Endophytic fungi found in association with Bacopa monnieri as potential producers of industrial enzymes and antimicrobial bioactive compounds

Katoch, Meenu; Salgotra, Aseem; Singh, Gurpreet

doi:10.1590/s1516-8913201402502

Cited by 32 publications

(23 citation statements)

References 24 publications

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“…Among the endophytes from medicinal plants, many studies have revealed Fusarium sp. as the most prevalent species and a potent source of biologically active compounds (37,38). Many studies have been carried out on metabolites production from several Fusarium species especially F. oxysporum and F. solani (39)(40)(41)(42).…”

Section: Discussionmentioning

confidence: 99%

Toxic, but beneficial compounds from endophytic fungi of Carica papaya

Eze

Abonyi

Abba

et al. 2019

The EuroBiotech Journal

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Fungi remain a promising source of novel biologically active compounds with potentials in drug discovery and development. This study was aimed at investigating the secondary metabolites from endophytic Fusarium equiseti and Epicoccum sorghinum associated with leaves of Carica papaya collected from Agulu, Anambra State, Nigeria. Isolation of the endophytic fungi, taxonomic identification, fermentation, extraction and isolation of fungal secondary metabolites were carried out using standard procedures. Chromatographic separation and spectroscopic analyses of the fungal secondary metabolites yielded three toxigenic compounds - equisetin and its epimer 5’- epiequisetin from F. equiseti and tenuazonic acid from E. sorghinum These compounds are known to possess several beneficial biological properties that can be explored for pharmaceutical, agricultural or industrial purposes.

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Section: Discussionmentioning

confidence: 99%

Toxic, but beneficial compounds from endophytic fungi of Carica papaya

Eze

Abonyi

Abba

et al. 2019

The EuroBiotech Journal

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Section: Introductionmentioning

confidence: 99%

“…Most of the studies regarding the production of plant degrading enzymes by endophytic fungi involve cultivation on solid media and qualitative evaluation of substrates hydrolysis (Sunitha et al, 2013) (Katoch et al, 2014). Quantitative analysis by submerged fermentation is also cited (Katoch et al, 2014). However, studies using solid-state fermentation (SSF) for this purpose are scarce and involve only some of the most commonly studied endophytic genera such as Acremonium, Alternaria, Aspergillus, Chaetomium and Penicillium (Almeida et al, 2011;Onofre et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse

Marques

Pereira

Gomes

et al. 2018

Industrial Crops and Products

105

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A B S T R A C TEndophytic fungi are widely studied as producers of secondary metabolites of biotechnological interest. In recent years, the interest in these fungi as new sources of enzymes, especially hydrolytic, has increased. In the present study, 14 strains of endophytic fungi not yet explored as enzymes sources were randomly chosen and prospected for cellulases and xylanases production by solid-state fermentation. Initially, fungi were cultivated in a mixture (1:1 w/w) of sugarcane bagasse and wheat bran for 7 days, at 28°C. In this initial screening, 4 fungi excelled in endoglucanase activity (U/g): Cladosporium cladosporioides PAJ 03 (88.51 ± 1.0), Phomopsis stipata SC 04 (83.44 ± 7.7), Trichoderma viridae PAJ 01 (64.56 ± 4.0) and Botryosphaeria sp. AM 01 (42.79 ± 1.6). On the other hand, the following 4 fungi stood out in relation to β-glucosidase activity (U/g): Saccharicola sp. EJC 04 (51.56 ± 2.7), Paecilomyces sp. SF 021 (33.19 ± 9.2), Ustilaginoidea sp. CV 04 (29.75 ± 0.8) and Ustilaginoidea sp. XYA 04 (21.72 ± 3.05). Among these fungi, P. stipata SC 04 and Botryosphaeria sp. AM 01 were the best producers of xylanase and β-xilosidase (694,33 and 4,87 U/g, respectively). These 8 fungi were then cultured in new mixtures (1:1 w/w) of lignocellulosic substrates. Botryosphaeria sp. AM01and Saccharicola sp. EJC04 stood out regarding endoglucanase and β-glucosidase activities (184.74 ± 6.0 and 92.28 ± 9.57 U/g, respectively) when cultivated on cotton seed meal and wheat bran and were selected to continue the study. The influence of time cultivation, inoculum amount and substrate initial moisture content was evaluated and the best condition for cellulases production was 192 h, six mycelial plugs and 65%, respectively, for both fungi. Cellulases and xylanases produced under these conditions were characterized and optimum pH and temperature values were between 4.5-6 and 60-75°C, respectively. The enzymes were stable over a wide pH range and under 30-70°C. β-glucosidase from both isolates retained about 75-80% of their activity in the presence of glucose at 6 mM. The presence of ethanol stimulated β-glucosidase activity from Botryosphaeria sp. AM01 (about 60% higher in the presence of ethanol at 15%). On the other hand, the activity of β-glucosidase produced by Saccharicola sp. EJC 04 was reduced at ethanol concentrations above 15%. A blend of the enzymatic extracts was used to saccharify pretreated sugarcane bagasse and a face-centered central composite design was used to find the best conditions. Under the predicted optimum condition (50°C, 5% of sugarcane bagasse, 150 U g −1 of endoglucanase and 20 h), glucose and xylose concentrations obtained were 3.56 and 1.66 mg mL −1 , respectively. These results show that the 14 endophytic fungi studied have potential to be explored as producers of plant material degrading enzymes. Botryosphaeria sp. AM01 and Saccharicola sp. EJC 04 are promising in relation

