Endogenous auxin accumulation/localization during zygotic and somatic embryogenesis of Capsicum chinense Jacq

Pérez-Pastrana, Jacobo; Testillano, P. S.; Bárány, Ivett; Canto‐Flick, Adriana; Álvarez-López, Dulce; Pijeira‐Fernández, Gema; Avilés-Viñas, Susana A.; Peña-Yam, Laura P.; Muñoz-Ramírez, Liliana S.; Islas‐Flores, Ignacio; Santana‐Buzzy, Nancy

doi:10.1016/j.jplph.2020.153333

Cited by 9 publications

(7 citation statements)

References 55 publications

(65 reference statements)

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“…In Arabidopsis zygotic embryogenesis, an increase in auxin levels was observed in ovule tissues after fertilization [ 72 ], as well as in zygotic embryo cells from the very beginning of their formation [ 18 , 73 ]. Several studies have demonstrated a correlation between IAA levels and localization during somatic embryogenesis and in vivo zygotic embryogenesis in several species [ 43 , 74 , 75 ]. Regarding the distribution pattern of auxin in the zygotic embryo, several in vitro studies have detected a peak signal in the apical meristem of the stem and root in maize and barley [ 56 , 76 , 77 ], as well as in the apical zone of cotyledons in several dicotyledonous plants [ 21 , 25 , 43 ], coinciding with regions of maximum auxin biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

“…These results align with the observed polarization of auxin in cotyledonary embryos using immunofluorescence techniques, where a differential auxin accumulation pattern is observed, particularly with higher levels in the apical region of cotyledons and the basal zone of the embryo, areas with higher proliferation activity in the embryo. In several species, it has been also observed that during the early stages of zygotic embryogenesis, there is an auxin flow from the suspensor to the rest of the embryo [ 18 , 73 , 75 ]. Our results indicate that some embryos derived from microspores in the globular or heart stage can develop while retaining the suspensor structure.…”

Section: Discussionmentioning

confidence: 99%

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Opposite Auxin Dynamics Determine the Gametophytic and Embryogenic Fates of the Microspore

Pérez-Pérez

Solís

Albacete

et al. 2023

IJMS

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The microspore can follow two different developmental pathways. In vivo microspores follow the gametophytic program to produce pollen grains. In vitro, isolated microspores can be reprogrammed by stress treatments and follow the embryogenic program, producing doubled-haploid embryos. In the present study, we analyzed the dynamics and role of endogenous auxin in microspore development during these two different scenarios, in Brassica napus. We analyzed auxin concentration, cellular accumulation, the expression of the TAA1 auxin biosynthesis gene, and the PIN1-like efflux carrier gene, as well as the effects of inhibiting auxin biosynthesis by kynurenine on microspore embryogenesis. During the gametophytic pathway, auxin levels and TAA1 and PIN1-like expression were high at early stages, in tetrads and tapetum, while they progressively decreased during gametogenesis in both pollen and tapetum cells. In contrast, in microspore embryogenesis, TAA1 and PIN1-like genes were upregulated, and auxin concentration increased from the first embryogenic divisions. Kynurenine treatment decreased both embryogenesis induction and embryo production, indicating that auxin biosynthesis is required for microspore embryogenesis initiation and progression. The findings indicate that auxin exhibits two opposite profiles during these two microspore developmental pathways, which determine the different cell fates of the microspore.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Opposite Auxin Dynamics Determine the Gametophytic and Embryogenic Fates of the Microspore

Pérez-Pérez

Solís

Albacete

et al. 2023

IJMS

Self Cite

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“…A large number of TFs have been reported to regulate cell fate differentiation events, which determine the first zygotic division during embryogenesis. For example, the MYB TF family is expressed in early seed development ( Avilés-Viñas et al, 2021 ). Moreover, MYB interacts with bHLH to regulate seed maturation.…”

Section: Discussionmentioning

confidence: 99%

“…WOX genes (WOX2, WOX8, WOX1, and WOX3) play an important role during the ZE development. WOX2 is expressed in the apical daughter cell, and WOX8 is specifically expressed in the basal daughter cells of the zygote ( Sakakibara et al, 2014 ; Armenta-Medina et al, 2021 ; Avilés-Viñas et al, 2021 ). The YDA-MAP signaling cascade phosphorylates and activates WRKY2 to promote WOX8 transcription during zygote development ( Ueda et al, 2011 ; Horstman et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Time-Series Analyses of Gene Expression Profile During Zygotic Embryo Development in Picea mongolica

et al. 2021

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Zygotic embryogenesis is a critical process during seed development in gymnosperms. However, knowledge on the genome-wide transcriptional activation that guides this process in conifers is limited, especially in Picea mongolica. This tree species is endemic to semiarid habitats of Inner Mongolia in China. To extend what is known about the molecular events underpinning its zygotic embryogenesis, comparative transcriptomic analyses of gene expression in zygotic embryos were performed by RNA sequencing in P. mongolica. Our results showed that most changes in transcript levels occurred in the early embryonic pattering determination and formation of mature embryos. Transcripts related to embryogenic competence, cell division pattern, hormones, and stress response genes were identified during embryogenesis. Auxin is essential for early embryo patterning and pre-cotyledon embryonic formation. However, ABA is a major regulator of embryo maturation. Moreover, we found that methylation-related gene expression is associated with activation of early-stage embryos, late embryogenesis abundant proteins, and storage/energy-related genes with late and mature embryos. Furthermore, network analysis revealed stage-specific and multistage gene expression clusters during embryogenesis. WOX, MYB, AP2, and HLH transcription factors seem more relevant to embryogenesis in different stages. Our results provide large-scale and comprehensive transcriptome data for embryo development in P. mongolica. These data will lay a foundation for the protection and utilization of P. mongolica resources.

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“…This aligns with findings that cellular IAA content rises during somatic embryogenesis induction. 14 In some plant species, like Dendrobium, thidiazuron (TDZ) in the induction medium directly promotes somatic embryo production from leaf tips. 15 Shen et al 16 confirmed this, observing directly induced somatic embryos in Phalaenopsis with benzyl adenine (BA) and TDZ.…”

Section: Introductionmentioning

confidence: 99%

Enhancing in vitro regeneration via somatic embryogenesis and Fusarium wilt resistance of Egyptian cucumber (Cucumis sativus L.) cultivars

Hamza,

Diab,

Khatab

et al. 2024

Journal of Genetic Engineering and Biotechnology

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Endogenous auxin accumulation/localization during zygotic and somatic embryogenesis of Capsicum chinense Jacq

Cited by 9 publications

References 55 publications

Opposite Auxin Dynamics Determine the Gametophytic and Embryogenic Fates of the Microspore

Opposite Auxin Dynamics Determine the Gametophytic and Embryogenic Fates of the Microspore

Transcriptomic Time-Series Analyses of Gene Expression Profile During Zygotic Embryo Development in Picea mongolica

Enhancing in vitro regeneration via somatic embryogenesis and Fusarium wilt resistance of Egyptian cucumber (Cucumis sativus L.) cultivars

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