Effects of a root-colonized dark septate endophyte on the glutathione metabolism in maize plants under cadmium stress

Zhan, Fangdong; Li, Bo; Ming, Jiang; Li, Qin; Wang, Jixiu; He, Yongmei; Li, Yuan

doi:10.1080/17429145.2017.1385868

Cited by 30 publications

(23 citation statements)

References 60 publications

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“…Research has shown that endophytic fungi inhabit most plant roots in habitats polluted by Cd (Li et al, 2012;Yu et al, 2019). The significant effects of the endophytic fungi on plant growth and Cd tolerance have also been demonstrated (He et al, 2017;Zhan et al, 2017). In the present study, the hyphae and the microsclerotial structures of the DSEs were observed in all inoculated M. sativa and A. mongolicus roots after harvesting.…”

Section: Effects Of Dse Inoculation On Non-host Plantssupporting

confidence: 72%

Dark Septate Endophytes Improve the Growth and the Tolerance of Medicago sativa and Ammopiptanthus mongolicus Under Cadmium Stress

Hou

Zhao

et al. 2020

Front. Microbiol.

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Although the ecological function of dark septate endophytes (DSEs) is well studied, little is known about the responses of the host plant to DSEs obtained from other plants, especially under conditions of heavy metal stress. This study aimed to investigate how DSEs from a heavy-metal habitat affect non-host plants in cadmium (Cd) stress soils, which then provides a basis for the application of DSEs in the cultivation of different plant and soil remediation strategies for polluted ecosystems. We isolated and identified two species of DSE (Acrocalymma vagum and Scytalidium lignicola) inhabiting the roots of Ilex chinensis (host plant) which are grown in metal-polluted habitats. Then, the Cd stress tolerance of the DSEs was tested using a pure culture of which the Cd concentration has been adjusted. Subsequently, we examined the performance of non-host plants (Medicago sativa and Ammopiptanthus mongolicus) which were inoculated with DSEs under Cd stress in a growth chamber. The results indicated that the two DSEs could grow under Cd stress in vitro, even when not exhibiting high levels of tolerance to Cd. The superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), soluble protein, and melanin of the DSE fungi reached maximal levels at concentrations of 30-60 mg Cd/L, indicating the important preventive strategies adopted by the DSE fungi in environments contaminated by Cd. Despite a decreased biomass of DSE hyphae with enhanced Cd concentrations, the accumulation of Cd in the DSE hyphae tended to show an increasing trend. Both DSEs were effective colonizers of the non-host plants. A. vagum and S. lignicola inoculation significantly promoted the biomass and the root architecture of the two non-host plants under Cd stress. A. vagum inoculation increased the total nitrogen (TN) of A. mongolicus, whereas inoculation with S. lignicola significantly increased the organic carbon (OC) of M. sativa. In particular, the DSE inoculation significantly improved the accumulation of Cd in plant tissues under Cd stress, demonstrating a potential application in the bio-remediation of heavy-metalpollution areas. Our findings suggest that the DSE inoculation improved the root growth and nutrient absorption of non-host plants, altered the soil Cd concentration, and facilitated plant growth and survival under Cd stress. These results contribute to a better understanding of DSE-plant interactions in habitats contaminated by heavy metals.

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Section: Effects Of Dse Inoculation On Non-host Plantssupporting

confidence: 72%

Dark Septate Endophytes Improve the Growth and the Tolerance of Medicago sativa and Ammopiptanthus mongolicus Under Cadmium Stress

Hou

Zhao

et al. 2020

Front. Microbiol.

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“…In general, the Cd concentration was relatively less in the aboveground and belowground tissues of E+ plants in most of the treatments ( Table 1 ). This finding was similar to the results of the previous studies that observed lower Cd in inoculated plants than non-inoculated plants ( Wang et al, 2016 ; He et al, 2017 ; Zhan et al, 2017 ; Zhu et al, 2018 ; Shahid et al, 2019 ). However, in this study, the difference was non-significant ( p > 0.05) in most of the tested conditions ( Table 1 ).…”

Section: Discussionsupporting

confidence: 92%

“…In this study, GSH content of E+ plants was more than that of E− plants (Figure 3), which indicates the counteractive mechanism developed by inoculated endophyte to check oxidative stress due to Cd. The results were consistent with previous studies, which observed endophytic inoculation may improve the host plant growth and tolerance to Cd stress through GSH regulation in different host plants (Khan et al, 2015;Wang et al, 2016;He et al, 2017;Zhan et al, 2017;Zhang et al, 2019).…”

Section: Physiological and Biochemical Changessupporting

confidence: 93%

Fungal Seed Endophyte FZT214 Improves Dysphania ambrosioides Cd Tolerance Throughout Different Developmental Stages

Parmar

Sharma

et al. 2022

Front. Microbiol.

