Isolation and evaluation of endophytic fungi with antimicrobial ability from &lt;i&gt;Phyllostachys edulis &lt;/i&gt;

Shen, Xiaoye; Zheng, Dongqi; Gao, Jian; Hou, Cheng‐Lin

doi:10.3329/bjp.v7i4.12068

Cited by 24 publications

(21 citation statements)

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“…were identified as fungal endophytes. [8] In contrast to hypocrellin biosynthesis strains generally originating from the fruiting body of S. bambusicola Henn., we screened the hypocrellin-producing strains based on these endophytic fungi, and some of them exhibited significantly high content of hypocrellins, compared to the unmodified strains from the traditional resource of the fruiting body. As far as we know, it is the first time that the industrial strains of Shiraia sp.…”

Section: Resultsmentioning

confidence: 96%

“…Hypocrellins inhibited the growth of phytopathogens (Arthrinium sacchari, A. Phaeospermum, B. fuckeliana and T. cucumeris) considerably and displayed low toxicity, low aggregation and rapid metabolism in vivo, as a promising green pesticide. [8] Similarly, owing to antiinflammatory activity, analgesic activity and low toxicity, hypocrellins also take a vital part in the skin care product "Jianfushuang", which has been granted as the earliest patent in the cosmetic industries in China (Publication Number: CN85103693). The recent study has been carried out that the extent of encapsulation from CONTACT Cheng-Lin Hou houchenglincn@yahoo.com; chenglin-hou@cnu.edu.cn hypocrellin liposome could be achieved by the best way macroporous resin column chromatography.…”

Section: Introductionmentioning

confidence: 99%

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Improvement of hypocrellin production by a new fungal source and optimization of cultivation conditions

Shen

Song

et al. 2016

Biotechnology & Biotechnological Equipment

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Hypocrellins is regarded as the new generation of potential photosensitizer in the photodynamic therapy. However, the current production of HA in large quantity is limited, whether extracted from the fruit bodies of Hypcrella bambusae and Shiraia bambusicola, or its fermented mycelia. To break through the bottleneck, we screened over 350 fungal strains from the uncommon moso bamboo (Phyllostachys edulis) seeds, and acquired 14 isolates of Shiraia sp. Hypocrellins were generally extracted from the stroma of S. bambusicola, and however the hypocrellin-producing strains in our study were identified as endophytic fungi, where several of them exhibited significantly high content of hypocrellins. One of them, a newly isolated Shiraia sp. strain ZZZ816 was selected for further research. The hypocrellin production conditions of the strain ZZZ816 under submerged fermentation were optimized by one-factor-at-a-time and orthogonal experiment design. The optimum parameters for the quid fermentation were determined as follows: incubation time (144 h), initial pH (6.0), volume of medium (100/250 mL), rotary speed (130 rpm), mycelial age (60 h) and inoculation level (10%). The optimum culture medium constituents were determined as follows: yeast extract 20g/L, malt sugar 40g/L, FeSO 4 ¢H 2 O 0.5g/L, urea 4.0g/L, MgS0 4 ¢7H 2 0 0.5g/L and initial pH 5.0. Using submerged fermentation of the optimum production conditions, the maximum content of hypocrellin A from the strain ZZZ816 was 921.6 mg/L, more than the other industrial strains published in the previous reports. The results demonstrated that Shiraia sp. strain ZZZ816 could enhance the hypocrellin production tremendously with optimizing the fermentation progress.

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Section: Resultsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Improvement of hypocrellin production by a new fungal source and optimization of cultivation conditions

Shen

Song

et al. 2016

Biotechnology & Biotechnological Equipment

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“…Similar results were obtained by Zhang et al (2013a) in a study of fungi associated with Taraxacum mongolicum, and the authors also found positive results against S. aureus (40 mm). Shen et al (2012) has isolated endophytes from branches of Phyllostachys edulis and tested P. herbarum extracts against bacteria; it was not able to inhibit S. aureus and B. subtilis. The same fact occurred according to Pharamat et al (2013) …”

Section: Discussionmentioning

confidence: 99%

Antimicrobial activity of Phoma sp. URM 7221: An endophyte from Schinus terebinthifolius Raddi (Anacardiaceae)

Silva¹,

Silvino²,

Bezerra³

et al. 2017

Afr. J. Microbiol. Res.

