Endocrine regulation of ovarian development in the autogenous mosquito, Aedes atropalpus

Masler, Edward P.; Fuchs, Morton S.; Sage, Becky A.; O’Connor, John D.

doi:10.1016/0016-6480(80)90151-3

Cited by 43 publications

(25 citation statements)

References 21 publications

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“…This finding is very similar to previous studies with A. aegypti which showed that ILP/insulin signaling interact with amino acid sensing through the TOR pathway to activate ovary ECD production and VG synthesis Roy et al, 2007;Roy and Raikhel, 2011;Gulia Nuss et al, 2011). Prior studies show that G. atropalus ovaries predominantly produce ecdysone, which is converted to 20E in target tissues, and that 20E stimulates egg maturation in decapitated autogenous females (Masler et al, 1980;Birnbaum et al, 1984). Our study confirmed that 20E activates maturation not only of the first egg clutch but also of the second one in decapitated G. atropalpus females, and it alone stimulates VG synthesis in isolated G. atropalpus fat body.…”

Section: Discussionsupporting

confidence: 90%

“…Genetic analysis indicates that autogeny is monofactoral and not sex linked (O'Meara and Craig, 1969;Gwadz, 1970;O'Meara and Krasnick, 1970;O'Meara, 1972;Masler et al, 1981). Prior studies also clearly establish that maturation of a first clutch depends on the release of neurohormones from the brain, which stimulates ECD production by the ovaries, mobilization of teneral nutrient reserves in the fat body, and VG production (Van Handel, 1976;Fuchs et al, 1980;Masler et al, 1980;Masler et al, 1981;Masler et al, 1983;Birnbaum et al, 1984;Kelly et al, 1981;Kelly et al, 1984;Ma et al, 1984). The central question of this study was whether OEH and ILPs, which regulate egg maturation in A. aegypti, also regulate egg production in G. atropalpus.…”

Section: Discussionmentioning

confidence: 99%

“…Prior studies indicate that ECD production by the ovaries and yolk uptake by oocytes rise and fall within 10-50h post-eclosion (PE) followed by oviposition at 60-72h PE Masler et al, 1980;Masler et al, 1981;Birnbaum et al, 1984;Kelly et al, 1981;Kelly et al, 1984). Decapitation of G. atropalpus females immediately after eclosion blocks yolk uptake into oocytes, while injection of crude head extracts from A. aegypti and 20-hydroxyecdysone (20E) partially rescue egg maturation.…”

Section: Introductionmentioning

confidence: 99%

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Ovary ecdysteroidogenic hormone activates egg maturation in the mosquito, Georgecraigius atropalpus, after adult eclosion or a blood meal

Gulia-Nuss

Eum

Strand

et al. 2012

Journal of Experimental Biology

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SUMMARYThe rockpool mosquito, Georgecraigius atropalpus, is a facultatively autogenous species that produces its first egg clutch without a blood meal shortly after emergence. Several days after depositing this clutch, females must take a blood meal to produce a second egg clutch. Decapitation of females shortly after emergence or blood ingestion prevents egg maturation. Here, we report that a single injected dose of the neuropeptide ovary ecdysteroidogenic hormone (OEH) fully restored egg maturation in decapitated females in both circumstances. This neuropeptide and two insulin-like peptides (ILPs) are potent gonadotropins in the related yellow fever mosquito, Aedes aegypti. ILP3 was marginally restorative in decapitated G. atropalpus, and ILP4 had no effect. Egg maturation in non-and blood-fed G. atropalpus was dependent on the enzymatic mobilization of amino acids from stored protein or the blood meal for yolk protein (vitellogenin, VG) synthesis and uptake by oocytes. We further show that OEH stimulates serine protease activity in the fat body of newly eclosed females or in the midgut of blood-fed ones, and ecdysteroid hormone production by the ovaries of both females. In contrast, only 20-hydroxyecdysone stimulated VG synthesis in the fat body of non-and blood-fed females. Using RNA interference to knock down expression of the insulin receptor, we found that OEH still fully restored autogenous egg maturation. In summary, our results identify OEH as a primary regulator of egg maturation in both autogenous and blood-fed G. atropalpus females and suggest the shift from blood meal-dependent to blood meal-independent release of OEH is a key factor in the evolution of autogeny in this species.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ovary ecdysteroidogenic hormone activates egg maturation in the mosquito, Georgecraigius atropalpus, after adult eclosion or a blood meal

