Endophytic microbial diversity in coffee cherries of<i>Coffea arabica</i>from southeastern Brazil

Oliveira, Marcelo Nagem Valério de; Santos, Thiago M. A.; Vale, Helson Mário Martins do; Delvaux, Júlio César; Cordero, Alexander Pérez; Ferreira, Alessandra Barbosa; Miguel, Paulo Sérgio Balbino; Tótola, Marcos Rogério; Costa, Maurício Dutra; Moraes, Célia Alencar de; Borges, Arnaldo Chaer

doi:10.1139/cjm-2012-0674

Cited by 50 publications

(30 citation statements)

References 50 publications

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“…Next, the material w as immersed tw ice in distilled w ater and phosphate buffer (0.05 mmol·L -1 ), pH 7, immersed in 70% ethanol (v/v) for one minute, kept in a container filled w ith sodium hypochlorite (5%) + 0.05% (v/v) Tw een-80 for five minutes, and then immersed for 30 s in 70% ethanol (v/v) before being immersed again in sodium hypochlorite + Tw een-80 for 15 min (Miguel et al, , 2016Oliveira et al, 2013). This process w as repeated once.…”

Section: Surface Sterilization Of the Eucalyptus Leavesmentioning

confidence: 99%

“…Microbial diversity can also be studied by analyzing the total DNA of the microbiota extracted directly from the plant for processing using electrophoretic techniques . Studies using these techniques have contributed to a better understanding of the microbial community structure and have led to new perspectives and advances in ecological studies (Hoshino and Matsumoto, 2007;Oliveira et al, 2013;Da Silva et al, 2014). Thus, the present study aimed at evaluating the composition and diversity of the endophytic fungal community in eucalyptus leaves at the onset of the rainy period and during the rainy and dry periods.…”

Section: Introductionmentioning

confidence: 99%

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Diversity and distribution of the endophytic fungal community in eucalyptus leaves

Miguel¹,

Delvaux²,

Oliveira³

et al. 2017

Afr. J. Microbiol. Res.

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Eucalyptus species are among the most widely grown and most economically valuable trees worldwide. In Brazil, eucalyptus is of major environmental and economic importance because it generates thousands of jobs and offers an alternative to using wood from native forests. In forest ecosystems, plant shoots are considered a common habitat for various microorganisms, and plants of the family Myrtaceae are an important source of fungal biodiversity. However, very little is known about the diversity and microbial distribution in eucalyptus leaves. This study aimed at showing the diversity and distribution of endophytic fungi in the leaves of eucalyptus plants aged 18 and 72 months. The leaves were collected at the onset of the rainy period, during the rainy period, and during the dry period. Diversity was measured using DNA extraction, 18S rRNA subunit amplifi cation, denaturing gradient gel electrophoresis (DGGE), and sequencing of eluted bands. The endophytic fungal community was affected by plant location. Differences observed in the distribution of the phylogenetic groups found in the upper, middle and lower thirds of the tree canopy indicate that the endophytic community distribution in eucalyptus is dependent on leaf position. The age of the plants affected the diversity of endophytic fungi in Eucalyptus "urograndis". Phylogenetic analysis showed that the phyla Basidiomycota and Ascomycota dominate in the environments studied. The description of endophytic fungal diversity in this important forest species is an important step for assessing this genetic resource in the search for metabolites and processes tha t can contribute to improving plant development.

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Section: Surface Sterilization Of the Eucalyptus Leavesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Diversity and distribution of the endophytic fungal community in eucalyptus leaves

Miguel¹,

Delvaux²,

Oliveira³

et al. 2017

Afr. J. Microbiol. Res.

