Endogenous<i>cis,trans</i>-Abscisic Acid and Pea Seed Development: Evidence for a Role in Seed Growth from Changes Induced by Temperature

Browning, G.

doi:10.1093/jxb/31.1.185

Cited by 31 publications

(18 citation statements)

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“…The estimate of ABA concentration in the cotyledons was lower in Table II than Table I, but this was not surprising as different cotyledon samples were used. Again, the estimates were similar to those in the published literature (2,5). The age of seeds strongly influences ABA levels (5) and this was not monitored carefully in the seeds used in the present study.…”

supporting

confidence: 87%

“…The age of seeds strongly influences ABA levels (5) and this was not monitored carefully in the seeds used in the present study. However, the seeds were nearing the end of a phase of maximum growth, so ABA levels can be expected to be close to the maximum level (2). In addition to the quantitative validation by two more definitive methods (GC-EC and GC-MS-SIM), the use of capillary GC-MS-SIM in the presence of [2H6]ABA gives a qualitative validation (i.e.…”

mentioning

confidence: 99%

See 1 more Smart Citation

The Development of an Indirect Enzyme Linked Immunoassay for Abscisic Acid

Ross¹,

Elder²,

McWha³

et al. 1987

Plant Physiol.

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supporting

confidence: 87%

mentioning

confidence: 99%

The Development of an Indirect Enzyme Linked Immunoassay for Abscisic Acid

Ross¹,

Elder²,

McWha³

et al. 1987

Plant Physiol.

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“…The relatively high levels (up to 12.2 #g g-' fresh weight) (15) and timing of ABA accumulation in soybean seeds (1,4,15) suggest a role in seed growth above simply preventing precocious germination. Browning (3) found that ABA accumulated more rapidly in a lare-seeded pea line and suggested a role for ABA in controlling cotyledon enlargement during linear seed fill. It has also been suggested that ABA may stimulate phloem unloading (8,18) by decreasing the proton motive force across the sieve tube plasmalemma.…”

Section: Abstracrmentioning

confidence: 99%

Abscisic Acid and Its Relationship to Seed Filling in Soybeans

Schussler¹,

Brenner²,

Brun³

1984

Plant Physiol.

134

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ABSTRACrThe effect of exogenous abscisic acid (ABA) on the rate of sucrose uptake by soybean (Glycine max L. Men.) embryos was evaluated in an in vitro system. In addition, the concentrations of endogenous ABA in seeds of three soybean Plant Introduction (PI) lines, differing in seed size, were commpared to their seed growth rates. ABA (10-molar) stimulated in vitro sucrose uptake in soybean (cv 'Cly') embryos removed from plants grown in a controled environment chamber, but not in embryos removed from field-grown plants of the Sink strength describes the capacity ofan organ to accumulate photoassimilates, and is the product of sink size and sink activity (25). Plant hormones are probably involved in determining both sink size and sink intensity. While cytokinins, gibberellins, and auxins generally promote growth processes, ABA has most often been considered an inhibitory substance (24). However, promotive effects of ABA in sink tissues have now been reported. ABA enhanced sugar content in roots of intact bean plants, possibly by stimulating sugar transport from the shoot to the root (11). In some cases, applications of ABA to ears of wheat (7) and barley (22) have stimulated 14C assimilate transfer from the flag leaf to the ear. However, King and Patrick (12) saw no stimulation of 14C transport to wheat ears after injection of ABA into the grains. Correlations between rate of growth and ABA accumulation in major sinks have been reported for wheat (13,14), grapes (5), pea (3), bean (10), and soybeans (4, 15). The relatively high levels (up to 12.2 #g g-' fresh weight) (15) and timing of ABA accumulation in soybean seeds (1,4,15) suggest a role in seed growth above simply preventing precocious germination. Browning (3) found that ABA accumulated more rapidly in a lare-seeded pea line and suggested a role for ABA in controlling cotyledon enlargement during linear seed fill. It has also been suggested that ABA may stimulate phloem unloading (8, 18) by decreasing the proton motive force across the sieve tube plasmalemma. The embryo-seed coat interface of the soybean seed (21) In addition, the endogenous ABA concentrations of seed coats, cotyledons, and embryonic axes of three field-grown soybean PI lines differing in seed size were compared to their seed growth rates during filling. MATERIALS AND METHODSGrowth Chamber Study. Plant Culture. Variety 'Clay' soybeans (Group 00 maturity) were grown in a controlled environment chamber in 1-L plastic pots in a 1.5:1.5:1 (v/v/v) mixture of vermiculite, peat, and sand. Pots were watered twice daily with automatic drip irrigation and given 250 ml of Peters 20-20-20 (200 ppm) twice weekly. A 14-h photoperiod with a 25/19C day/night temperature regime was provided. The photosynthetic photon flux density at the top of the canopy was 800 jE m-2 s-1.In Vitro Sucrose Uptake. During mid pod fill (seed dry weight 40% of final seed dry weight) pods were removed. Seed coats were surgically removed from a number of seeds and groups of three embryos were placed in 15 ml of sterile 10 ...

