Endometrial transcripts of proinflammatory cytokine and enzymes in prostaglandin synthesis are upregulated in the bitches with atrophic pyometra

Singh, Laishram Kipjen; Patra, Manas Kumar; Mishra, G K; Singh, Vidya; Upmanyu, Vikramaditya; Saxena, Abhishek; Singh, Sanjay K.; Das, G. C.; Kumar, Harendra; Krishnaswamy, Narayanan

doi:10.1016/j.vetimm.2018.10.010

Cited by 12 publications

(16 citation statements)

References 29 publications

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“…A significant up-regulation of endometrial cylooxygenase-2 gene is reported in bitches with pyometra (Silva et al 2009). Recently, we have reported upregulation of IL-6, IL-8, COX-2 and PGFS in the pyometra bitches associated with endometrial atrophy (Singh et al 2018). From this study, it is concluded that serum PGFM is elevated in SIRS+ canine pyometra and can aid in disease monitoring along with routine serum biochemistry.…”

Section: Resultssupporting

confidence: 51%

Effect of systemic inflammatory response syndrome (SIRS) on prostaglandin metabolite and oxidative stress in canine pyometra

Singh

Patra

Mishra

et al. 2020

Indian J of Anim Sci

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Pyometra, either open or closed cervix, inevitably progresses to systemic inflammatory response syndrome (SIRS), if ovariohysterectomy is not done timely. The aim of the study was to investigate the effect of pyometra led SIRS on certain serum biochemical and prostaglandin metabolite, 13,14-dihydro-15-keto PGF2 alpha (PGFM) and oxidative stress indices in the canine. The pyometra positive bitches were categorized as SIRS+ (n=29) and SIRS- (n =16) based on temperature (<100.5 or >102.5°F), respiration rate (>20/min), heart rate (>102/min), and total leukocytes count (<6×103 or >16×103 per μL). The SIRS+ bitches showed hypoalbuminemia, hyperglobulinemia, elevated blood urea nitrogen and creatinine, decreased super oxide dismutase (SOD) activity with moderate increase in the lipid peroxidation. Further, the SIRS+ bitches had significantly higher serum PGFM concentration (6.83±0.7 vs. 4.12±0.4 ng/mL) than SIRS- and the level was influenced by cervical patency. It was concluded that elevated serum PGFM along with hyperglobunemia, blood urea nitrogen, creatinine would be useful in diagnosis and monitoring of pyometra led SIRS in bitch.

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Section: Resultssupporting

confidence: 51%

Effect of systemic inflammatory response syndrome (SIRS) on prostaglandin metabolite and oxidative stress in canine pyometra

Singh

Patra

Mishra

et al. 2020

Indian J of Anim Sci

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“…11,13 In addition, it has been demonstrated that proinflammatory cytokine genes, such as prostaglandin synthesis enzymes, are upregulated in the endometrium of female dogs suffering from CEH-P but not in normal diestrous dogs. [34][35][36] In vitro canine experiments have demonstrated the importance of prostaglandins in regulating uterine vascular tone. 37 Additionally, in dogs, acetylcholine-induced uterine artery vasodilatation probably occurs through the release of endotheliumderived relaxing factors, such as nitric oxide, endothelium-derived hyperpolarizing factor, and prostacyclin.…”

Section: Discussionmentioning

confidence: 99%

“…These findings may probably be due to chronic inflammation and/or persistent hyperplasia after the resolution of the pyometra 11,13 . In addition, it has been demonstrated that proinflammatory cytokine genes, such as prostaglandin synthesis enzymes, are upregulated in the endometrium of female dogs suffering from CEH‐P but not in normal diestrous dogs 34‐36 . In vitro canine experiments have demonstrated the importance of prostaglandins in regulating uterine vascular tone 37 .…”

Section: Discussionmentioning

confidence: 99%

Doppler ultrasonographic evaluation of medically treated female dogs with cystic endometrial hyperplasia–pyometra complex

