The Effect of Gelling agent, medium pH and silver nitrate on adventitious shoot regeneration inSolanum tuberosum

Abstract

Vegetative propagation of potato makes the crop vulnerable to many seed borne diseases. The importance of the crop in attainment of food security makes it an important candidate for in vitro propagation and genetic manipulations. To undertake crop improvement programmes, development of an efficient regeneration protocol is a pre-requisite. Therefore, the present report was focussed to study various factors affecting shoot organogenesis in potato cultivar 'Kufri Chipsona 1'. The incorporation of silver nitrate (10 µM) to the regeneration medium (MS medium supplemented with BA and GA3) was found to induce shoot organogenesis in 32.11% of leaf and 59.99% of internodal explants. An increase in mean number of shoots regenerated per leaf (5.31) and internodal (8.67) explant was also observed upon addition of silver nitrate to the medium. Similarly, solidification of medium with clarigel and its adjustment to pH 5.8 was found optimum for increasing shoot organogenesis frequency in potato. Among the two types of explants tested, a better response was observed from internodes in comparison with leaf explants. The regenerated shoots were tested for clonal fidelity using PCR based molecular markers [Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeats (ISSR)] and were found true to type.

Boosting Polyamines to Enhance Shoot Regeneration in Potato (Solanum tuberosum L.) Using AgNO3

“…The response of the related parameters to AgNO3 concentration assumed a more or less concentration-dependent bell-shaped curve peaked at 4 mg L −1 . With a genotype-dependent peak, the same trend was recorded for shoot regeneration in response to the increasing concentrations of AgNO3 in the potato cultivar 'Kufri Chipsona 1′ [25,26,39], tomato [40], primrose [41], oat [42], and wheat [43]. It was also observed dealing with shoot growth and chlorophyll content in potato [26] and robusta coffee [44].…”

The Past, Present and Future of Cannabis sativa Tissue Culture

“…Generally, a culture medium can be categorized as a semi-solid or liquid medium. Although the concentration of gelling agents plays a conspicuous role in regeneration efficiency [ 132 ], there are no reports for Cannabis comparing the different types and concentrations of gelling agents. Carbohydrates are essential for many species in culture and there are many different sources (sucrose, glucose, fructose, maltose, glycerol, etc.).…”

The Effect of Zinc, Copper, and Silver Ions on Oat (Avena sativa L.) Androgenesis

“…Easy water solubility and chemical stability make it very useful in the regulation of morphogenesis in vivo and in vitro [ 39 ]. AgNO 3 has been shown to be effective in improving somatic embryogenesis and plant regeneration in a number of dicotyledonous species—for example, Punica granatum and Solanum tuberosum [ 40 , 41 ]. It has also been used successfully in cereal crops, such as rice [ 42 ], maize [ 43 ], pearl millet [ 44 ], and barley [ 45 ].…”