Endophytic bacteria with antagonistic traits inhabit the wood tissues of grapevines from Tunisian vineyards

Rezgui, Awatef; Ghnaya-Chakroun, A. Ben; Vallance, Jessica; Bruez, Émilie; Hajlaoui, Mohamed Rabeh; Sadfi‐Zouaoui, Najla; Rey, Patrice

doi:10.1016/j.biocontrol.2016.04.005

Cited by 31 publications

(37 citation statements)

References 60 publications

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“…In fact, it has been reported that the population of actinobacteria decreases along the plant axis from 20% in the root, to 5% in the graft union, and just 2% in the cane samples (44). Second, the observed differences may be attributed to some extent to the different isolation culture media used, and even to the different molecular techniques used in the two works (43,44).…”

Section: Discussionmentioning

confidence: 99%

“…In 15-year-old plants, the population of endophytic actinobacteria was reduced to 5%, with only 2 strains detected, belonging to the Microbacterium and Curtobacterium genera. Also recently, Rezgui et al (43) analyzed the population of endophytic bacteria that inhabit the wood tissues from grapevines of Tunisian vineyards. Actinobacteria were hardly detected, and Curtobacterium was the only genus found.…”

Section: Discussionmentioning

confidence: 99%

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Use of Endophytic and Rhizosphere Actinobacteria from Grapevine Plants To Reduce Nursery Fungal Graft Infections That Lead to Young Grapevine Decline

Álvarez-Pérez

González-García

Cobos³

et al. 2017

Appl Environ Microbiol

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Endophytic and rhizosphere actinobacteria isolated from the root system of 1-year-old grafted Vitis vinifera plants were evaluated for their activities against fungi that cause grapevine trunk diseases. A total of 58 endophytic and 94 rhizosphere isolates were tested. Based on an in vitro bioassay, 15.5% of the endophytic isolates and 30.8% of the rhizosphere isolates exhibited antifungal activity against the fungal pathogen Diplodia seriata, whereas 13.8% of the endophytic isolates and 16.0% of the rhizosphere isolates showed antifungal activity against Dactylonectria macrodidyma (formerly Ilyonectria macrodidyma). The strains which showed the greatest in vitro efficacy against both pathogens were further analyzed for their ability to inhibit the growth of Phaeomoniella chlamydospora and Phaeoacremonium minimum (formerly Phaeoacremonium aleophilum). Based on their antifungal activity, three rhizosphere isolates and three endophytic isolates were applied on grafts in an open-root field nursery in a 3-year trial. The field trial led to the identification of one endophytic strain, Streptomyces sp. VV/E1, and two rhizosphere isolates, Streptomyces sp. VV/R1 and Streptomyces sp. VV/R4, which significantly reduced the infection rates produced by the fungal pathogens Dactylonectria sp., Ilyonectria sp., P. chlamydospora, and P. minimum, all of which cause young grapevine decline. The VV/R1 and VV/R4 isolates also significantly reduced the mortality level of grafted plants in the nursery. This study shows that certain actinobacteria could represent a promising new tool for controlling fungal trunk pathogens that infect grapevine plants through the root system in nurseries. IMPORTANCE Grapevine trunk diseases are a major threat to the wine and grape industry worldwide. They cause a significant reduction in yields as well as in grape quality, and they can even cause plant death. Trunk diseases are caused by fungal pathogens that enter through pruning wounds and/or the root system. Although different strategies have recently been developed to protect pruning wounds using antifungal compounds (natural or synthetic) or biocontrol agents, no tools are yet available for controlling soil pathogens that infect plants through their root system. This study shows that different actinobacterial isolates, when applied to grafts in a nursery, can significantly reduce the infection rate caused by fungal pathogens that enter through the root system. This is a new, promising, and green alternative for preventing the decline of young grapevines in nurseries and vineyards.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Use of Endophytic and Rhizosphere Actinobacteria from Grapevine Plants To Reduce Nursery Fungal Graft Infections That Lead to Young Grapevine Decline

Álvarez-Pérez

González-García

Cobos³

et al. 2017

Appl Environ Microbiol

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“…Similar results were reported by Santos et al (2016) which had inhibition of mycelial growth from 20.9% to 50.6% in D. macrodidyma, using commercial products containing B. subtilis (Rizos and Rizolyptus). Another study evaluating the action of 19 bacterial strains belonging to the genera Pantoea, Pseudomonas, Curtobacterium and Bacillus on the growth of fungi causing stem disease in vines (Lasidiodiplodia theobromae, Neofusicoccum parvum and Schizophyllum commune) showed inhibition varying from 2.5% to 81.5%, with a mean of 24.9% in the paired culture (Rezgui et al 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Microorganisms of the former group usually have a broad spectrum of action, being the production of toxic substances more effective than other mechanisms involved in inhibition of pathogen growth (Santos et al 2015). Studies have shown the efficacy of B. subtilis in the control of vine fungal pathogen including Eutypa lata (Ferreira et al 1991;Kotze et al 2011), Botrytis cinerea and Colletotrichum gloeosporioides (Furuya et al 2011;Boubakri et al 2015), Phaeomoniella chlamydospora, Phomopsis viticola, Diplodia seriata, Lasiodiplodia theobromae, Neofusicoccum australe and N. parvum (Kotze et al 2011;Rezgui et al 2016), Plasmopara viticola (Furuya et al 2011;Boubakri et al 2015) and Dactylonectria macrodidyma (Santos et al 2016). Furthermore, B. subtilis works as a plant growth-promoting rhizobacterium that stimulates the growth and development of plants, through the bioavailability of nutrients and the stimulus of hormonal biosynthesis, increasing the yield, reducing biotic and abiotic stresses (Compant et al 2010;Kejela et al 2017).…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Biocontrol of black foot disease on grapevine rootstocks using Bacillus subtilis strain F62

Russi

Almança

Grohs

et al. 2020

Trop. plant pathol.

