Endocytic regulation of alkali metal transport proteins in mammals, yeast and plants

Mulet, José; Llopis-Torregrosa, Vicent; Primo, Cecilia; Marques, María Carmen; Yenush, Lynne

doi:10.1007/s00294-013-0401-2

Cited by 16 publications

(17 citation statements)

References 260 publications

(247 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ubiquitylation plays an important role in this dynamic process in yeast, as well as in mammals and plants (1). In the case of yeast and mammals, HECT family E3 ubiquitin ligases, like Rsp5 (yeast) and Nedd4.2 (mammalian), have been shown to specifically target plasma membrane transport proteins.…”

Section: Discussionmentioning

confidence: 99%

“…The general mechanisms controlling this regulatory process are highly conserved among eukaryotic organisms and involve both transcriptional regulation and modulation of the balance of secretion, recycling, and degradation of individual transporter proteins in response to changes in the extracellular environment (reviewed in Ref. 1). Regulated endocytosis, one important step in this process, has been well studied in both mammals and yeast, unveiling many mechanistic similarities that establish yeast as a relevant model system.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Regulation of the Yeast Hxt6 Hexose Transporter by the Rod1 α-Arrestin, the Snf1 Protein Kinase, and the Bmh2 14-3-3 Protein

Llopis-Torregrosa

Ferri-Blázquez

Adam‐Artigues

et al. 2016

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Cell viability requires adaptation to changing environmental conditions. Ubiquitin-mediated endocytosis plays a crucial role in this process, because it provides a mechanism to remove transport proteins from the membrane. Arrestin-related trafficking proteins are important regulators of the endocytic pathway in yeast, facilitating selective ubiquitylation of target proteins by the E3 ubiquitin ligase, Rsp5. Specifically, Rod1 (Art4) has been reported to regulate the endocytosis of both the Hxt1, Hxt3, and Hxt6 glucose transporters and the Jen1 lactate transporter. Also, the AMP kinase homologue, Snf1, and 14-3-3 proteins have been shown to regulate Jen1 via Rod1. Here, we further characterized the role of Rod1, Snf1, and 14-3-3 in the signal transduction route involved in the endocytic regulation of the Hxt6 high affinity glucose transporter by showing that Snf1 interacts specifically with Rod1 and Rog3 (Art7), that the interaction between the Bmh2 and several arrestin-related trafficking proteins may be modulated by carbon source, and that both the 14-3-3 protein Bmh2 and the Snf1 regulatory domain interact with the arrestin-like domain containing the N-terminal half of Rod1 (amino acids 1-395). Finally, using both co-immunoprecipitation and bimolecular fluorescence complementation, we demonstrated the interaction of Rod1 with Hxt6 and showed that the localization of the Rod1-Hxt6 complex at the plasma membrane is affected by carbon source and is reduced upon overexpression of SNF1 and BMH2.An important component of the maintenance of cellular homeostasis and stress responses is the regulation of the composition of plasma membrane proteins responsible for the uptake and extrusion of nutrients and other nonpermeable small molecules. The general mechanisms controlling this regulatory process are highly conserved among eukaryotic organisms and involve both transcriptional regulation and modulation of the balance of secretion, recycling, and degradation of individual transporter proteins in response to changes in the extracellular environment (reviewed in Ref. 1). Regulated endocytosis, one important step in this process, has been well studied in both mammals and yeast, unveiling many mechanistic similarities that establish yeast as a relevant model system.In Saccharomyces cerevisiae, plasma membrane transporters that need to be down-regulated are ubiquitylated by the Rsp5 E3 ubiquitin ligase, endocytosed, sorted into multivesicular bodies in a process requiring the ESCRT machinery, and finally are delivered to the vacuole for degradation (2-5). Transporter ubiquitylation by the HECT family Rsp5 E3 ubiquitin ligase is mediated by one or more of several adaptor proteins that confer specificity and ensure the correct regulation of targeted proteins in response to changes in the extracellular environment (reviewed in Refs. 6). At least 18 Rsp5 adaptor proteins have been described, each having specificity for a subset of transport proteins and acting in response to specific stimuli.The molecular mechanisms governing th...

