Endocrine cells share expression of N‐CAM with neurones

Langley, O.K.; Aletsee, M.C.; Gratzl, Manfred

doi:10.1016/0014-5793(87)80885-2

Cited by 34 publications

(32 citation statements)

References 25 publications

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“…The scarcity of junctional complexes, at least in our culture system, indicates that they are not the cause of reconstructed topographic affinity between hormone-producing cells. Some cell adhesion molecules have been identified in the rat anterior pituitary, including NCAM (Langley et al, 1987;Berardi et al, 1995) and PB-cadherin (Sugimoto et al, 1996). Spangler and Delidow (1998) observed P-and N-cadherin expression in PRL-producing rat pituitary 253-1 cells, and Heinrich et al (1999) reported that N-cadherin mediates cell aggregation in rat somatolactotropic GH 3 cells.…”

Section: Discussionmentioning

confidence: 99%

Rat anterior pituitary cells in vitro can partly reconstruct in vivo topographic affinities

et al. 2003

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Hormone-producing cells in the rat anterior pituitary gland are not randomly distributed; rather, there are specific topographic affinities among five cell types (Noda et al., Acta Histochem. Cytochem. 2001;34:313-319). In this study we reconstructed these affinities, at least partially, in primary monolayer culture. Pituitary cells collected from adult male rats were enzymatically dispersed and cultured for 72 hr at a density of 1 ϫ 10 5 cells/cm 2 . We double-immunostained cells using antibodies against hormones, and then used confocal laser microscopy to examine the ability of the cells to attach to each other. We also statistically analyzed the affinity of all combinations of the five types of hormone-producing cells. We observed clusters by electron microscopy to identify junctional complexes between the cells. Confocal laser microscopy indicated that the features and attachment patterns of hormoneproducing cells in vivo were similar to those in vitro. Statistical analyses revealed that the rates at which the five types of hormone-producing cells attached to growth hormone (GH)-, prolactin (PRL), and luteinizing hormone (LH)-producing cells were unequal, which suggests there are specific topographic affinities. The specific rates of adrenocorticotropic hormone (ACTH)-producing cell attachment to GH cells, LH to PRL cells, and PRL to LH cells were high, whereas that of PRL attachment to PRL cells was low. In addition, the rates correlated with the data from our previous in vivo study. Ultrastructural observations revealed few junctional complexes between hormone-producing cells. These results indicate that anterior pituitary hormone-producing cells can attach to specific types of cells by means of specific and/or nonspecific adhesion factors, and can reconstruct the topographic nature of the pituitary gland. Anat Rec Part A 272A: 548 -555, 2003.

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Section: Discussionmentioning

confidence: 99%

Rat anterior pituitary cells in vitro can partly reconstruct in vivo topographic affinities

et al. 2003

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“…3a). Thus these cells express the same NCAM isoform, which appeilrs to be the most characteristic NCAM isoform in rat endocrine tissues and rat endocrine tumor eelllines [6,7] and in these endoerine tissues. Lane 7 (Fig.…”

Section: Resultsmentioning

confidence: 86%

“…The recent observation of neural cell adhesion molecules (NCAMs) in endocrine cells [6,7] and the neuroendocrine nature of certain lung cancer types (cf. [1]) suggested that these surface molecules may be pres-296 ent in a significant fraction of lung tumors.…”

Section: Resultsmentioning

confidence: 99%

“…Recently a cell surface molecule, the neural cell adhesion molecule (NCAM), has been shown to be a characteristic component of rat endocrine cells and its [6,7]. In addition, while in central nervous tissues NCAM occurs as three isoforms of molecular masses 180, 140 and 120 kDa, the NCAM isoform most characteristic of rat endocrine tissues is that of 140 kDa (NCAM 140).…”

Section: Introductionmentioning

confidence: 99%

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NCAM: A surface marker for human small cell lung cancer cells

1991

Lung Cancer

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Immunocytochemical and immunochemical techniques were used to study the expression of the neural cell adhesion molecule (NCAM) by human lung cancer cell lines. Intense surface staining for NCAM was found at light and electron microscopic levels on small celliung cancer cells. The NCAM polypeptide of M r 140000 (NCAM 140) was detected by immunoblotting in all of 7 small celliung cancer celllines examined and in one out of two of the closely related large cell cancer cell lines: it was not detected in cell lines obtained from one patient with a mesothelioma, in two cases of adenocarcinoma, nor in two cases of squamous cell cancer. In contrast, neuron-specific enolase was found by immunoblotting in all the lung cancer celliines tested and synaptophysin in all but the adenocarcinoma celliines. These antigens were localized intracellularly. The specific expression ofNCAM 140 by human small and large celliung carcinomas suggests its potential as a diagnostic marker.Lung cancer; Neural cell adhesion molecule; Marker

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“…NCAMs were first found in the nervous system, but subsequently were also found in polypeptide hormoneand steroid-producing endocrine cells [10][11][12][13][14][15]. NCAMs allow the formation of cell clusters by auto-adhesion of neighboring NCAM-bearing cells.…”

Section: Introductionmentioning

confidence: 99%

Expression and alternative splicing of the neural cell adhesion molecule NCAM in human granulosa cells during luteinization

1994

FEBS Letters

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Freshly aspirated human granulosa cells from pre-ovulatory follicles and granulosa cells luteinized in culture possess the neural cell adhesion molecule (NCAM) of approximate molecular mass of 140,000 and NCAM mRNA as confirmed by Sl-nuclease protection assays and RT-PCR. Moreover, in the process of luteinization the NCAM isoform pattern is modified. Isoforms containing an insert of 10 amino acids (termed VASE) in the extracellular domain of NCAM were supplemented by alternatively spliced isoforms without this insert. NCAM immunoreactivity, at light and electron microscope levels, was associated with the cell membrane of most granulosa cells which formed clusters. During time in culture an increasing subpopulation of granulosa cells, devoid of NCAM immunoreactivity, spread out and formed monolayers. This differential expression and the alternative splicing of NCAM during luteinization of granulosa cells raise the possibility that NCAM could be involved in folliculogenesis and the formation of the corpus luteum in the human.

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Endocrine cells share expression of N‐CAM with neurones

Cited by 34 publications

References 25 publications

Rat anterior pituitary cells in vitro can partly reconstruct in vivo topographic affinities

Rat anterior pituitary cells in vitro can partly reconstruct in vivo topographic affinities

NCAM: A surface marker for human small cell lung cancer cells

Expression and alternative splicing of the neural cell adhesion molecule NCAM in human granulosa cells during luteinization

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