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“…Archaea represent the so-called third domain of life, and have only recently been described as important component of the moderate environment and the human microbiome (Probst et al, 2013). A few very recent publications have mentioned internal plant tissue colonization by members of the Archaea (Ma et al, 2013; Oliveira et al, 2013), but their distribution, significance, function, and activity remains unclear. In addition, the endophytic microbiota of trees has undergone less investigation and nothing is known about the associated microorganisms within olive trees.…”

Section: Introductionmentioning

confidence: 99%

Plant genotype-specific archaeal and bacterial endophytes but similar Bacillus antagonists colonize Mediterranean olive trees

et al. 2015

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Endophytes have an intimate and often symbiotic interaction with their hosts. Less is known about the composition and function of endophytes in trees. In order to evaluate our hypothesis that plant genotype and origin have a strong impact on both, endophytes of leaves from 10 Olea europaea L. cultivars from the Mediterranean basin growing at a single agricultural site in Spain and from nine wild olive trees located in natural habitats in Greece, Cyprus, and on Madeira Island were studied. The composition of the bacterial endophytic communities as revealed by 16S rRNA gene amplicon sequencing and the subsequent PCoA analysis showed a strong correlation to the plant genotypes. The bacterial distribution patterns were congruent with the plant origins in “Eastern” and “Western” areas of the Mediterranean basin. Subsequently, the endophytic microbiome of wild olives was shown to be closely related to those of cultivated olives of the corresponding geographic origins. The olive leaf endosphere harbored mostly Proteobacteria, followed by Firmicutes, Actinobacteria, and Bacteroidetes. The detection of a high portion of archaeal taxa belonging to the phyla Thaumarchaeota, Crenarchaeota, and Euryarchaeota in the amplicon libraries was an unexpected discovery, which was confirmed by quantitative real-time PCR revealing an archaeal portion of up to 35.8%. Although the function of these Archaea for their host plant remains speculative, this finding suggests a significant relevance of archaeal endophytes for plant–microbe interactions. In addition, the antagonistic potential of culturable endophytes was determined; all isolates with antagonistic activity against the olive-pathogenic fungus Verticillium dahliae Kleb. belong to Bacillus amyloliquefaciens. In contrast to the specific global structural diversity, BOX-fingerprints of the antagonistic Bacillus isolates were highly similar and independent of the olive genotype from which they were isolated.

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“…These results extend current thinking, that multiple organisms within multiple taxonomic domains may be involved in the onset of disease or disease suppression in plants (Coats and Rumpho 2014). Most studies on microbial endophyte communities and dieback investigate one specific microbial taxon, typically fungi (Wilson and Pitkethley 1992;Diplock et al 2006;Kowalski and Holdenrieder 2009;Haque et al 2012;Ismail et al 2012;Sacdalan et al 2012), although there are exceptions (Mendes et al 2011;Knief et al 2012;Ma et al 2013;Oliveira et al 2013;Ruppel et al 2013). Our study found a very diverse microbial community (as assessed using OTUs) whose structure differed with plant health status.…”

Section: Discussionmentioning

confidence: 56%

Endophyte community composition is associated with dieback occurrence in an invasive tree

et al. 2015

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Background and aims Dieback is pervasive in many populations of invasive woody weeds globally. Previous studies on dieback have focused on specific potential causative biotic agents, but most cases remain unexplained. The potential role of endophytic microbial communities in dieback, including the relative importance of endophytes with pathogenic or protective capabilities, remains poorly studied. We tested whether changes in archaeal, bacterial and fungal endophyte community structure is associated with dieback occurrence in the invasive, leguminous tree, Parkinsonia aculeata L. (parkinsonia). Methods We sampled roots, stems and stem tips from healthy and dieback-affected parkinsonia and conducted terminal restriction fragment length polymorphism (T-RFLP) analysis on DNA extracted from these samples using domain-specific primers for archaea, bacteria and higher fungi.Results Microbial community composition strongly differed with parkinsonia disease status (archaea, bacteria and fungi) and plant part (archaea and fungi). Plant part and disease status effects were strongest in archaea. We also found evidence implicating both pathogenic and potentially protective endophytes in the onset of dieback. Conclusions This is the first study that has shown significant associations between changes in endophyte community composition and dieback presence. Our results highlight the complexity of those changes and provide support for the hypothesis that diverse pathogenic and protective endophytes may be implicated in dieback.

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Endophytic microbial diversity in coffee cherries ofCoffea arabicafrom southeastern Brazil

Cited by 50 publications

References 50 publications

Diversity and distribution of the endophytic fungal community in eucalyptus leaves

Diversity and distribution of the endophytic fungal community in eucalyptus leaves

Plant genotype-specific archaeal and bacterial endophytes but similar Bacillus antagonists colonize Mediterranean olive trees

Endophyte community composition is associated with dieback occurrence in an invasive tree

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