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Decline and death of young vines is a worldwide problem for viticulture that may lead to economic loss. Fungal pathogens have been associated with trunk and root diseases, including the black foot disease that affects the performance of the vineyards. Fungicides have not worked efficiently to control the disease and alternative methods should be investigated. We evaluated the potential of Bacillus subtilis strain F62 for controlling the disease caused by different strains in grapevine rootstocks 1103P (Vitis berlandieri × V. rupestris) and SO4 (V. berlandieri × V. riparia). The in vitro antagonism of B. subtilis F62 was evaluated on mycelial growth, by diffusible and volatile compounds, and conidia germination, by bacterial suspension and cell-free filtrate. In the in vivo assay, cuttings and micropropagated rootstocks were submitted to four different treatments: control, Bac (B. subtilis inoculation), Pat (pathogen inoculation) and Bac + Pat. According to our results, the bioagent was able to inhibit the mycelial growth of all the three fungal isolates by diffusible compounds and conidial germination by bacterial suspension and cell-free filtrate. In the in vivo assay, cuttings of SO4 treated with B. subtilis F62 showed higher shoot nodes and length of primary shoot, while cuttings of 1103P had a longer primary shoot. In micropropagated plants, B. subtilis F62 promoted plant growth in both rootstocks and reduced the frequency of D. macrodidyma re-isolation to 24.6% in SO4 and 29.5% in 1103P. The results demonstrated the potential of B. subtilis F62 on plant growth promotion and in the biocontrol of black foot disease on micropropagated plants and cuttings of grapevine rootstocks 1103P and SO4.

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“…For example, using SSCP of bacterial 16S rDNA, soil salinity has been shown to increase the diversity of bacteria in sugarcane ( Saccharum officinarum L.) rhizospheres (Lamizadeh et al., ), whereas maize ( Zea mays L.) rhizobacterial populations have been shown to significantly vary by soil salinity levels, organic carbon, calcium, and geography (Castellanos et al., ). SSCP was used to profile bacterial populations in healthy and necrotic wood tissues of mature Tunisian grapevines ( Vitis vinifera L.), aiding in the discovery and isolation of 19 unique endophytes with biocontrol potential (Rezgui et al., ). Similarly, in an intriguing methods paper, Zachow et al.…”

Section: Comparing Dna‐based Methods For Studying Plant Microbiomes (mentioning

confidence: 99%

Botanical microbiomes on the cheap: Inexpensive molecular fingerprinting methods to study plant‐associated communities of bacteria and fungi

Johnston‐Monje

Mejía

2020

Appl Plant Sci

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High‐throughput sequencing technologies have revolutionized the study of plant‐associated microbial populations, but they are relatively expensive. Molecular fingerprinting techniques are more affordable, yet yield considerably less information about the microbial community. Does this mean they are no longer useful for plant microbiome research? In this paper, we review the past 10 years of studies on plant‐associated microbiomes using molecular fingerprinting methodologies, including single‐strand conformation polymorphism (SSCP), denaturing gradient gel electrophoresis (DGGE), amplicon length heterogeneity PCR (LH‐PCR), ribosomal intergenic spacer analysis (RISA) and automated ribosomal intergenic spacer analysis (ARISA), and terminal restriction fragment length polymorphism (TRFLP). We also present data juxtaposing results from TRFLP methods with those generated using Illumina sequencing in the comparison of rhizobacterial populations of Brazilian maize and fungal surveys in Canadian tomato roots. In both cases, the TRFLP approach yielded the desired results at a level of resolution comparable to that of the MiSeq method, but at a fraction of the cost. Community fingerprinting methods (especially TRFLP) remain relevant for the identification of dominant microbes in a population, the observation of shifts in plant microbiome community diversity, and for screening samples before their use in more sensitive and expensive approaches.

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Endophytic bacteria with antagonistic traits inhabit the wood tissues of grapevines from Tunisian vineyards

Cited by 31 publications

References 60 publications

Use of Endophytic and Rhizosphere Actinobacteria from Grapevine Plants To Reduce Nursery Fungal Graft Infections That Lead to Young Grapevine Decline

Use of Endophytic and Rhizosphere Actinobacteria from Grapevine Plants To Reduce Nursery Fungal Graft Infections That Lead to Young Grapevine Decline

Biocontrol of black foot disease on grapevine rootstocks using Bacillus subtilis strain F62

Botanical microbiomes on the cheap: Inexpensive molecular fingerprinting methods to study plant‐associated communities of bacteria and fungi

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