Endogenous Ca<sup>2+</sup>Buffer Concentration and Ca<sup>2+</sup>Microdomains in Hippocampal Neurons

Müller, Andreas; Kukley, Maria; Stausberg, Pia; Beck, Hanno; Müller, Wolfgang; Dietrich, Dirk

doi:10.1523/jneurosci.3799-04.2005

Cited by 111 publications

(124 citation statements)

References 39 publications

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“…Neuronal calcium levels are strictly regulated by calcium-binding proteins. These include the calbindin--D28k (CB), a buffering and sensory protein (13,21).…”

Section: Praca Oryginalnamentioning

confidence: 99%

“…These cells are the excitatory glutamatergic neurons constituting 90% of all neurons of this area. Moreover, CB is localised in a small number of interneurons in SM and SG, in basket cells under or in SG and in H (21,24,25). The protein stimulates granular cells-mossy fibres system increasing a release of neurotransmitters in the nerve endings Influence of oral administration of HMB to pregnant dams on calbindin expression in the dentate gyrus of the hippocampus during postnatal development in spiny mice offspring of hippocampal CA3 region (7,31).…”

Section: Praca Oryginalnamentioning

confidence: 99%

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Influence of oral administration of HMB to pregnant dams on calbindin expression in the dentate gyrus of the hippocampus during postnatal development in spiny mice offspring

Rycerz

Krawczyk

Jaworska-Adamu

et al. 2017

Medycyna Weterynaryjna

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Influence of oral administration of HMB to pregnant dams on calbindin expression in the dentate gyrus of the hippocampus during postnatal development in spiny mice offspring Summary The aim of the study was to investigate the morphology, density and immunostaining intensity of calbindin (CB)-positive neurons of dentate gyrus (DG) in new-born (P0) and 21-day-old (P21) male Acomys cahirinus mice from dams receiving β-hydroxy-β-methylbutyrate (HMB) during pregnancy. Different substances administrated to pregnant dams may affect the calcium ion homeostasis which is crucial for the proper brain development of their offspring. DG with hilus (H) plays an important role in memory and learning processes. Calcium levels in DG are regulated by buffering proteins like calbindin D28k (CB). Experimental dams were orally treated with HMB at a dose of 0.2 g/kg b.w. Half of new-born animals were euthanised after birth and the rest after the 21 st day of life. The brains were dissected and embedded in paraffin blocks using a routine histological technique. In order to demonstrate CB protein expression an immunohistochemical peroxidase-antiperoxidase reaction was conducted. The results of the study did not reveal important morphological alterations. There were no statistically significant changes in the density of the studied cells either in P0 and P21 animals. However, the authors have demonstrated a statistically significant increase of the average CB-immunostaining intensity in nuclei and cytoplasm in both age groups. It may be a result of a compensation effect to alterations that occurred under the influence of HMB. On the basis of the conducted research, it may be assumed that HMB activity in DG may provide long-term consequences.

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“…Neuronal calcium levels are strictly regulated by calcium-binding proteins. These include the calbindin--D28k (CB), a buffering and sensory protein (13,21).…”

Section: Praca Oryginalnamentioning

confidence: 99%

Section: Praca Oryginalnamentioning

confidence: 99%

Influence of oral administration of HMB to pregnant dams on calbindin expression in the dentate gyrus of the hippocampus during postnatal development in spiny mice offspring

Rycerz

Krawczyk

Jaworska-Adamu

et al. 2017

Medycyna Weterynaryjna

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“…In other cells, calretinin concentration is much lower: approximately 20 mM and 35 mM in rat inner and outer hair cells, respectively (Hackney et al 2005), and 30 -40 mM in mouse cerebellar granule cells (Gall et al 2003). The concentration of CB-D28k is 150 -360 mM in Purkinje cells (for a review, see Schwaller et al 2002), and 40 -50 mM in mature hippocampal dentate gyrus granule cells, CA3 interneurons, and in CA1 pyramidal cells (Muller et al 2005). In cells expressing various mobile and immobile Ca 2þ buffers of unknown identities and Ca 2þ -binding properties, the concept of the Ca 2þ -binding ratio of endogenous buffers has proven to be useful.…”

Section: Important Parameters To Characterize Ca 2þ Buffers Intracellmentioning

confidence: 99%

Cytosolic Ca2+ Buffers

Schwaller¹

2010

Cold Spring Harbor Perspectives in Biology

345

305

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“…In conclusion, the available data are consistent with the view that transmitter release at hippocampal mossy fiber synapses is triggered by a large number of presynaptic Ca 2+ channels, which might result in a precisely timed presynaptic Ca 2+ signal even in the presence of stochastic single channel gating. However, the distance between Ca 2+ source and Ca 2+ sensor may allow modulation of the amplitude of the presynaptic Ca 2+ transient by endogenous Ca 2+ buffers, such as calbindin-D 28k [62].…”

