cardioplegic Solution Infusion

Harjula, A; Mattila, S; Järvinen, Antero; Myllärniemi, H; Salmenperä, Markku

doi:10.1001/archsurg.1984.01390200064015

“…We could recently show significant differences concerning the morphological integrity of venous and arterial endothelium monolayers after exposure to several crystalloid and cardioplegic Solutions, using light microscopy of the endothelial membrane [12], and the results confirmed the reports of others [5,13]. Moreover, a cytotoxic potential of crystalloid Solutions in endothelial cell eultures has been described by Carpentier [14].…”

Section: Discussionsupporting

confidence: 88%

“…The harmful effects of hydrostatic forces, of distension of bypass vessels during preparation, and of the low storage temperatures of some perfusion solutions have been stressed by many authors [2 -4]. However, little is known about the influence on the vessel endothelium of perfusion and preservation solutions used in bypass surgery, and this is offen contradictory [5,6]. Of main interest is the actual State of the endothelium after aortocoronary bypass surgery.…”

Section: Introductionmentioning

confidence: 99%

Influence of Several Solutions Used in Bypass Surgery on the Permeability of the Endothelium of Carotid Arteries in New Zealand Rabbits

Schaeffer

¹

,

Strohschneider

²

,

Pilch

³

et al. 1999

Thorac cardiovasc Surg

View full text Add to dashboard Cite

show abstract

“…Most obviously, CF in hearts stored for a long period in crystalloid buffers is known to be severely reduced upon reperfusion [1,13k15] and this has been attributed both to myocyte swelling [15] and to damage to the endothelial monolayer [16], particularly its glycocalyx [17]. Damage to the endothelium by crystalloid solutions may manifest itself as an impairment of endothelium-dependent relaxation in both large arteries and microvessels, possibly through a reduction in NO production [5].…”

Section: Discussionmentioning

confidence: 99%

Effect of sodium nitroprusside and L-arginine methyl ester on rat hearts stored at 4 °C for 24 h

Fagbemi

¹

,

Northover

²

1998

View full text Add to dashboard Cite

1. This study examined the effects of altering nitric oxide levels with sodium nitroprusside or L-arginine in rat hearts stored hypothermically.2. Hearts were microperfused at 4 degrees C for 24 h with a modified Krebs-Henseleit buffer (KHB) that contained either sodium nitroprusside, L-arginine, L-arginine methyl ester or dexamethasone.3. After hypothermic storage, hearts were rewarmed to 37 degrees C with KHB alone or KHB containing sodium nitroprusside or L-arginine. Cardiac function was then assessed in either Langendorff mode or working heart mode.4. Compared with values from fresh unstored hearts, hypothermic stored hearts showed a significant decrease in coronary flow and left ventricular developed pressure when the stored hearts were perfused in Langendorff mode. These hearts also produced less aortic flow and cardiac output when perfused in the working mode.5. Hearts hypothermically microperfused with buffer containing either L-arginine or sodium nitroprusside and then reperfused in the Langendorff mode with untreated KHB buffer had the highest left ventricular developed pressure and coronary flow values. Aortic flow and cardiac output were also higher in these hearts.6. In all groups of stored hearts, the concentrations of both ATP and creatine phosphate were significantly low, when compared with values from freshly isolated hearts. Addition of dexamethasone to the buffer either during storage or during reperfusion had no beneficial effect on high-energy phosphate loss or cardiac performance of stored hearts.7. This study showed that the addition of nitric oxide donors to storage buffer significantly improves cardiac function on normothermic reperfusion. The improved functional recovery is unrelated to the high-energy phosphate content of these hearts.

show abstract

“…Most obviously, CF in hearts stored for a long period in crystalloid buffers is known to be severely reduced upon reperfusion [1,13k15] and this has been attributed both to myocyte swelling [15] and to damage to the endothelial monolayer [16], particularly its glycocalyx [17]. Most obviously, CF in hearts stored for a long period in crystalloid buffers is known to be severely reduced upon reperfusion [1,13k15] and this has been attributed both to myocyte swelling [15] and to damage to the endothelial monolayer [16], particularly its glycocalyx [17].…”

Section: Discussionmentioning

confidence: 99%

Untitled

1998

Clinical Science

2

0

View full text Add to dashboard Cite

1. This study examined the effects of altering nitric oxide levels with sodium nitroprusside or L-arginine in rat hearts stored hypothermically.2. Hearts were microperfused at 4 degrees C for 24 h with a modified Krebs-Henseleit buffer (KHB) that contained either sodium nitroprusside, L-arginine, L-arginine methyl ester or dexamethasone.3. After hypothermic storage, hearts were rewarmed to 37 degrees C with KHB alone or KHB containing sodium nitroprusside or L-arginine. Cardiac function was then assessed in either Langendorff mode or working heart mode.4. Compared with values from fresh unstored hearts, hypothermic stored hearts showed a significant decrease in coronary flow and left ventricular developed pressure when the stored hearts were perfused in Langendorff mode. These hearts also produced less aortic flow and cardiac output when perfused in the working mode.5. Hearts hypothermically microperfused with buffer containing either L-arginine or sodium nitroprusside and then reperfused in the Langendorff mode with untreated KHB buffer had the highest left ventricular developed pressure and coronary flow values. Aortic flow and cardiac output were also higher in these hearts.6. In all groups of stored hearts, the concentrations of both ATP and creatine phosphate were significantly low, when compared with values from freshly isolated hearts. Addition of dexamethasone to the buffer either during storage or during reperfusion had no beneficial effect on high-energy phosphate loss or cardiac performance of stored hearts.7. This study showed that the addition of nitric oxide donors to storage buffer significantly improves cardiac function on normothermic reperfusion. The improved functional recovery is unrelated to the high-energy phosphate content of these hearts.

show abstract

Endothelial Cell Damage Following Crystalloid Cardioplegic Solution Infusion

Cited by 21 publications

References 4 publications

Influence of Several Solutions Used in Bypass Surgery on the Permeability of the Endothelium of Carotid Arteries in New Zealand Rabbits

Influence of Several Solutions Used in Bypass Surgery on the Permeability of the Endothelium of Carotid Arteries in New Zealand Rabbits

Effect of sodium nitroprusside and L-arginine methyl ester on rat hearts stored at 4 °C for 24 h

Untitled

Contact Info

Product

Resources

About