Aspergillus niger CSR3 regulates plant endogenous hormones and secondary metabolites by producing gibberellins and indoleacetic acid

Abstract

In this study, an endophytic fungus, Aspergillus niger CSR3, was isolated from Cannabis sativa. The culture filtrate (CF) was initially screened for growth-promoting activities such as the presence of siderophores, phosphate solubilization, and the production of indole acetic acid (IAA) and gibberellins and was further assayed for its ability to promote the growth of mutant waito-C rice. Nearly all plant growth attributes examined (root-shoot length, biomass, and chlorophyll content) were significantly enhanced by treatment with CSR3. This growth promotion action was due to the presence of various types of gibberellins (GAs) and IAA in the endophyte CF. Moreover, the presence of GA pathway genes (P50-1, P450-3, P450-4, ggs2, and des) was confirmed by means of semi-quantitative RT-PCR. Finally, the application of CSR3 spore suspension with uniconazole and yucasin on maize seedlings revealed that, similar to exogenous IAA and GA 3 , CSR3 has the potential to alleviate the inhibitory effect of these inhibitors.

IAA and flavonoids modulates the association between maize roots and phytostimulant endophytic Aspergillus fumigatus greenish

“…Internal tissues of the sterilized segments were then exposed by firmly against glass slide and the injured segments were kept on Hagam agar (0.5% glucose, 0.05% KH 2-PO 4 , 0.05% MgSO 4 •7H 2 O, 0.05% NH 4 Cl, 0.1% FeCl 3 , 80 ppm streptomycin and 1.5% agar; pH 5.6 ± 0.2) at the rate of 5 pieces/dish. Imprints of the surface sterilized explants on Hegam medium were used to test the efficiency of surface sterilization protocol (Lubna et al 2018). Control plates received surface sterilized leaves segments that were not injured.…”

WITHDRAWN: Plant growth promoting Bipolaris sp. CSL-1 mitigate salinity stress in soybean via altering endogenous phytohormonal level, antioxidants and genes expression

“…Phytohormones producing plant growth promoting endophytic fungi produce different plant hormones, i.e. gibberellins, indole-3-acetic acid and organic compounds that help the plants to tolerate or avoid abiotic stress including salinity stress [35,36]. Previously [37] and [38] reported that GAs producing endophytic fungi Phoma herbarum and Penicillium sp mitigate salinity stress and enhance plant growth in soybean and cucumber.…”

“…Isolation, screening and identi cation Several plant growths promoting endophytic fungi (previously reported [17,36,55,56] were deposited to gene bank in the crop physiology lab, school of applied biosciences, Kyungpook National University. These fungi were screened for NaCl stress resistance by using ve concentration of NaCl (0mM, 50 mM, 100 mM, 150 mM, 200 mM and 250 mM) and 0.1% of culture aliquot was inoculated into 100 ml of sterilized czapek media and incubated in a shaking incubator at 30°C.…”

The Multiple Facets of Plant–Fungal Interactions Revealed Through Plant and Fungal Secretomics

“…The endophytic fungus A. niger CSR3 produces IAA and GAs to promote growth of the GA-deficient rice mutant Waito-C (Lubna Asaf et al, 2018). Multifunctional plant growth-promoting fungi (PGPF) include the fungal genera Aspergillus, Fusarium, Penicillium, Piriformospora, Phoma, and Trichoderma.…”