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Regulation of the Yeast Hxt6 Hexose Transporter by the Rod1 α-Arrestin, the Snf1 Protein Kinase, and the Bmh2 14-3-3 Protein

Llopis-Torregrosa

Ferri-Blázquez

Adam‐Artigues

et al. 2016

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…There are similarities in the way BOR1 and IRT1 exploit the endocytic pathway for regulating their respective minerals, however, there is no current examples of plasma membrane transport proteins that involve processing by HECT-type E3 ligases ( Mulet et al, 2013 ). However, it is probable, given the example of the Apollon, that there may be E2 enzymes involved in processing these membrane transporters without the need for a separate E3.…”

Section: Nutrients and Ubiquitinmentioning

confidence: 99%

Ubiquitination in plant nutrient utilization

Yates

Sadanandom

2013

Front. Plant Sci.

View full text Add to dashboard Cite

Ubiquitin (Ub) is well-established as a major modifier of signaling in eukaryotes. However, the extent to which plants rely on Ub for regulating nutrient uptake is still in its infancy. The main characteristic of ubiquitination is the conjugation of Ub onto lysine residues of acceptor proteins. In most cases the targeted protein is rapidly degraded by the 26S proteasome, the major proteolysis machinery in eukaryotic cells. The Ub-proteasome system is responsible for removing most abnormal peptides and short-lived cellular regulators, which, in turn, control many processes. This allows cells to respond rapidly to intracellular signals and changing environmental conditions. This perspective will discuss how plants utilize Ub conjugation for sensing environmental nutrient levels. We will highlight recent advances in understanding how Ub aids nutrient homeostasis by affecting the trafficking of membrane bound transporters. Given the overrepresentation of genes encoding Ub-metabolizing enzymes in plants, intracellular signaling events regulated by Ub that lead to transcriptional responses due to nutrient starvation is an under explored area ripe for new discoveries. We provide new insight into how Ub based biochemical tools can be exploited to reveal new molecular components that affect nutrient signaling. The mechanistic nature of Ub signaling indicates that dominant form of any new molecular components can be readily generated and are likely shed new light on how plants cope with nutrient limiting conditions. Finally as part of future challenges in this research area we introduce the newly discovered roles of Ub-like proteins in nutrient homeostasis.

show abstract

“…For instance, Na + and K + share the same concentration (460 and 10 mM, respectively) both in sea water and in the extracellular environment of mammalian blood cell, whereas their respective concentrations are roughly reversed if one looks inside the cell membrane. 39,40 On the other hand, they are key ingredients in a wealth of technological applications a) Electronic mail: ikeda.takashi@jaea.go.jp from electrocatalysis 41 to solar cells. 42 One of the most fundamental phenomena of alkali cations in aquatic physicalchemistry is their structure making and breaking properties.…”

Section: Introductionmentioning

confidence: 99%

Role of van der Waals corrections in first principles simulations of alkali metal ions in aqueous solutions

Ikeda

Boero

2015

The Journal of Chemical Physics

View full text Add to dashboard Cite

By resorting to a novel implementation of the first-principles-based van der Waals correction (vdWC) based on maximally localized Wannier functions (MLWFs), we inspect its performance and assess its reliability for aqueous solutions of alkali metal ions. In our implementation of vdWCs, an efficient extrapolation scheme is introduced to allow for affordable first principles molecular dynamics avoiding the explicit recalculation of MLWFs at each step. We find that vdWCs, when added to the widely used revPBE gradient corrected functional, influence substantially both structural and dynamical properties of water molecules, with particular emphasis on the hydration shell of the alkali cations. These effects are more evident for strong structure-making and -breaking cationic species. Moreover, self-diffusion coefficients and reorientation correlation times of solvating water molecules change systematically, showing a trend in better agreement with experiments with respect to simulations neglecting the long-range dispersion contributions.

show abstract

Endocytic regulation of alkali metal transport proteins in mammals, yeast and plants

Cited by 16 publications

References 260 publications

Regulation of the Yeast Hxt6 Hexose Transporter by the Rod1 α-Arrestin, the Snf1 Protein Kinase, and the Bmh2 14-3-3 Protein

Regulation of the Yeast Hxt6 Hexose Transporter by the Rod1 α-Arrestin, the Snf1 Protein Kinase, and the Bmh2 14-3-3 Protein

Ubiquitination in plant nutrient utilization

Role of van der Waals corrections in first principles simulations of alkali metal ions in aqueous solutions

Contact Info

Product

Resources

About