Endophytic bacterium Sphingomonas SaMR12 promotes cadmium accumulation by increasing glutathione biosynthesis in Sedum alfredii Hance

Pan, Fengshan; Meng, Qin; Wang, Qiong; Luo, Sha; Bao, Chen; Khan, Kiran Yasmin; Yang, Xiaoe

doi:10.1016/j.chemosphere.2016.03.120

Cited by 91 publications

(41 citation statements)

References 47 publications

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“…Therefore, both the present and previous studies indicate that endophytic fungi can strengthen GSH metabolism under stress, which is considered an important mechanism for improving plant tolerance to environmental stress by endophytic fungi in roots (Waller et al 2005;Baltruschat et al 2008;Pan et al 2016). However, the physiological mechanisms by which DSEs enhance the Cd tolerance of their host plant still are poorly known and require additional study.…”

Section: Discussionmentioning

confidence: 53%

“…Therefore, an improvement in GSH metabolism in plants induced by endophytic fungi is considered to play an important role. For instance, the inoculation of endophytic Sphingomonas SaMR12 increased the GSH concentration and subsequently improved Cd tolerance and accumulation in the roots of Sedum alfredii (Pan et al 2016). The endophytic F. mosseae reduced the Cd content in the leaves and roots by increasing the GSH content in tobacco plants (Degola et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…These results indicate that the thiol compound content increase is related to the activation of the GR and GSH-Px activity in the maize leaves, induced by DSE colonization. Similarly, the GSH content increase was considered relevant to the up-regulated expression of relevant genes such as ATPS, GS and GSH1, which were induced by the endophytic Sphingomonas SaMR12 (Pan et al 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Under Cd stress, endophytes can also enhance plant fitness and alleviate Cd toxicity by regulating GSH metabolism and thiol compound contents in the host plant. For instance, the endophyte Sphingomonas SaMR12 up-regulates the expression of GSH synthesis-relevant genes (ATPS, GS and GSH1), increases GSH concentrations and subsequently enhances both Cd tolerance and accumulation (Pan et al 2016). Similarly, the AM fungus Funneliformis mosseae decreases leaf Cd contents with increasing GSH levels in tobacco (Degola et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Effects of a root-colonized dark septate endophyte on the glutathione metabolism in maize plants under cadmium stress

Zhan

Ming

et al. 2017

Journal of Plant Interactions

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A high Cd-tolerant dark septate endophyte (DSE), Exophiala pisciphila, was inoculated into maize (Zea mays L.) roots under Cd stress. The Cd content, enzymes activity and thiol compound content relevant to glutathione (GSH) metabolism in maize leaves were analyzed. The Cd content in maize shoots increased with increasing Cd stress, but the DSE significantly reduced the Cd content at the 40 mg/ kg Cd treatment. Cd stress increased the enzyme activity of glutathione reductase (GR), glutathione S-transferase (GST) and glutathione peroxidase (GSH-Px) as well as the thiol compound contents of sulfur, thiols (-SH) and oxidized glutathione (GSSG). The content of reduced GSH and the GSH/GSSG ratio reached a peak at the 5 mg/kg Cd treatment but then decreased with increasing Cd stress. Furthermore, the DSE significantly enhanced the GR and GSH-Px activity and increased the contents of-SH and GSH under low Cd stress (5 and 10 mg/kg), but decreased the γglutamylcysteine synthetase and GST activity under high Cd stress (20 and 40 mg/kg). Highly positive correlations between the Cd content with enzymes activity and enzymes activity with thiol compound content were observed. Results indicated that DSE played a role in activating GSH metabolism in maize leaves under Cd stress.