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“…Fusarium was isolated only in PDA medium, Botrytis and Didymostilbe in PDA+extract, and Guignardia, Asterosporium, and Aureobasidium in SDA, showing the need to use different nutritional sources to increase the recovery rate and richness of endophytic fungi. Studies on endophytic fungal isolation usually use only PDA medium (Hilarino et al, 2011;Katoch et al, 2014;Bezerra et al, 2015).…”

Section: Isolation and Identification Of Endophytic Fungimentioning

confidence: 99%

“…Colletotrichum isolated as endophytic fungus also showed antibacterial activity against gram-positive, gramnegative, and Candida albicans bacteria (Katoch et al, 2014;Ferreira et al, 2015).…”

Section: Antibacterial Activitymentioning

confidence: 99%

Antibacterial activity of endophytic fungi from the medicinal plant Uncaria tomentosa (Willd.) DC

Rodrigues¹,

Araujo²,

Cunha³

et al. 2018

J. Med. Plants Res.

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This study was designed to determine the diversity and antibacterial activity of endophytic fungi isolated from Uncaria tomentosa. Leaf and stem were disinfected superficially and inoculated in PDA and SDA medium, with and without plant extract and incubated at 18 and 28°C for isolation of endophytic fungi. Endophytic fungi were inoculated in BD medium and the metabolites extracted with ethyl acetate. Endophytic fungi extracts were tested for antibacterial activity by the disk diffusion test. One hundred and seventy endophytic fungi were isolated and identified as Aspergillus, Asterosporium, Aureobasidium, Botrytis, Colletotrichum, Curvularia, Didymostilbe, Fusarium, Guignardia, Nigrospora, Penicillium, Pestalotiopsis, Phomopsis, and sterile mycelium. Staphylococcus aureus was the most resistant bacterium, with only two fungal extracts inhibiting its growth, while the most sensitive was Escherichia coli, with 23 extracts inhibiting its growth. Five extracts inhibited Enterococcus faecalis and four Klebsiella pneumoniae. No fungal extract was able to inhibit the four tested bacteria. Extracts from endophytic fungi isolated from U. tomentosa showed in vitro antibacterial activity against grampositive and gram-negative bacteria.

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Endophytic fungi found in association with Bacopa monnieri as potential producers of industrial enzymes and antimicrobial bioactive compounds

Abstract: ABSTRACT

Cited by 32 publications

References 24 publications

Toxic, but beneficial compounds from endophytic fungi of Carica papaya

Toxic, but beneficial compounds from endophytic fungi of Carica papaya

Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse

Antibacterial activity of endophytic fungi from the medicinal plant Uncaria tomentosa (Willd.) DC

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