The Lipid-related Effects of Resveratrol on Human Ectopic Endometrial Stromal Cells and a Rat Model of Endometriosis

Abstract

Background: Endometriosis is a complex disease in the field of gynecology that has certain limitations for its interim treatment. Resveratrol has been recently used for the treatment of endometriosis in experimental and clinical studies, but its molecular mechanism remaines elusive. Results: In this study, based on a case-control study, we identified that a decreased BMI and altered lipid profiles were associated with endometriosis patients. We applied resveratrol treatment on human ectopic endometrial stromal cells (HEcESCs) and a rat model of endometriosis. Lipidomics analysis showed that resveratrol altered lipid profiles in HEcESCs, with the sphingolipids Cer and SM increased significantly, while FA and most phospholipids were significantly reduced. Pathway enrichment analysis showed that several lipid-associated signaling pathways could be targeted by resveratrol. Our experiments in a rat model showed that resveratrol reduced the lesion and rectified lipid profiles in rats with endometriosis. In addition, resveratrol treatment significantly increased the expression of PPARα in lesion tissues of model rats and HEcESCs of EMs patients.Conclusion: Our data reveal that the development of EMs is closely related to lipid metabolism, and resveratrol may play a therapeutic role by targeting the lipid metabolism of ectopic endometrial stromal cells in endometrosis. Our study provides valuable insights for understanding the pathogenesis and clinical treatment of endometriosis.

Transcriptome-Based Analysis Reveals Therapeutic Effects of Resveratrol on Endometriosis in aRat Model

“…In our previous study, we analyzed the effects of resveratrol treatment on human ectopic endometrial stromal cells. 49 Here, lipidomics analysis showed that resveratrol altered lipid profiles and resulted in changes in glycerophospholipid metabolism pathways and in the activation of insulin resistance. To further confirm, we performed examinations of GTT and ITT, adipocyte histology and flow cytometry of the peritoneal fluid in the EMs rats (Figure4A).…”