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“…ABA stimulated in vitro sucrose uptake by isolated soybean cotyledons (16) and sugar beet root discs (14). Decreased reproductive abscission in soybean in response to light was shown to be associated with increased partitioning of recently fixed carbon to the reproductive structures (8); in addition, carbon partitioning was greater in flowers destined to set pods than in flowers destined to abscise (4).The association of pod set with carbon partitioning (4, 9), and of carbon partitioning with ABA (3,6,16), suggests that there may be a positive relationship between pod set and ABA concentration of the flower. The objectives of this study were to determine: (a) if shading of individual reproductive structures has an effect on their endogenous ABA levels, (b) if shading of reproductive structures has an effect on ABA catabolism, and (c) if shading has an effect on partitioning of ABA and photoassimilates from leaves to reproductive structures.…”

mentioning

confidence: 99%

“…The association of pod set with carbon partitioning (4, 9), and of carbon partitioning with ABA (3,6,16), suggests that there may be a positive relationship between pod set and ABA concentration of the flower. The objectives of this study were to determine: (a) if shading of individual reproductive structures has an effect on their endogenous ABA levels, (b) if shading of reproductive structures has an effect on ABA catabolism, and (c) if shading has an effect on partitioning of ABA and photoassimilates from leaves to reproductive structures.…”

mentioning

confidence: 99%

Effect of Shading Individual Soybean Reproductive Structures on Their Abscisic Acid Content, Metabolism, and Partitioning

Yarrow

Brun²,

Brenner³

1988

Plant Physiol.

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Typically, a large proportion of soybean flowers produced, abscise before reaching maturity. Estimates as high as 57 to 82% abscission have been reported for determinate varieties (23, 27), and 32 to 65% for indeterminate types (23).Soybean floral abscission may be either stimulated or reduced by environmental factors. Nutrient deficiency (2), high temperatures, (24), long photoperiods (2, 24) and reduced light intensity (9, 15, 24) stimulate abscission, while increasing the available N (2) and CO2 during flowering (7) (13) in studies of partitioning to rose shoot buds.ABA has been reported to be related to abscission of various plant structures (1). However, recent evidence suggests a role of ABA in the partitioning of photoassimilates to developing fruit and seeds. A positive relationship between exogenously applied ABA and sink activity has been shown in both wheat (6) and barley (22). In peas (3) and soybeans (16), a positive relationship was observed between seed growth rate and ABA concentration. ABA stimulated in vitro sucrose uptake by isolated soybean cotyledons (16) and sugar beet root discs (14). Decreased reproductive abscission in soybean in response to light was shown to be associated with increased partitioning of recently fixed carbon to the reproductive structures (8); in addition, carbon partitioning was greater in flowers destined to set pods than in flowers destined to abscise (4).The association of pod set with carbon partitioning (4, 9), and of carbon partitioning with ABA (3,6,16), suggests that there may be a positive relationship between pod set and ABA concentration of the flower. The objectives of this study were to determine: (a) if shading of individual reproductive structures has an effect on their endogenous ABA levels, (b) if shading of reproductive structures has an effect on ABA catabolism, and (c) if shading has an effect on partitioning of ABA and photoassimilates from leaves to reproductive structures. MATERIALS AND METHODSPlant Material. The semideterminate soybean genotype IX93-100 was grown in four completely randomized blocks in the field at St. Paul, MN in the summer of 1983. This genotype was selected for its relatively long racemes, allowing for easy determination of anthesis date, growth, development, and abscission of individual flowers. Seeds of IX93-100 were donated by C. D. Dybing, USDA-ARS, Brookings, SD.IX93-100 was selected from crosses made by D. E. Green, Iowa State University, of A71-5558-1 and L61-344, a semideter-

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Endogenouscis,trans-Abscisic Acid and Pea Seed Development: Evidence for a Role in Seed Growth from Changes Induced by Temperature

Cited by 31 publications

References 0 publications

The Development of an Indirect Enzyme Linked Immunoassay for Abscisic Acid

The Development of an Indirect Enzyme Linked Immunoassay for Abscisic Acid

Abscisic Acid and Its Relationship to Seed Filling in Soybeans

Effect of Shading Individual Soybean Reproductive Structures on Their Abscisic Acid Content, Metabolism, and Partitioning

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