Batista

Gobello

Rube

et al. 2022

Vet Radiology Ultrasound

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Monitoring the treatment outcome of canine cystic endometrial hyperplasia-pyometra complex (CEH-P) is essential to identify nonresponding patients who might require a rapid intervention to avoid life-threatening conditions. Uterine artery Doppler characterization may contribute to monitoring medically treated CEH-P, but published studies are currently lacking. Therefore, the aim of this longitudinal prospective observational study was to evaluate uterine artery resistance changes in the medical treatment of female dogs with CEH-P. Twelve diestrous female dogs suffering from CEH-P were treated with a combined protocol of cabergoline, cloprostenol, and antibiotics. The animals were clinically and ultrasonographically evaluated before the beginning of treatment (day 0) and on days 3, 7, 14, 21, and 28. The widest transverse diameter and luminal diameter of uterine horns were measured, as well as the peak systolic velocity (PSV) and end diastolic velocity (EDV) of uterine arteries. The resistance index (RI), pulsatility index (PI), and notch deep index (NDI) were calculated. On day 3, nine of 12 animals showed clinical improvement. In all these female dogs, the widest transverse sectional diameter (P < 0.01), luminal diameter (P < 0.01), PSV (P < 0.01), and EDV (P < 0.01) progressively decreased, while RI (P < 0.01), PI (P < 0.01), and NDI (P < 0.01) increased up to day 21. In nonresponding dogs (3/12), Doppler parameters remained unchanged. Although comparisons of PSV, EDV, and NDI were significantly different, these results should be cautiously interpreted due to the low statistical power. female dogs that responded to this treatment showed an increase in uterine artery resistance, along with clinical and ultrasonographic improvement.

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“…More recently, a high expression of inflammatory, cell proliferation and angiogenesis markers were verified in pyometra bitches, in comparison to CEHmucometra (Veiga et al, 2017). Additionally, Singh et al (2018) showed a higher expression of inflammatory cytokines in atrophic pyometra compared to hyperplastic pyometra. Thus, canine pyometra has its course marked by increased uterine blood flow and endometrial morphofunctional changes, along with important clinical signs that deserve special medical attention.…”

Section: Introductionmentioning

confidence: 92%

Prostaglandin and antigestagen in pyometra bitches: vascular and stereological effect

Filho

Brito

Faustino

et al. 2021

Reproduction and Fertility

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Effects of conservative treatment on uterine blood flow and morphometric findings are still unknown in bitches. Thus, this study aimed to compare uterine changes of pyometra bitches subjected to distinct modes of treatment. Pyometra bitches were assigned to: OHE (ovariohysterectomy immediately after diagnosis), Aglepristone (days 1, 2 and 8) and Associative (aglepristone treatment coupled with cloprostenol for 7 days) groups. After 9 days, bitches were ovariohysterectomized. Before surgery, uterine area was measured ultrasonographically, and uterine artery Doppler velocimetry analyzed blood flow velocity and indexes. Uterine horns were classified according to resistance index (RI) as more compromised and less compromised. Endometrial vasculature was quantitatively evaluated by color flow Doppler. Blood samples were collected to determine nitric oxide (NO) concentrations. Histological uterine structures were quantified by stereology and VEGF-A (vascular endothelial growth factor) and eNOS (endothelial nitric oxide synthase) immunohistochemically analyzed. Aglepristone and Associative groups had lower uterine area and vascularization, and higher blood flow velocity and indexes compared to OHE group. Less compromised horn of Associative group had higher blood flow velocity compared to OHE group. Aglepristone group presented lower inflammatory infiltrate and larger uterine stroma. Associative group had lower volume density and absolute surface of endometrial cysts and lower VEGF-A expression for glandular epithelium and stromal cells. Blood NO and e-NOS immunostaining were not different among groups. In conclusion, association between aglepristone and prostaglandin is more effective in decreasing uterine vascularization and modulating uterine blood flow. Moreover, associative therapy promotes marked morphological changes.

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Endometrial transcripts of proinflammatory cytokine and enzymes in prostaglandin synthesis are upregulated in the bitches with atrophic pyometra

Cited by 12 publications

References 29 publications

Effect of systemic inflammatory response syndrome (SIRS) on prostaglandin metabolite and oxidative stress in canine pyometra

Effect of systemic inflammatory response syndrome (SIRS) on prostaglandin metabolite and oxidative stress in canine pyometra

Doppler ultrasonographic evaluation of medically treated female dogs with cystic endometrial hyperplasia–pyometra complex

Prostaglandin and antigestagen in pyometra bitches: vascular and stereological effect

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