Endometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry

Abstract

Obtaining fast screening information on molecular composition of a tissue sample is of great importance for a disease biomarkers search and for online surgery control. In this study, high resolution mass spectrometry analysis of eutopic and ectopic endometrium tissues (90 samples) is done using direct tissue spray mass spectrometry in both positive and negative ion modes. The most abundant peaks in the both ion modes are those corresponding to lipids. Species of three lipid classes are observed, phosphatidylcholines (PC), sphingomyelins (SM) and phosphoethanolamines (PE). Direct tissue analysis gives mainly information on PC and SM lipids (29 species) in positive ion mode and PC, SM and PE lipids (50 species) in negative ion mode which gives complementary data for endometriosis foci differentiation. The biggest differences were found for phospholipids with polyunsaturated acyls and alkils. Although, tissue spray shows itself as appropriate tool for tissue investigation, caution should be paid to the interpretation of mass spectra because of their higher complexity with more possible adducts formation and multiple interferences must be taken into account. The present work extends the application of direct tissue analysis for the rapid differentiation between endometriotic tissues of different foci.

Discovery of Phosphatidic Acid, Phosphatidylcholine, and Phosphatidylserine as Biomarkers for Early Diagnosis of Endometriosis

“…Furthermore, MTBE is noncorrosive and chemically stable without forming peroxides during storage, which presents no danger to degrade labile lipids (Matyash et al, 2008 ). Previous studies reported alterations of lipid profile in serum, peritoneal fluid, follicular fluid, and endometrium of endometriosis patients (Vouk et al, 2012 , 2016 ; Cordeiro et al, 2015 ; Chagovets et al, 2017 ). However, it is has not been investigated the alteration of lipid metabolism in endometrium.…”

Validation of Breast Cancer Margins by Tissue Spray Mass Spectrometry

“…The data on a sample molecular composition are obtained by tissue spray mass spectrometry [24][25][26]. The positive and negative ionization modes are used to provide complementary information and to find out the most informative method for a breast tissue classification.…”

“…These peaks correspond to different lipid species. Lipid peaks are typically dominated in tissue spray mass spectra [24][25][26], therefore, R-scripts generating theoretical lipid masses are developed to identify the obtained spectra. The possibility of ion formation by protons, sodium cation or potassium cation attachment in the positive ion mode is considered [29][30][31][32].…”

“…In our laboratory we are developing tissue spray mass spectrometry for the study of lipid markers of endometriosis tissues, lung cancer, and brain tumors [24][25][26][27][28][29][30][31]. The complete obviation of sample preparation and chromatographic separation stages allows rapid analysis without significant loss of chemical information [32].…”

The Lipid-related Effects of Resveratrol on Human Ectopic Endometrial Stromal Cells and a Rat Model of Endometriosis

“…In such studies, elevated levels of SM, PC and TG were identi ed in serum samples of OMA patients (15). Signi cant alterations in SM, PC, TG and PE between the eutopic and ectopic endometrium were also noted in patients with EMs (16,17). As a direct in ltration environment for ectopic endometrium, peritoneal uid in patients with EMs was found to have a decreased PC level (18,19).…”