Endophytic Fusarium solani: A rich source of cytotoxic and antimicrobial napthaquinone and aza-anthraquinone derivatives

Khan, Noor; Afroz, Farhana; Begum, Mohajira; Rony, Satyajit Roy; Sharmin, Suriya; Moni, Fatema; Hasan, Choudhury M.; Shaha, Koushik; Sohrab, Md. Hossain

doi:10.1016/j.toxrep.2018.08.016

Cited by 61 publications

(55 citation statements)

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“…Stigmasterol may be a potential chemotherapeutic or a chemopreventive agent based on its ability to induce apoptosis or necrosis in transformed kidney cells or kidney (renal) cancer cells with relatively low toxicity to normal cells. Further studies with in vivo and clinical trials needs to be conducted to establish drugs for cancer therapy [16]. The brine shrimp lethality bioassay has been found as a safe, practical and economic method for determination of bioactivities of synthetic compound as well as plant products.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic, Antimicrobial and Antioxidant Properties of <i>Commelina diffusa</i> Burm. F.

Nasrin¹,

Afroz²,

Sharmin³

et al. 2019

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Commelina diffusa Burm. F. is a herbaceous tropical plant with different traditional medicinal uses. Present study is aimed to isolate the bioactive compounds from DCM-Methanol extract of the powdered whole plant of Commelina diffusa and to investigate the cytotoxic, antimicrobial and antioxidant activities of the crude extract and its twelve vacuum liquid chromatographic fraction (CD 1-12 ). Vacuum Liquid Chromatography (VLC) was employed to isolate bioactive metabolites. The physicochemical properties of the isolated compound were examined and the molecular structure was elucidated by NMR spectroscopy. Cytotoxic, antibacterial, antioxidant activities were evaluated following brine shrimp lethality bioassay, disc diffusion method and DPPH free radical scavenging assay respectively. The isolated compound was identified as steroid (stigmasterol) which had significant cytotoxic effect on vero cell line. The crude extract and its fractions (CD8-CD12) exhibited strong cytotoxicity in brine shrimp lethality bioassay having LC 50 values 3. 79, 9.19, 29.49, 16.60, 19.36, 44.58 μg/mL respectively. The crude extract showed mild to strong antibacterial activity. Fractions (CD5-CD12) showed mild to strong antibacterial activity against Escherichia coli in comparison with Kanamycin (standard). Strong antioxidant activities were found in crude extract (IC 50 value 30.52 μg/ml), CD11 (IC 50 value 39.27 μg/ml) and CD12 (IC 50 value 19.50 μg/ml). The study suggests Commelina diffusa plant extract to have strong antioxidant and cytotoxic activity which is indicative of presence of compounds with broad spectrum of curative applications. One compound namely stigmasterol has been isolated from the plant.

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Section: Discussionmentioning

confidence: 99%

Cytotoxic, Antimicrobial and Antioxidant Properties of <i>Commelina diffusa</i> Burm. F.

Nasrin¹,

Afroz²,

Sharmin³

et al. 2019

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“…Their chemical structures were elucidated by extensive NMR analysis (Supporting Materials). The chemical structures of four known compounds were identified as fusarester D (2) [26], karuquinone B (3) [27], javanicin (4) [28], solaniol (5) [29], and fusarubin (6) [30] by comparing with the published spectroscopic data (Figure 1).…”

Section: Identification Of Compounds 1-6mentioning

confidence: 99%

Neuroprotective γ-Pyrones from Fusarium Solani JS-0169: Cell-Based Identification of Active Compounds and an Informatics Approach to Predict the Mechanism of Action

et al. 2020

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Glutamate toxicity has been implicated in neuronal cell death in both acute CNS injury and in chronic diseases. In our search for neuroprotective agents obtained from natural sources that inhibit glutamate toxicity, an endophytic fungus, Fusarium solani JS-0169 isolated from the leaves of Morus alba, was found to show potent inhibitory activity. Chemical investigation of the cultures of the fungus JS-0169 afforded isolation of six compounds, including one new γ-pyrone (1), a known γ-pyrone, fusarester D (2), and four known naphthoquinones: karuquinone B (3), javanicin (4), solaniol (5), and fusarubin (6). To identify the protective effects of the isolated compounds (1–6), we assessed their inhibitory effect against glutamate-induced cytotoxicity in HT22 cells. Among the isolates, compound 6 showed significant neuroprotective activity on glutamate-mediated HT22 cell death. In addition, the informatics approach using in silico systems pharmacology identified that compound 6 may exert its neuroprotective effect by controlling the amount of ubiquinone. The results suggest that the metabolites produced by the endophyte Fusarium solani JS-0169 might be related to the neuroprotective activity of its host plant, M. alba.

