Endophytic fungi from Chilean native gymnosperms: antimicrobial activity against human and phytopathogenic fungi

Abstract

Thirty-eight endophytic fungi were isolated from eight Chilean gymnosperms. Isolates were characterized and grouped according to culture characteristics, colony growth, and conidia morphology. Thirteen isolates were identified: Acremonium bacillisporum, A. bactrocephalum, A. strictum, Alternaria alternata, Aureobasidium pullulans, Chaetomium funicola, Cladosporium tenuissimum, Curvularia protuberata, C. tritici, Microsphaeropsis olivacea, Penicillium chrysogenum, P. janczewskii, and Triblidiopycnis pinastri. Malbranchea and Stegonosporium were identified at the genus level. Fourteen isolates, considered to be sterile mycelia, did not fructify in the culture medium. Crude extracts of liquid cultures from endophytes were examined for antibacterial and antifungal activity against bacteria and phytopathogenic fungi using agar diffusion. Antifungal activity against pathogenic fungi was determined by microdilution assays. Extracts of Acremonium bactrocephalum, Microsphaeropsis olivacea, and isolate E-3 inhibited growth of selected pathogenic organisms, indicating they merit further study. This is the first comparative report on the antimicrobial activity of endophytic fungi from Chilean gymnosperms.

Isolation and evaluation of endophytic fungi with antimicrobial ability from <i>Phyllostachys edulis </i>

“…The mycelium was extracted with ethyl acetate (EtOAc) in order to obtain bioactive extracts (Hormazabal and Piontelli, 2009).…”

Endophytic microorganisms from Bauhinia monandra leaves: Isolation, antimicrobial activities and interaction with galactose-specific lectin BmoLL

“…Endophytic microorganisms have been isolated from different plant species and they are reported as agents in biological control of plant diseases and plagues (Hormazabal and Piontelli, 2009;Vendan et al, 2010;Jin et al, 2014). Endophytes have potential applications in agriculture, industry and medicine (Brader et al, 2014).…”

“…They live within the intercellular spaces of plants, in tissues such as roots, stems and leaves (Hormazabal and Piontelli, 2009). They invade the vegetal in different stages of development, but do not cause symptoms of diseases.…”

Isolation and Characterization of Saponin-Producing Fungal Endophytes from Aralia elata in Northeast China

“…The antimicrobial activity of typical strains after 14-day cultivation against 8 microorganisms (listed in Table 3) was assessed by the agar diffusion method [33]. Three 6 mm wells were made in each disk.…”