Endogenous auxin level is a critical determinant for in vitro adventitious shoot regeneration in potato (Solanum tuberosum L.)

Pal, Awadhesh Kumar; Acharya, Karan; Ahuja, Paramvir Singh

doi:10.1007/s13562-011-0092-z

Cited by 25 publications

(16 citation statements)

References 20 publications

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“…Shoot organogenesis protocol, a pre-requisite for genetic manipulation of plants has been well documented in potato but is complicated by use of two step procedure (Yee et al 2001, Yasmin et al 2003, Elaleem et al 2009, Molla et al 2011, Rezende et al 2013, Ghosh et al 2014, Abdelaleem 2015, Campos et al 2016. Moreover, genotype specific response of potato cultivars to plant growth regulators (PGRs) has also been reported by many researchers (Khokan et al 2009, Pal et al 2011, Wheeler et al 1985. In this context, previously we have developed one step, genotype independent shoot organogenesis protocol for potato (Kaur et al 2017).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Gelling agent, medium pH and silver nitrate on adventitious shoot regeneration inSolanum tuberosum

Kaur

Kumar

2020

Preprint

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Vegetative propagation of potato makes the crop vulnerable to many seed borne diseases. The importance of the crop in attainment of food security makes it an important candidate for in vitro propagation and genetic manipulations. To undertake crop improvement programmes, development of an efficient regeneration protocol is a pre-requisite. Therefore, the present report was focussed to study various factors affecting shoot organogenesis in potato cultivar 'Kufri Chipsona 1'. The incorporation of silver nitrate (10 µM) to the regeneration medium (MS medium supplemented with BA and GA3) was found to induce shoot organogenesis in 32.11% of leaf and 59.99% of internodal explants. An increase in mean number of shoots regenerated per leaf (5.31) and internodal (8.67) explant was also observed upon addition of silver nitrate to the medium. Similarly, solidification of medium with clarigel and its adjustment to pH 5.8 was found optimum for increasing shoot organogenesis frequency in potato. Among the two types of explants tested, a better response was observed from internodes in comparison with leaf explants. The regenerated shoots were tested for clonal fidelity using PCR based molecular markers [Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeats (ISSR)] and were found true to type.

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Section: Discussionmentioning

confidence: 99%

The Effect of Gelling agent, medium pH and silver nitrate on adventitious shoot regeneration inSolanum tuberosum

Kaur

Kumar

2020

Preprint

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“…Many studies have shown that callus induction in potato could be influenced by a variety of factors in- cluding genotype, explant type, and plant growth regulator (type and concentration) (Hagen et al 1990;JayaSree et al 2001;Yee et al 2001;Tican et al 2008;Dokhaniyeh et al 2011;Pal et al 2012). The present finding that both leaf and internodal explants of potato (cv.…”

Section: Discussionmentioning

confidence: 54%

Differential cadmium resistance of two morphologically distinct types of potato (Solanum tuberosum) callus

et al. 2015

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Callus culture has been used to study the cellular basis of sensitivity of plants to toxic trace elements including cadmium. Callus friability may be related to plant response to exposure to trace elements as callus friability is related to the plant cell wall which plays a role in resistance of plant cells to trace element toxicity. The aim of this study was to investigate the relationship between two types of potato callus with different friability and their sensitivity to cadmium. A high frequency (about 80%) of leaf or internodal explants of potato (Solanum tuberosum L., cv, Iwa) formed a friable, pale green callus (type-A callus) on half-strength basal Murashige and Skoog medium supplemented with 12.42 µM picloram. On medium supplemented with 4.43 µM 6-benzyladenine (BA) and 5.37 µM 1-naphthalene acetic acid (NAA), a compact, non-friable callus (type-B callus) was induced in about 80% of the explants. Type-B callus was greener than type-A callus. Callus formation in both the leaf and internodal explants were completely inhibited on the medium used for induction of type-A or type-B callus when the respective medium was supplemented with 54 µM cadmium chloride (Cd). The type-B callus was found to be more resistant (less necrosis and higher relative growth rates) to 27, 54, and 109 µM of Cd than the type-A callus. The type-B callus also exhibited a higher level of peroxidase activity (a marker antioxidant enzyme counteracting oxidative stress) than the type-A callus when cultured on these Cd concentrations. This is the first study showing the importance of callus friability in plant cell response to Cd treatment.

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“…Whether it is the intact plant grown in the field or one of these experimental systems, the tuberization process is accompanied by morphological and anatomical changes in the differentiated cells at the subapical region of the stolon or lateral shoot 5 . Potato callus is an unorganised tissue formed following de-differentiation of cells derived from, for example, excised potato leaf 6 , internodal 7 or tuber segments 8 under plant tissue culture conditions. Plant regeneration via de novo organogenesis [7][8][9][10] or somatic embryogenesis 6 in potato callus has been shown.…”

Section: Introductionmentioning

confidence: 99%

“…Potato callus is an unorganised tissue formed following de-differentiation of cells derived from, for example, excised potato leaf 6 , internodal 7 or tuber segments 8 under plant tissue culture conditions. Plant regeneration via de novo organogenesis [7][8][9][10] or somatic embryogenesis 6 in potato callus has been shown. In our unpublished observations on potato callus cultures initiated from leaf explants, unexpectedly microtuber development was found.…”

Section: Introductionmentioning

confidence: 99%

Microtuber formation in potato callus

Ashrafzadeh¹,

Leung²

2015

ScienceAsia

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Potato callus has been shown to be capable of de novo organized development including shoot and root formation. A surprising finding in a study of potato (Solanum tuberosum cv. Iwa) callus culture initiated from leaf explants on a medium supplemented with auxin and cytokinin is reported here. Five weeks after callus formation, a low frequency (about 10%) of the callus cultures had formed microtubers. The microtubers initially appeared to be light green and some were elongated. One week later the microtubers had lost much of the green appearance and were full of hairs on the side and with many scattered dark brown spots on the top view. Histological analysis showed that a microtuber was connected with the callus and not with the leaf explant as the callus typically lacked the internal organization of a leaf. This is the first report of the occurrence of microtubers in potato callus cultures.

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Endogenous auxin level is a critical determinant for in vitro adventitious shoot regeneration in potato (Solanum tuberosum L.)

Cited by 25 publications

References 20 publications

The Effect of Gelling agent, medium pH and silver nitrate on adventitious shoot regeneration inSolanum tuberosum

The Effect of Gelling agent, medium pH and silver nitrate on adventitious shoot regeneration inSolanum tuberosum

Differential cadmium resistance of two morphologically distinct types of potato (Solanum tuberosum) callus

Microtuber formation in potato callus

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