Gulia-Nuss

Eum

Strand

et al. 2012

Journal of Experimental Biology

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“…atropalpus are not presented, because pre-vitellogenic follicular arrest does not occur. Egg development is continuous in this autogenous species (Masler et al, 1980;A.T…”

Section: Pre-vitellogenic Follicle Development In Relation To Femalementioning

confidence: 96%

“…During this time, sugar ingestion also affects female reproduction (Foster, 1995) and so its influence on hormonal activity was also examined. Past studies have used ablation and implantation methods to determine the contribution of endocrine tissues Lea, 1963;Lea, 1964;Lea, 1970) and ecdysteroids (Kelly and Fuchs, 1980;Masler et al, 1980) to autogenous egg maturation. However, our study is the first to measure CA biosynthesis of JH and ovarian ecdysteroidogenesis during autogenous egg development in response to larval and adult nutrition.…”

Section: Introductionmentioning

confidence: 99%

Effects of larval nutrition on the endocrinology of mosquito egg development

Telang

Noriega

et al. 2006

Journal of Experimental Biology

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Juvenile hormone and 20‐hydroxyecdysone as primary and secondary stimuli of vitellogenesis in Aedes aegypti

Borovsky

Thomas

Carlson

et al. 1985

Arch Insect Biochem Physiol

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Female Aedes aegypti that were fed blood and immediately abdominally ligated did not deposit yolk. Injection of 20-hydroxyecdysone (1.5-5.0 ng) or topical application of juvenile hormone (JH:I analogue methoprene (25 pg) did not induce vitellogenesis in these abdomens. When blood-gorged ligated abdomens were treated with both hormones, however, vitellogenesis was stimulated in 60% of treated animals. Rocket immunoelectrophoresis indicated that vitellin concentration per follicle in treated animals was similar to that in intact controls. When ligated abdomens were first treated with methoprene and immediately injected with a crude head extract of egg development neurosecretory hormone, vitellogenin synthesis was induced at a rate similar to that in blood-fed controls. blethoprene at this concentration (25 pg), did not cause an increase in whole-body ecdysteroid titers. Larger amounts of methoprene (1.65 ng) were needed to stimulate egg development and ecdysteroid production.Implantation of ecdysone-secreting ovaries into ligated abdomens did not stimulate vitellogenesis in the recipients. However, in recipients that were first treated with methoprene (25 pg), implantation of ecdysone-secreting ovaries resulted in normal egg development.These experiments indicate that the appearance of JH precedes 20-hydroxyecdysone in stimulating vitellogenesis following blood feeding in Ae. 76Borovsky et al Using high-pressure liquid chromatography, gas chromatography, and gas chromatography/mass spectrometry with chemical ionization, we isolated and identified JH Ill in a group of 2-to 10-h and 8-h blood-fed animals. Our data provide evidence that JH Ill (6.0 pg per female), which has been recently identified in larval and adult Ae. aegypti, is also present 8 h after a blood meal.

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Endocrine regulation of ovarian development in the autogenous mosquito, Aedes atropalpus

Cited by 43 publications

References 21 publications

Ovary ecdysteroidogenic hormone activates egg maturation in the mosquito, Georgecraigius atropalpus, after adult eclosion or a blood meal

Ovary ecdysteroidogenic hormone activates egg maturation in the mosquito, Georgecraigius atropalpus, after adult eclosion or a blood meal

Effects of larval nutrition on the endocrinology of mosquito egg development

Juvenile hormone and 20‐hydroxyecdysone as primary and secondary stimuli of vitellogenesis in Aedes aegypti

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