Endometritis: new time, new concepts

Kitaya, Kotaro; Takeuchi, Takumi; Mizuta, Shimpei; Matsubayashi, Hidehiko; Ishikawa, Tomomoto

doi:10.1016/j.fertnstert.2018.04.012

Cited by 211 publications

(222 citation statements)

References 79 publications

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“…Chronic endometritis (CE), the best‐studied subclinical inflammation of the endometrial lining, is mainly caused by infection with common bacteria such as Enterobacteria, Streptococcus species, and Enterococcus faecalis , as well as Chlamydia , Mycoplasma , and Ureaplasma species, although yeast can be also involved, to the detriment of the protective LD microbiota . The reported prevalence of this disease in reproductive‐age women varies from 8% to 72%, and it is associated with repeated implantation failure and recurrent pregnancy loss . However, because it is often asymptomatic, it is rarely suspected, diagnosed, and treated.…”

Section: Role Of Bacteria In Women's Healthmentioning

confidence: 99%

“…One of the challenges of CE management today is the lack of universally standard criteria for the diagnosis of the disease (ie, number of plasma cells identified per area of screened tissue sections). While the current gold‐standard method relies on histopathological identification of plasmacyte infiltrations in the endometrial stroma coupled to CD138 immunohistochemistry, other authors promote hysteroscopic examination of the uterine cavity or the classical culture of endometrial specimens . However, many confounding factors affect the assessment of CE: the method of analysis, the criteria used to discriminate positive and negative samples, the phase of the cycle in which the endometrium is evaluated (ie, the estimated prevalence of CE is approximately 50% higher in endometrial samples collected in the proliferative vs the secretory phase), or even the reagents used in the laboratory developing the test .…”

Section: Role Of Bacteria In Women's Healthmentioning

confidence: 99%

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Deciphering the effect of reproductive tract microbiota on human reproduction

Moreno

Simón

2018

Reprod Medicine & Biology

107

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BackgroundThe female reproductive tract contains an active microbiome comprising mainly bacteria from the Lactobacillus genus, which is associated with a healthy microbiome state. However, spatio‐temporal fluctuations of this microbiome that occur in response to internal and external factors may impact the physiology of the reproductive tract organs and even lead to pathological states.MethodsCurrent literature covering the reproductive tract microbiome is summarized and contextualized in this review.Main findingsThis review presents the current knowledge about the bacterial composition of the lower and upper reproductive tract as well as the impact of the microbiota on women's health and reproduction. We place special focus on the impact of the endometrial microbiome in infertility and assisted reproductive technologies.ConclusionThe assessment of the reproductive tract microbiome adds a new microbiological perspective to human reproduction, pregnancy, and onset of new life, highlighting the importance of considering the evaluation of microbial communities to improve personalized care in reproductive medicine and women's health.

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Section: Role Of Bacteria In Women's Healthmentioning

confidence: 99%

Section: Role Of Bacteria In Women's Healthmentioning

confidence: 99%

Deciphering the effect of reproductive tract microbiota on human reproduction

Moreno

Simón

2018

Reprod Medicine & Biology

107

View full text Add to dashboard Cite

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“…69,70 Moreover, recent studies have demonstrated that G-CSF treatment in women with recurrent IVF failure improved implantation and pregnancy rates. 83 Furthermore, use of these in vivo and in vitro models provides an avenue to identify and test targets for therapeutic intervention, ultimately improving fertility. 72,73 Primary human endometrial stromal cells decidualized in the presence of LPS expressed significantly lower transcript levels of decidual biomarkersIGFBP1 and PRL compared to those decidualized without LPS ( Figure 5A).…”

Section: Identifying the Molecular Changes That Accompany Lps-inducmentioning

confidence: 99%

“…As chronic endometritis is associated with poor reproductive outcomes, these new models are directly relevant to enhancing our understanding of how bacterial endotoxins alter the uterine environment. 83 Furthermore, use of these in vivo and in vitro models provides an avenue to identify and test targets for therapeutic intervention, ultimately improving fertility. 84…”

Section: Characterizing Cytokine and Chemokine Release In Primary Hmentioning

confidence: 99%

New models of lipopolysaccharide‐induced implantation loss reveal insights into the inflammatory response