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Phytoremediation is a promising remediation method of heavy metal (HM)–contaminated soils. However, lower HM tolerance of metal accumulator inhibits its practical application and effects. The current study was aimed to illustrate the role of fungal seed endophyte (FZT214) in improving Dysphania ambrosioides Cd tolerance during different developmental stages under various Cd stresses (5, 15, 30 mg kg–1) by pot experiments. The results showed that FZT214 significantly (p < 0.05) improved the host plant’s growth at the flowering and fruiting stage in most of the treatment, while at the growing stage the increase was less (p > 0.05). The seed yield was also improved (p < 0.05) in the FZT214-inoculated plants (E+) and induced early flowering was observed. Moreover, the inoculation also positively affected total chlorophyll content, antioxidant process, and lipid peroxidation in most of the treatments throughout three developmental stages. Not all but in most cases, IAA and GA were more in E+ plants while JA was more in the E− plants (non-inoculated plants) during three developmental stages. The results suggested that the colonization of FZT214 to the D. ambrosioides might trigger multiple and comprehensive protective strategies against Cd stress, which mainly include activation of the dilution effects, induced biochemical changes to overcome damage from Cd toxicity, and alteration of the endogenous phytohormones. FZT214 can find competent application in the future to improve the growth of other crop plants.

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“…In our study, BS inoculation increased the GSH content of plants under NaCl stress, and PC inoculation increased the GSH content in plants under 0.1% NaCl; however, an increase in GSH content in the AC-inoculated plants was detected only under 0.2% and 0.3% NaCl. Similar results in which DSE could activate GSH metabolism in Zea mays under heavy metal stress have been reported [65]. These results indicated that, by regulating the accumulation of antioxidants and the Na + :K + balance, DSE can alleviate the adverse effects of NaCl stress on plants.…”

Section: Discussionsupporting

confidence: 86%

Effect of Dark Septate Endophytes on Plant Performance of Artemisia Ordosica and Associated Soil Microbial Community Under Salt Stress

Hou

et al. 2020

Preprint

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Background: Fungal endophytes can improve plant tolerance to abiotic stress, however, the role of these plant–fungal interactions in desert species ecology and their management implications remain unclear. This study aimed to assess whether dark septate endophytes (DSE) can shift the performance of Artemisia ordosica and associated soil microbial community under salt stress.Methods: We investigated the effects of three DSE (Alternaria chlamydosporigena [AC], Paraphoma chrysanthemicola [PC] and Bipolaris sorokiniana [BS]) isolated from desert habitats on plant morphology, physiology and rhizosphere soil microhabitat of Artemisia ordosica seedlings under different NaCl concentrations (0 %, 0.1 %, 0.2 %, and 0.3 %) in a growth chamber. Results: Three DSE strains could colonize the roots of A. ordosica, and the symbiotic response with host plants depended on DSE species and NaCl concentration. The greatest benefits associated with DSE occurred under 0.1 % NaCl. Specifically, AC improved root morphology, and increased total biomass and superoxide dismutase (SOD) activity; PC increased root morphology, root biomass, and glutathione (GSH) and indoleacetic acid (IAA) contents; and BS promoted SOD activity and GSH and IAA contents. DSE reduced the root Na+ content. Interestingly, BS promoted gram-positive (G +) and gram-negative (G −) bacteria under 0.1 % NaCl and the abundance of AM fungi under 0.2 % and 0.3 % NaCl. PC positively affected fungi, AM fungi, G − bacteria and actinomycetes under 0.2 % and 0.3 % NaCl, while AC increased the abundance of all examined microbes under 0.3 % NaCl. A structural equation modeling (SEM) demonstrated that DSE not only positively affects A. ordosica performance but also directly or indirectly impacts soil microbes by regulating the soil organic carbon (SOC), available phosphorus (AP), and alkaline nitrogen (AN) content.Conclusions: DSE isolated from A. ordosica enhanced the root development of host plants and altered the soil nutrient content and soil microbiota under different NaCl concentrations, possibly contributing to plant growth and ecological adaptability under saline environment. These results contribute to the understanding of the ecological function of DSE in desert ecosystems and may be used to promote vegetation restoration in salinized desert areas.

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Effects of a root-colonized dark septate endophyte on the glutathione metabolism in maize plants under cadmium stress

Cited by 30 publications

References 60 publications

Dark Septate Endophytes Improve the Growth and the Tolerance of Medicago sativa and Ammopiptanthus mongolicus Under Cadmium Stress

Dark Septate Endophytes Improve the Growth and the Tolerance of Medicago sativa and Ammopiptanthus mongolicus Under Cadmium Stress

Fungal Seed Endophyte FZT214 Improves Dysphania ambrosioides Cd Tolerance Throughout Different Developmental Stages

Effect of Dark Septate Endophytes on Plant Performance of Artemisia Ordosica and Associated Soil Microbial Community Under Salt Stress

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