Endogenous Microbiota of the Genitourinary Tract

Martín, David H.; Ferris, Michael J.

doi:10.1016/b978-0-12-415847-4.00007-0

Cited by 4 publications

(3 citation statements)

References 99 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…are indicative of health, and their depletion with increased proportions of morphotypes resembling Gardnerella, Bacteroides spp., and curved Gram-variable rods is indicative of bacterial vaginosis. 24 …”

Section: Methodsmentioning

confidence: 99%

Bacterial Vaginosis and Associated Factors Among Pregnant Women Attending Antenatal Care in Harar City, Eastern Ethiopia

Ahmed¹,

Ayana²,

Abate³

2022

IDR

View full text Add to dashboard Cite

Background Bacterial vaginosis is one of the most common causes of abnormal vaginal discharge in women of reproductive age, especially pregnant women. It is characterized by the decrement of lactobacilli and increasing proliferation of diverse anaerobic bacteria. The prevalence of bacterial vaginosis and contributing factors vary between countries, within the same country, and among different populations. Objective The purpose of this study was to determine the prevalence of bacterial vaginosis and associated factors among pregnant women attending antenatal care from May 5–July 15, 2021 in health-care facilities found in Harar town, eastern Ethiopia. Methods and Materials An institutional-based cross-sectional study was conducted among 248 pregnant women attending antenatal care through systematic random sampling. Data were collected using a structured questionnaire and two vaginal swabs were collected using sterile swabs and transported to the microbiology laboratory. Samples were analyzed using Amsel’s criteria or using Nugent scoring criteria and culture. Data were entered into EpiData version 3.1 and transferred to SPSS version 25 for analysis. Binary logistic regression was used to identify variables associated with bacterial vaginosis. P-value <0.05 was considered statistically significant. Results Altogether 248 pregnant women were included in the study. Overall, 21.4% (95% CI: 16, 27) of study participants had bacterial vaginosis. History of sexually transmitted infection (AOR = 6.0, 95% CI: 1.94, 19.07; P = 0.002), history of spontaneous abortion (AOR = 5.8, 95% CI: 1.55, 22.02; P = 0.009), multiple sex partners (AOR = 8.6, 95% CI: 2.93, 25.79; P = 0.000) and having vaginal discharge (AOR = 5.5, 95% CI: 2.48, 12.41; P = 0.000) were significantly associated with bacterial vaginosis. Conclusion The prevalence of bacterial vaginosis is higher among symptomatic pregnant women and associated with a history of sexually transmitted infection, vaginal discharge, multiple sexual partners and spontaneous abortion.

show abstract

Section: Methodsmentioning

confidence: 99%

Bacterial Vaginosis and Associated Factors Among Pregnant Women Attending Antenatal Care in Harar City, Eastern Ethiopia

Ahmed¹,

Ayana²,

Abate³

2022

IDR

View full text Add to dashboard Cite

show abstract

“…Biomarkers such as PD-L1 expression, TILs, and immune gene signatures have been extensively investigated to forecast treatment response and patient outcomes. Furthermore, scholarly research has shed light on the role of the gut microbiome in modulating systemic immune responses, highlighting its potential as a therapeutic target in ovarian cancer (15)(16)(17)(18). To conclude, the immune microenvironment exerts a pivotal influence on ovarian cancer, encompassing tumor progression, immune evasion, and treatment response.…”

Section: The Role Of Immune Microenvironment In Ovarian Cancermentioning

confidence: 99%

Metabolic and senescence characteristics associated with the immune microenvironment in ovarian cancer

Xiong,

Fu,

Huang

et al. 2023

Front. Endocrinol.

View full text Add to dashboard Cite

Ovarian cancer is a highly malignant gynecological cancer influenced by the immune microenvironment, metabolic reprogramming, and cellular senescence. This review provides a comprehensive overview of these characteristics. Metabolic reprogramming affects immune cell function and tumor growth signals. Cellular senescence in immune and tumor cells impacts anti-tumor responses and therapy resistance. Targeting immune cell metabolism and inducing tumor cell senescence offer potential therapeutic strategies. However, challenges remain in identifying specific targets and biomarkers. Understanding the interplay of these characteristics can lead to innovative therapeutic approaches. Further research is needed to elucidate mechanisms, validate strategies, and improve patient outcomes in ovarian cancer.

show abstract

“…For vaginal microbiota studies, diverse 16S rRNA gene regions were applied for amplification and sequencing ( 1 , 6 , 8 , 17 – 20 ), which greatly affect the comparability between different studies as well as the species-level resolution of taxonomic classification ( 21 ). Despite the sequencing region of 16S rRNA gene, other factors can also influence the resolution of species-level classification.…”

Section: Introductionmentioning

confidence: 99%

Species-level resolution for the vaginal microbiota with short amplicons

Qing,

Shi,

Chen

et al. 2024

mSystems

View full text Add to dashboard Cite

Specific bacterial species have been found to play important roles in human vagina. Achieving high species-level resolution is vital for analyzing vaginal microbiota data. However, contradictory conclusions were yielded from different methodological studies. More comprehensive evaluation is needed for determining an optimal pipeline for vaginal microbiota. Based on the sequences of vaginal bacterial species downloaded from NCBI, we conducted simulated amplification with various primer sets targeting different 16S regions as well as taxonomic classification on the amplicons applying different combinations of algorithms (BLAST+, VSEARCH, and Sklearn) and reference databases (Greengenes2, SILVA, and RDP). Vaginal swabs were collected from participants with different vaginal microecology to construct 16S full-length sequenced mock communities. Both computational and experimental amplifications were performed on the mock samples. Classification accuracy of each pipeline was determined. Microbial profiles were compared between the full-length and partial 16S sequencing samples. The optimal pipeline was further validated in a multicenter cohort against the PCR results of common STI pathogens. Pipeline V1–V3_Sklearn_Combined had the highest accuracy for classifying the amplicons generated from both the NCBI downloaded data (84.20% ± 2.39%) and the full-length sequencing data (95.65% ± 3.04%). Vaginal samples amplified and sequenced targeting the V1–V3 region but merely employing the forward reads (223 bp) and classified using the optimal pipeline, resembled the mock communities the most. The pipeline demonstrated high F1-scores for detecting STI pathogens within the validation cohort. We have determined an optimal pipeline to achieve high species-level resolution for vaginal microbiota with short amplicons, which will facilitate future studies. IMPORTANCE For vaginal microbiota studies, diverse 16S rRNA gene regions were applied for amplification and sequencing, which affect the comparability between different studies as well as the species-level resolution of taxonomic classification. We conducted comprehensive evaluation on the methods which influence the accuracy for the taxonomic classification and established an optimal pipeline to achieve high species-level resolution for vaginal microbiota with short amplicons, which will facilitate future studies.

show abstract

Endogenous Microbiota of the Genitourinary Tract

Cited by 4 publications

References 99 publications

Bacterial Vaginosis and Associated Factors Among Pregnant Women Attending Antenatal Care in Harar City, Eastern Ethiopia

Bacterial Vaginosis and Associated Factors Among Pregnant Women Attending Antenatal Care in Harar City, Eastern Ethiopia

Metabolic and senescence characteristics associated with the immune microenvironment in ovarian cancer

Species-level resolution for the vaginal microbiota with short amplicons

Contact Info

Product

Resources

About