Endogenous auxin levels in terminal stem cuttings of Chrysanthemum morifolium during adventitious rooting

Abstract

Weigel, U., Horn, W. and Hock, B. 1984. Endogenous auxin levels in stem cuttings of Chrysatuhemum tnorifoUum during adventitious rooting. -Physiol. Plant 61 • 422-428.Free and ester-bound IAA were determined in Chtysattthemum tnorifoliutn Ratnat cv. 'Yellow Galaxy' by means of a solid phase enzyme immunoassay. In shoots, free auxin decteases basipetally whereas ester IAA teaches a maximum in the middle part. After making the cuttings, a strong increase in both free and ester iAA (or total IAA, respectively) is found up to the time when the first adventitious roots become visible. Only prolonged irradiance of stock plants at high light intensities (40 W tn--) delays this increase in the cuttings, concomitantly with a lower number of roots compared to the controls (4.5 W m-), although root growth as determined by measuring root length or fresh weight is not affected. A distinct relation is found between IAA content of stock plants at the time when cuttings are taken and the number of adventitious roots formed by the cuttings 20 days later.Additional key words -^nzyrac immunoassay, light.U. Weigel and B. Hock (reprint requests), Lehrstuhl fiir Botanik, Fakidtdt Landwirtschaft und

Effect of Indole 3-Butyric Acid and Media Type on Adventitious Root Formation in Sheanut Tree (<i>Vitellaria paradoxa</i> C. F. Gaertn.) Stem Cuttings

“…This was also supported by [12] who found that carbohydrate aids in auxin transport as well as growth of the roots and increased number of roots per cutting. [13] also speculated that mobilization of carbohydrate in the leaves and upper stem to the rooting zone is influenced by hormone application.…”

Euphorbia escula L. Root and Root Bud Indole-3-Acetic Acid Levels at Three Phenologic Stages

“…Chrysanthemum morifolium shoot tip LAA levels ranged from 15.6 to 29.0 ng/g FW, which is also considerably lower than E. esula root bud-free LAA levels. On the other hand, E. esula primary root-free IAA levels were very similar to levels of free IAA reported for primary root tissue of Zea mays (21), vegetative tissue of Z. mays, Pisum sativum (1,2), and Avena sativa (2), and also similar to free IAA levels in stem cuttings of C. morifolium (25).…”

Enraizamento de miniestacas de clones híbridos de Eucalyptus globulus

“…Kawaoka et al (2010) observaram que clones de Eucalyptus globulus que apresentaram maiores taxas de enraizamento (90 %) em sistema de micropropagação tinham três vezes mais ácido indol-3-acético (AIA) endógeno do que clones com baixo enraizamento (10 %). Aumento no conteúdo de AIA também foi observado em estacas de Chrysanthemum morifolium (WEIGEL et al, 1984) e de videira (GASPAR et al, 1992) logo após a coleta das estacas nas plantas matrizes. No entanto, mais estudos deveriam ser implementados com Eucalyptus, relacionando o enraizamento de estacas com a expressão de auxinas endógenas e também com o balanço interno de fitorreguladores, como a relação auxina/citocinina.…”