Endophytic bacteria isolated from higher plant in Aceh, Indonesia, and their chemical compounds activity against Fusarium oxysporum f. sp. lycopersici

Abstract

Background Endophytic bacteria are an association between bacteria and plant tissue that could play a role as a biocontrol agent. Main body Endophytic bacteria were isolated from several high root plants in Aceh, Indonesia. This study aimed to detect the chemical compounds of the potential endophytic bacteria as a biocontrol agent against Fusarium oxysporum f. sp. lycopersici (FOL). There were 198 endophytic bacterial isolates detected in roots of 9 higher plant. The hypersensitive reaction showed that 193 isolated endophytic bacteria were non-pathogenic. There were 13 isolated endophytic bacteria that worked to inhibit FOL between 50.0 and 89.2%; such endophytic bacteria were isolated from Solanum lycopersicum L., Psidium guajava L., Dendrocalamus asper (Schult with f.) Backer ex Heyne, Pinus merkusii L., Theobroma cacao L., and Albizia chinensis L. Molecular identification using 16S rRNA gene sequence confirmed that the endophytic bacteria were derived from species Pseudomonas aeruginosa, P. mosselii, Arthrobacter sp., Bacillus cereus, B. thuringiensis, and Serratia marcescens. P. aeruginosa that showed the highest inhibition was analyzed using GC-MS analysis. The analysis identified that antibiotics as Pyrrolo [1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)- was produced by P. aeruginosa succeeded in suppressing FOL. Conclusion The study recommends the species P. aeruginosa, as effective endophytic bacteria for the control of FOL pathogen.

Application of Trichoderma harzianum and Bacillus thuringiensis in increasing plant growth of Aceh patchouli (Pogostemon cablin Benth)

“…B. thuringiensis also can produce secondary metabolites that have a beneficial role for plants. Secondary metabolites produced by B. thuringiensis are hydrolytic enzymes (cellulase, protease, and chitinase), hydrogen cyanide (HCN), indole 3-acetic acid (IAA), 1-aminocyclopropane 1-carboxylic acid (ACC) deaminase, and siderophores (8). The ability of B. thuringiensis as PGPR enhances plant growth through several actions, both direct and indirect.…”

Evaluating the efficacy of Trichoderma harzianum and Bacillus thuringiensis on induce the plant growth and resistance of local variety patchouli under covered and uncovered seedlings methods

“…The bacteria Arthrobacter sp. and B. thuringiensis produced IAA of 0.099 and 0.101, to increase the height of tomato plants [10]. B. thuringiensis was able to increase the height of chili plants up to 106.33 cm [13] giving Trichoderma sp.…”

“…The application of Trichoderma harzianum in increasing plant growth and decreasing inhibition of plant pathogens, such as fungi, bacteria and nematode have been determining by several reports [9]. Other side the endophytic bacteria have been isolated from root of higher plant in Aceh and identified as B. thuringiensis [10], this bacterium could inhibit nematode pathogen growth and fusarium fungi by partied, enzyme and mycotoxin mechanism [10,11].…”

Antifungal Potential of Bacillus subtilis subsp. inaquosorum RLS76 for Management of Fusarium Wilt Disease of Bt- Cotton

“…There is limited literature on controlling Fusarium wilt in Bt-cotton using microorganisms. Despite the work of many researchers in the field of biological control, previous studies have mainly explored the use of endophytic bacteria [16][17] and Trichoderma sp. [18][19] for managing Fusarium wilt in cotton.…”