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The discovery of new metabolites potentially bioactive against pathogenic microorganisms, mainly multidrug resistant, has aroused interest in endophytic fungi. The plant-associated microorganisms have been an important source for development of new compounds of biotechnological interest. This study aimed to investigate the antibacterial capacity of the endophytic fungus, Phoma sp. URM 7221 isolated from the medicinal plant Schinus terebinthifolius against human-pathogenic bacteria. An endophyte was isolated from S. terebinthifolius leaves. Phoma herbarum URM7221 was characterized morphologically and on the basis of ITS rDNA sequence. Primary antimicrobial activity was evaluated using the agar diffusion method and fermentation in liquid medium. Six different solvents were used to extract the active metabolites from fungal biomass and metabolic liquid. An antimicrobial activity test from the extract was carried out using a disk diffusion method with the endophytic extract containing the best antibacterial activity. Two tests were performed: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Partially purified secondary metabolite extracts were analysed by thin layer chromatography (TLC). Liquid metabolically bioactive compounds extracted with petroleum ether revealed a MIC of 25 and 500 μg.mL −1 against S. aureus and MRSA, respectively. Ether and methanol extracts were assessed by chemical analyses and contained phenolic compounds, triterpenes, steroids, reducing sugars, mono-and sesquiterpenes. The thin layer chromatography assay showed the activity of different antimicrobial compounds produced by Phoma sp. URM7221. This endophyte (URM7221) could be efficiently used for production of bioactive metabolites against pathogenic microorganisms, with significant biotechnological potential.

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“…Endophytes can be found in almost all plants and play a vital role in the growth of host plants against stressed condition (Li et al, 2013;Yu et al, 2017). Endophytes have the major impact on drug discovery (Awad et al, 2014) since it proved to be a valuable source of novel and structurally diverse secondary metabolites which may possess various biological potentials such as antimicrobial (Shen et al, 2012;Zhang et al, 2012;Zhang et al, 2015), anti-cancer (Shylaja and Sathiavelu 2017), immunosuppressant and others (Zhang et al, 2014; on the isolation and identification of bioactive metabolite of the endophytic fungus C. cupreum from M. luteola and to evaluate the anti-oxidant, antibacterial and antimycobacterial potentials.…”

Section: Introductionmentioning

confidence: 99%

Antimycobacterial potential of resorcinol type lipid isolated from Chaetomium cupreum, an endophytic fungus from Mussaenda luteola

Shylaja

Sasikumar

Arunachalam

2018

Bangladesh J Pharmacol

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An endophytic fungus Chaetomium cupreum was isolated from the ornamental plant Mussaenda luteola. A known metabolite named resorcinol type of lipid (compound 1) was isolated using column chromatography and structurally elucidated by spectroscopic studies includes UV-Vis, FT-IR, NMR, MS analysis and comparing with the existing data. The compound 1 was evaluated for antimycobacterial potential by microtitre plate alamar blue assay against Mycobacterium tuberculosis H37Rv (ATCC27294). It also estimated for DPPH free radical scavenging and antibacterial (agar well diffusion method) potentials. Compound 1 revealed to have significant inhibition of Mycobacterium with MIC of 6.3 µg/mL which is similar to the standard streptomycin drug. It also exhibited good DPPH free radical scavenging potential of 84.5 ± 0.4% and maximum inhibition of both E. coli (ATCC 25922) as well as S. aureus (ATCC 25923). Thus, endophytic fungus C. cupreum from Mussaenda luteola could be a potential source of novel lead antimicrobial agents for drug discovery.Video Clip of Methodology:9 min 43 sec: <a href="https://www.youtube.com/v/jVjoCzsGMg8">Full Screen</a> <a href="https://www.youtube.com/watch?v=jVjoCzsGMg8">Alternate</a>

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Isolation and evaluation of endophytic fungi with antimicrobial ability from <i>Phyllostachys edulis </i>

Cited by 24 publications

References 53 publications

Improvement of hypocrellin production by a new fungal source and optimization of cultivation conditions

Improvement of hypocrellin production by a new fungal source and optimization of cultivation conditions

Antimicrobial activity of Phoma sp. URM 7221: An endophyte from Schinus terebinthifolius Raddi (Anacardiaceae)

Antimycobacterial potential of resorcinol type lipid isolated from Chaetomium cupreum, an endophytic fungus from Mussaenda luteola

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