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of a root-colonized dark septate endophyte on the glutathione metabolism in maize plants under cadmium stress

Zhan

Ming

et al. 2017

Journal of Plant Interactions

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“…Recently, it has been shown that glutathione plays a positive role in alleviating Cd-mediated changes to different leaf sections in cotton (Daud et al, 2016). In Sedum alfredii Hance, the endophytic bacterium Sphingomonas SaMR12 promotes cadmium accumulation by increasing glutathione biosynthesis (Pan et al, 2016). In Arabidopsis , several genes, such as MAN3 and ZAT6 , regulate Cd tolerance via a glutathione-dependent pathway (Chen J. et al, 2015; Chen et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Cadmium-Treated Roots in Maize (Zea mays L.)

et al. 2016

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Cadmium (Cd) is a heavy metal and is highly toxic to all plant species. However, the underlying molecular mechanism controlling the effects of auxin on the Cd stress response in maize is largely unknown. In this study, the transcriptome produced by maize ‘Zheng 58’ root responses to Cd stress was sequenced using Illumina sequencing technology. In our study, six RNA-seq libraries yielded a total of 244 million clean short reads and 30.37 Gb of sequence data. A total of 6342 differentially expressed genes (DEGs) were grouped into 908 Gene Ontology (GO) categories and 198 Kyoto Encyclopedia of Genes and Genomes terms. GO term enrichment analysis indicated that various auxin signaling pathway-related GO terms were significantly enriched in DEGs. Comparison of the transcript abundances for auxin biosynthesis, transport, and downstream response genes revealed a universal expression response under Cd treatment. Furthermore, our data showed that free indole-3-acetic acid (IAA) levels were significantly reduced; but IAA oxidase activity was up-regulated after Cd treatment in maize roots. The analysis of Cd activity in maize roots under different Cd and auxin conditions confirmed that auxin affected Cd accumulation in maize seedlings. These results will improve our understanding of the complex molecular mechanisms underlying the response to Cd stress in maize roots.

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Neopolyploidy has variable effects on the diversity and composition of the wild strawberry microbiome

Anneberg,

Cullen,

O'Neill

et al. 2024

American J of Botany

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PremiseWhole‐genome duplication (neopolyploidy) can instantly differentiate the phenotype of neopolyploids from their diploid progenitors. These phenotypic shifts in organs such as roots and leaves could also differentiate the way neopolyploids interact with microbial species. While some studies have addressed how specific microbial interactions are affected by neopolyploidy, we lack an understanding of how genome duplication affects the diversity and composition of microbial communities.MethodsWe performed a common garden experiment with multiple clones of artificially synthesized autotetraploids and their ancestral diploids, derived from 13 genotypes of wild strawberry, Fragaria vesca. We sequenced epiphytic bacteria and fungi from roots and leaves and characterized microbial communities and leaf functional traits.ResultsAutotetraploidy had no effect on bacterial alpha diversity of either organ, but it did have a genotype‐dependent effect on the diversity of fungi on leaves. In contrast, autotetraploidy restructured the community composition of leaf bacteria and had a genotype‐dependent effect on fungal community composition in both organs. The most differentially abundant bacterial taxon on leaves belonged to the Sphingomonas, while a member of the Trichoderma was the most differentially abundant fungal taxon on roots. Ploidy‐induced change in leaf size was strongly correlated with a change in bacterial but not fungal leaf communities.ConclusionsGenome duplication can immediately alter aspects of the plant microbiome, but this effect varies by host genotype and bacterial and fungal community. Expanding these studies to wild settings where plants are exposed continuously to microbes are needed to confirm the patterns observed here.

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Endophytic bacterium Sphingomonas SaMR12 promotes cadmium accumulation by increasing glutathione biosynthesis in Sedum alfredii Hance

Cited by 91 publications

References 47 publications

Effects of a root-colonized dark septate endophyte on the glutathione metabolism in maize plants under cadmium stress

Effects of a root-colonized dark septate endophyte on the glutathione metabolism in maize plants under cadmium stress

Transcriptome Analysis of Cadmium-Treated Roots in Maize (Zea mays L.)

Neopolyploidy has variable effects on the diversity and composition of the wild strawberry microbiome

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