Section: Abstract Presynaptic Recording Mossy Fiber Boutons Mossymentioning

confidence: 99%

Timing and efficacy of transmitter release at mossy fiber synapses in the hippocampal network

Bischofberger

Engel

Frotscher

et al. 2006

Pflugers Arch - Eur J Physiol

114

110

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It is widely accepted that the hippocampus plays a major role in learning and memory. The mossy fiber synapse between granule cells in the dentate gyrus and pyramidal neurons in the CA3 region is a key component of the hippocampal trisynaptic circuit. Recent work, partially based on direct presynaptic patch-clamp recordings from hippocampal mossy fiber boutons, sheds light on the mechanisms of synaptic transmission and plasticity at mossy fiber synapses. A high Na + channel density in mossy fiber boutons leads to a large amplitude of the presynaptic action potential. Together with the fast gating of presynaptic Ca 2+ channels, this generates a large and brief presynaptic Ca 2+ influx, which can trigger transmitter release with high efficiency and temporal precision. The large number of release sites, the large size of the releasable pool of vesicles, and the huge extent of presynaptic plasticity confer unique strength to this synapse, suggesting a large impact onto the CA3 pyramidal cell network under specific behavioral conditions. The characteristic properties of the hippocampal mossy fiber synapse may be important for pattern separation and information storage in the dentate gyrus-CA3 cell network.Keywords Presynaptic recording . Mossy fiber boutons . Mossy fiber synapses . Hippocampus . Autoassociative networks . Episodic memory . Synaptic efficacyThe mossy fiber synapse: a key connection in the hippocampal networkThe hippocampal formation consists of three types of principal neurons: granule cells in the dentate gyrus, CA3 pyramidal cells, and CA1 pyramidal neurons. These cells are interconnected by glutamatergic synapses, forming the classical trisynaptic circuit (Fig. 1a,b). Dentate gyrus granule cells receive excitatory glutamatergic input from layer 2 pyramidal cells of the entorhinal cortex and project to CA3 pyramidal cells. CA3 pyramidal cells project to CA1 cells, which in turn project to the subiculum and back to the entorhinal cortex [4]. In addition to this trisynaptic circuit, there is also a direct input from the entorhinal cortex to both CA3 and CA1 pyramidal cells. Furthermore, CA3 pyramidal neurons are extensively connected to each other via recurrent collateral synapses.The nonmyelinated axons of the granule cells, the socalled mossy fibers, show several structural properties that distinguish them from the other synaptic pathways [39]. They project to the CA3 region, mainly traveling within a narrow band termed "stratum lucidum." Several (∼15) large "giant" boutons (∼3-5-μm diameter) emerge from a single mossy fiber axon, either arranged in an en passant manner or attached to the main axon via a short perpendicular axonal branch [1,8,29,31] (Fig. 1c,d). A large mossy fiber bouton typically contacts only a single CA3 pyramidal neuron [21], and a mossy fiber axon contacts a given CA3 pyramidal neuron only once, as boutons are ∼150 μm apart. As the rat hippocampus contains ∼1 million granule cells

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Endogenous Ca²⁺Buffer Concentration and Ca²⁺Microdomains in Hippocampal Neurons

Cited by 111 publications

References 39 publications

Influence of oral administration of HMB to pregnant dams on calbindin expression in the dentate gyrus of the hippocampus during postnatal development in spiny mice offspring

Influence of oral administration of HMB to pregnant dams on calbindin expression in the dentate gyrus of the hippocampus during postnatal development in spiny mice offspring

Cytosolic Ca2+ Buffers

Timing and efficacy of transmitter release at mossy fiber synapses in the hippocampal network

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Endogenous Ca2+Buffer Concentration and Ca2+Microdomains in Hippocampal Neurons

Cited by 111 publications

References 39 publications

Influence of oral administration of HMB to pregnant dams on calbindin expression in the dentate gyrus of the hippocampus during postnatal development in spiny mice offspring

Influence of oral administration of HMB to pregnant dams on calbindin expression in the dentate gyrus of the hippocampus during postnatal development in spiny mice offspring

Cytosolic Ca2+ Buffers

Timing and efficacy of transmitter release at mossy fiber synapses in the hippocampal network

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Endogenous Ca²⁺Buffer Concentration and Ca²⁺Microdomains in Hippocampal Neurons