Endonucleases

Sugiyama, Masaru; Ito, Jun; Aoyagi, Shigemi; Fukuda, Hiroo

doi:10.1007/978-94-010-0934-8_11

Cited by 30 publications

(46 citation statements)

References 46 publications

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“…BEN1 is a Zn 2+ -dependent endonuclease cDNA from barley and has been associated with PCD [1,27]. Its homology was analyzed with the NCBI Genbank database based on the nucleotide sequences of BEN1.…”

Section: Acta Biologica Hungarica 66 2015mentioning

confidence: 99%

“…Furthermore, Zn 2+ -dependent endonuclease cDNAs (Bnuc1) isolated from salt-stressed or senescent leaves in barley were detected [20]. The S1-type and Zn 2+ -dependent DNase groups have been associated with PCD [1,27,36]. In addition, a previous study revealed an endonuclease which is responsible for DNA laddering by the D-mannose system in Arabidopsis roots and maize (Zea mays) [26].…”

Section: Introductionmentioning

confidence: 99%

“…Nuclear endonuclease is essential for DNA fragmentation and other morphologic changes of apoptotic nuclei [13,22,27]. In addition, nuclease induction is strongly associated with plant PCD processes [7,27]. For example, several nucleases have been identified to be responsible for hypersensitive response to cell death signal [18][19].…”

Section: Introductionmentioning

confidence: 99%

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Localization of BEN1-LIKE protein and nuclear degradation during development of metaphloem sieve elements inTriticum aestivumL.

Cai¹,

Zhang²,

Zhou³

et al. 2015

Acta Biologica Hungarica

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Metaphloem sieve elements (MSEs) in the developing caryopsis of Triticum aestivum L. undergo a unique type of programmed cell death (PCD); cell organelles gradually degrade with the MSE differentiation while mature sieve elements keep active. This study focuses on locating BEN1-LIKE protein and nuclear degradation in differentiating MSEs of wheat. Transmission electron microscopy (TEM) showed that nuclei degraded in MSE development. First, the degradation started at 2-3 days after flowering (DAF). The degraded fragments were then swallowed by phagocytic vacuoles at 4 DAF. Finally, nuclei almost completely degraded at 5 DAF. We measured the BEN1-LIKE protein expression in differentiating MSEs. In situ hybridization showed that BEN1-LIKE mRNA was a more obvious hybridization signal at 3-4 DAF at the microscopic level. Immuno-electron microscopy further revealed that BEN1-LIKE protein was mainly localized in MSE nuclei. Furthermore, MSE differentiation was tested using a TSQ Zn 2+ fluorescence probe which showed that the dynamic change of Zn 2+ accumulation was similar to BEN1-LIKE protein expression. These results suggest that nucleus degradation in wheat MSEs is associated with BEN1-LIKE protein and that the expression of this protein may be regulated by Zn 2+ accumulation variation.

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Section: Acta Biologica Hungarica 66 2015mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Localization of BEN1-LIKE protein and nuclear degradation during development of metaphloem sieve elements inTriticum aestivumL.

Cai¹,

Zhang²,

Zhou³

et al. 2015

Acta Biologica Hungarica

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“…CMN1 nuclease was active in extracts collected in May, and CMN2 during the whole vegetation period (from May until October). Both proteins exhibit the highest activity in Ca 2+ buffer, pH=8.0, so they belong to the Ca 2+ -dependent nucleases class [4]. The activity of both nucleases is very high and changes during growth and development of the plant.…”

Section: Introductionmentioning

confidence: 99%

Nucleases isolated from Chelidonium majus L. milky sap can induce apoptosis in human cervical carcinoma HeLa cells but not in Chinese Hamster Ovary CHO cells.

Nawrot

Wołuń-Cholewa²,

Goździcka-Józefiak³

2008

Folia Histochem Cytobiol.

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Milky sap isolated from Chelidonium majus L. (Greater Celandine) serves as a rich source of various biologically active substances such as alkaloids, flavonoids and phenolic acids. Previous research showed that the activity of Ch. majus milky sap may depend also on the presence of biologically active proteins. The goal of this study was to evaluate the biological effect of two nucleases isolated from Ch. majus milky sap, CMN1 of 20 kDa and CMN2 of 36 kDa, on HeLa and CHO tumour cell lines. Both studied nucleases together with other proteins in the sap of the plant are involved in stress and defence reactions against different pathogens. After 48 h incubation of CMN1 and CMN2 only with HeLa cells, the dependence between the number of apoptotic lesions and the concentration of applied nuclease was observed. The highest proapoptotic activity was induced by 13.3 ng/ml concentration of CMN2 collected in May (62 ± 3% HeLa cells were apoptotic). Moreover, the proportion of necrotic cells in all concentrations of the nucleases and both cell lines was relatively low (1-8 ± 0.5%). In summary, results of this study show that purified nucleases CMN1 and CMN2 isolated from Ch. majus milky sap exhibit apoptotic activity in HeLa tumour cell line, but not in CHO cells, without inflammatory reaction.

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“…2,4 They hydrolyze RNA, single-and double-stranded DNA and are similar to some extent to fungal S1 and P1 nucleases. 5 Ca 2+ -dependent plant endonucleases are inhibited by Zn 2+ . They can often be activated by Mg 2+ and Mn 2+ as well.…”

Section: Introductionmentioning

confidence: 99%

Wheat Endonuclease WEN1 Dependent on S-Adenosyl-L-Methionine and Sensitive to DNA Methylation Status

Fedoreyeva

Sobolev²,

Vanyushin³

2007

Epigenetics

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Endonucleases

Cited by 30 publications

References 46 publications

Localization of BEN1-LIKE protein and nuclear degradation during development of metaphloem sieve elements inTriticum aestivumL.

Localization of BEN1-LIKE protein and nuclear degradation during development of metaphloem sieve elements inTriticum aestivumL.

Nucleases isolated from Chelidonium majus L. milky sap can induce apoptosis in human cervical carcinoma HeLa cells but not in Chinese Hamster Ovary CHO cells.

Wheat Endonuclease WEN1 Dependent on S-Adenosyl-L-Methionine and Sensitive to DNA Methylation Status

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