Moustafa

Joseph

Taylor

et al. 2019

American J Rep Immunol

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Problem Chronic endometritis, inflammation of the uterizzvvne lining caused by common gram‐negative bacterial strains or mycoplasma, has been associated with unexplained implantation failure and infertility. However, limited models of bacteria‐induced implantation loss exist to study the molecular changes that occur in vivo. The goal of this study was to provide a new resource to study the process of bacteria‐induced inflammation and implantation loss utilizing common experimental models: C57Bl/6 mice and primary human endometrial stromal cells. Method of study Prior to implantation, mated C57Bl/6 females were administered vehicle (saline) or gram‐negative bacterial lipopolysaccharide (LPS) at a range of concentrations by intraperitoneal injection. Implantation sites were counted, and uteri were harvested to evaluate the molecular changes that accompany LPS‐mediated implantation loss. Primary human endometrial stromal cells were decidualized in vitro in the presence and absence of LPS. Total RNA and conditioned media were harvested to evaluate the expression of known decidualization‐associated genes and various cytokines and chemokines. Results Lipopolysaccharide treatment resulted in fewer implantation sites in mice, decreased expression of decidualization‐associated genes, and altered expression and release of cytokines and chemokines. Immunohistological analysis of the uterus from LPS‐exposed mice demonstrated increased apoptosis and decreased proliferation during decidualization. Conclusion Lipopolysaccharide exposure disrupted implantation and decidualization in mice and human endometrial stromal cells. This model could be used to study the pathophysiology of implantation failure in patients with chronic endometritis or to test potential therapeutic interventions.

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“…In recent years, with the development of high-throughput sequencing technology of 16S rDNA gene, which is possible to analyze the diversity of bacterial community in different ecological niches. Growing evidence suggests that the intestinal micro ora affects many important physiological functions of the host, such as immune system activation, metabolism, epithelial cell proliferation and anti-infection [4].…”

Section: Introductionmentioning

confidence: 99%

Effect of intestinal flora on the formation of endometritis of sow

Zhang¹,

Wang²,

Dai³

et al. 2020

Preprint

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Background Aberration of birth canal microbiota is one of the most important factors in the etiology of sow endomentritis. Nevertheless, reports about the structure and composition of birth canal microbiota in endomentritis sow and their relationship with intestinal microbes is limited. Therefore, understanding the relationship between birth canal microbiota and intestinal microbiota of the host has become exceedingly crucial. Results In this study, 4 healthy and 4 endomentritis sows were selected basing on whether the sow had endometritis or not in a farm of China. The microflora of their birth canal secretions and fresh feces were analyzed via sequencing the V3 + V4 region of bacterial 16S rDNA gene. The results showed that the significant difference between endometritis and healthy sows birth canal flora in the composition and abundance. Sow endomentritis was associated with increasing in the relative abundance of Porphyromonas, Clostridium_sensu_stricto_1, Streptococcus, Ezakiella, Fusobacterium, Actinobacillus, Bacteroides, and Prevotella as well as Anaerococcus. On the contrary, the majority of beneficial bacteria that belonging to Firmicutes phylum (e.g., Lactobacillus and Enterococcus ) declined in endomentritis sow. The increased relative abundance of Porphyromonas in the vaginal secretions might correlate with the decrease of Lactobacillusin the feces of endometritis sows. Moreover, the experimental result also found that some intestinal bacteria (such as Escherichia-Shigella and Bacteroides) may be bound up with the onset of sow endometritis. Conclusion Sow endometritis is closely related to the microbiota of birth canal, and that some intestinal bacteria may promote the onset of endometritis. The above results can supply a theoretical basis to research the pathogenesis of endometritis and the microbiota of sow's birth canal and gut.

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Endometritis: new time, new concepts

Cited by 211 publications

References 79 publications

Deciphering the effect of reproductive tract microbiota on human reproduction

Deciphering the effect of reproductive tract microbiota on human reproduction

New models of lipopolysaccharide‐induced implantation loss reveal insights into the inflammatory response

Effect of intestinal flora on the formation of endometritis of sow

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