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“…For the identification of endophytic fungal strains, slides were prepared from 02 days cultures by staining with lactophenol cotton blue. The prepared slides were examined for morphological characteristics by checking under a bright-field and phase-contrast microscope concerning different standard taxonomic keys 9,10 .…”

Section: Morphologicalmentioning

confidence: 99%

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2020

IJPSR

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Endophyte is a rich source of bioactive metabolites for medicinal exploitation as an increasing number of novel compounds with bioactive potential are now being isolated from this source. This study was conducted to characterize and explore endophytic fungi from marine weeds available in Bangladesh. The whole weed was used for isolation of endophytic fungi in the water agar media and the isolated fungi were identified through macroscopic and microscopic observation. Small scale cultivation was performed on liquid Wickersham media. Cytotoxicity, antibacterial, antioxidant activities were evaluated following brine shrimp lethality bioassay, disc diffusion method and DPPH free radical scavenging assay, respectively. A total of five endophytic fungi were isolated, purified and characterized from four seaweeds; Hypnea musciformis (HM), Sargassum crassifolium (SC), Dictyota dichotoma (DD) and Caulerpa peltata (CP). They were coded as HMWE-1, HMWE-2, SCWE-1, DDWE-1 and CPWE-1. The fungal strains HMWE-1 and SCWE-1 were identified morphologically as Fusarium sp., HMWE-2 and DDWE-1 as Penicillium sp. and CPWE-1 as Aspergillus sp. DDWE-1 fungal strain showed significant antioxidant activity in comparison to the standard, ascorbic acid. The fungal extracts CPWE-1 (LC 50 value of 2.85 µg/mL) and DDWE-1 (LC 50 value of 6.38 µg/mL) showed significant cytotoxic activity. The extracts exhibited weak to good activity against a wide range of human pathogenic microorganisms during anti-microbial activity screening. Preliminary chemical screening by thin-layer chromatography (TLC) indicated the presence of a number of secondary metabolites. Thus, this study explored the potential entophytes from marine weeds of the Bay of Bengal as a producer of leads for drugs.

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Endophytic Fusarium solani: A rich source of cytotoxic and antimicrobial napthaquinone and aza-anthraquinone derivatives

Abstract: Graphical abstract

Cited by 61 publications

References 25 publications

Cytotoxic, Antimicrobial and Antioxidant Properties of <i>Commelina diffusa</i> Burm. F.

Cytotoxic, Antimicrobial and Antioxidant Properties of <i>Commelina diffusa</i> Burm. F.

Neuroprotective γ-Pyrones from Fusarium Solani JS-0169: Cell-Based Identification of Active Compounds and an Informatics Approach to Predict the Mechanism of Action

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Endophytic Fusarium solani: A rich source of cytotoxic and antimicrobial napthaquinone and aza-anthraquinone derivatives

Abstract: Graphical abstract

Cited by 61 publications

References 25 publications

Cytotoxic, Antimicrobial and Antioxidant Properties of &lt;i&gt;Commelina diffusa&lt;/i&gt; Burm. F.

Cytotoxic, Antimicrobial and Antioxidant Properties of &lt;i&gt;Commelina diffusa&lt;/i&gt; Burm. F.

Neuroprotective γ-Pyrones from Fusarium Solani JS-0169: Cell-Based Identification of Active Compounds and an Informatics Approach to Predict the Mechanism of Action

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Cytotoxic, Antimicrobial and Antioxidant Properties of <i>Commelina diffusa</i> Burm. F.

Cytotoxic, Antimicrobial and Antioxidant Properties of <i>Commelina diffusa</i> Burm. F.