Endophytes from medicinal plants and their potential for producing indole acetic acid, improving seed germination and mitigating oxidative stress

Abstract

Abstract:Medicinal plants have been used by marginal communities to treat various ailments. However, the potential of endophytes within these bio-prospective medicinal plants remains unknown. The present study elucidates the endophytic diversity of medicinal plants (Caralluma acutangula, Rhazya stricta, and Moringa peregrina) and the endophyte role in seed growth and oxidative stress. Various organs of medicinal plants yielded ten endophytes, which were identified as Phoma sp. (6 isolates), Alternaria sp. (2), Bipolaris sp. (1), and Cladosporium sp. (1) based on 18S rDNA sequencing and phylogenetic analysis. The culture filtrates (CFs; 25%, 50%, and 100% concentrations) from these endophytes were tested against the growth of normal and dwarf mutant rice lines. Endophytic CF exhibited dose-dependent growth stimulation and suppression effects. CF (100%) of Phoma sp. significantly increased rice seed germination and growth compared to controls and other endophytes. This growth-promoting effect was due to the presence of indole acetic acid in endophytic CF. The gas chromatography/mass spectrometry (GC/MS) analysis showed the highest indole acetic acid content ((54.31±0.21) µmol/L) in Bipolaris sp. In addition, the isolate of Bipolaris sp. exhibited significantly higher radical scavenging and anti-lipid peroxidation activity than the other isolates. Bipolaris sp. and Phoma sp. also exhibited significantly higher flavonoid and phenolic contents. The medicinal plants exhibited the presence of bio-prospective endophytic strains, which could be used for the improvement of crop growth and the mitigation of oxidative stresses.

Tuber indicum shapes the microbial communities of ectomycorhizosphere soil and ectomycorrhizae of an indigenous tree (Pinus armandii)

“…Endophytes and soil microbes play an important role in the growth and development of the plant host [31–34]. They can participate in the metabolic processes of the host, produce biological molecules that have growth-promoting or antibacterial activities, and ultimately affect the yield and quality of the host [35–38].…”

TRIAGEM DE POTENCIALIDADES BIOTECNÓLOGICAS DE BACTÉRIAS ENDOFÍTICAS ISOLADAS DE FOLHAS Gossypium hirsutum L.

“…A produção dessa auxina é uma característica comum em bactérias endofíticas e tem sido observada em diversas espécies vegetais tais como milho (SILVA et al, 2016), batata (OGATA-GUTIÉRREZ et al, 2016) e diversas plantas medicinais (KHAN et al, 2017). Embora os endofíticos sejam capazes de produzir um grande número de fitohormônios, as auxinas e, em especial, o ácido-3-indol ácetico (AIA) são os mais comumente gerados.…”

Aspergillus niger CSR3 regulates plant endogenous hormones and secondary metabolites by producing gibberellins and indoleacetic acid

“…Production of phytohormones is a key attribute enabling endophytes to promote plant growth by improving the ability of the host to tolerate and overcome different abiotic and biotic environmental stresses (Ma et al 2016;Hamayun et al 2017;Khan et al 2017;Sandhya et al 2017). Therefore, to determine the phytohormones i.e.…”