Encyclopedia of Autographa californica nucleopolyhedrovirus genes

Cohen, David; Marek, Martin; Davies, Bryn G.; Vlak, Just M.; Oers, Monique M. van

doi:10.1007/s12250-009-3059-7

Cited by 32 publications

(39 citation statements)

References 288 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This cluster is markedly conserved in many NPVs (15), suggesting some common function(s) of the encoded proteins. Indeed, deletion of the ac53 gene in Ac-MNPV prevented assembly of nucleocapsids, and instead masses of electron-lucent tubular structures were produced (50).…”

Section: Discussionmentioning

confidence: 97%

Baculovirus VP1054 Is an Acquired Cellular PURα, a Nucleic Acid-Binding Protein Specific for GGN Repeats

Marek

Romier

Galibert

et al. 2013

J Virol

Self Cite

View full text Add to dashboard Cite

c Baculovirus VP1054 protein is a structural component of both of the virion types budded virus (BV) and occlusion-derived virus (ODV), but its exact role in virion morphogenesis is poorly defined. In this paper, we reveal sequence and functional similarity between the baculovirus protein VP1054 and the cellular purine-rich element binding protein PUR-alpha (PUR␣). The data strongly suggest that gene transfer has occurred from a host to an ancestral baculovirus. Deletion of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) vp1054 gene completely prevented viral cell-to-cell spread. Electron microscopy data showed that assembly of progeny nucleocapsids is dramatically reduced in the absence of VP1054. More precisely, VP1054 is required for proper viral DNA encapsidation, as deduced from the formation of numerous electron-lucent capsid-like tubules. Complementary searching identified the presence of genetic elements composed of repeated GGN trinucleotide motifs in baculovirus genomes, the target sequence for PUR␣ proteins. Interestingly, these GGN-rich sequences are disproportionally distributed in baculoviral genomes and mostly occurred in proximity to the gene for the major occlusion body protein polyhedrin. We further demonstrate that the VP1054 protein specifically recognizes these GGN-rich islands, which at the same time encode crucial proline-rich domains in p78/83, an essential gene adjacent to the polyhedrin gene in the AcMNPV genome. While some viruses, like human immunodeficiency virus type 1 (HIV-1) and human JC virus (JCV), utilize host PUR␣ protein, baculoviruses encode the PUR␣-like protein VP1054, which is crucial for viral progeny production.

show abstract

Section: Discussionmentioning

confidence: 97%

Baculovirus VP1054 Is an Acquired Cellular PURα, a Nucleic Acid-Binding Protein Specific for GGN Repeats

Marek

Romier

Galibert

et al. 2013

J Virol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Thus, a set of common genes (known as core genes) which seem to be crucial factors for some of the main biological functions (transcription of viral late genes, production of the virion structure, infection of gut cells, abrogation of the host metabolism, and establishment of the infection) have been identified by comparisons among all baculovirus genomes sequenced (12,15,36,50). To date, 33 genes are recognized as the set of encoding sequences shared by members of the family Baculoviridae (5,6,17,19,20,24,25,35,39,44,57). Core genes are commonly assumed to be orthologous genes as well.…”

mentioning

confidence: 99%

“…Taking the above-described situations into account, in the present work, we developed an algorithm to review or newly discover putative orthologous genes in addition to the 33 previously described orthologous collections for the Baculoviridae (5,6,17,19,20,24,25,35,39,44,57), using several additional bioinformatic approaches, such as PSI-Blast, BLASTP, tBlastN, and HaMStR (software that combines the Markov model with reciprocal best hits) (10); gene neighborhood conservation (38); and classical properties like protein size, the presence of transmembrane motifs, and additional protein analyses. This study provides new bioinformatic evidence to enrich the genetic knowledge of this important viral family.…”

mentioning

confidence: 99%

The ac53 , ac78 , ac101 , and ac103 Genes Are Newly Discovered Core Genes in the Family Baculoviridae

Garavaglia

Miele

Iserte

et al. 2012

J Virol

141

108

View full text Add to dashboard Cite

bThe family Baculoviridae is a large group of insect viruses containing circular double-stranded DNA genomes of 80 to 180 kbp, which have broad biotechnological applications. A key feature to understand and manipulate them is the recognition of orthology. However, the differences in gene contents and evolutionary distances among the known members of this family make it difficult to assign sequence orthology. In this study, the genome sequences of 58 baculoviruses were analyzed, with the aim to detect previously undescribed core genes because of their remote homology. A routine based on Multi PSI-Blast/tBlastN and Multi HaMStR allowed us to detect 31 of 33 accepted core genes and 4 orthologous sequences in the Baculoviridae which were not described previously. Our results show that the ac53, ac78, ac101 (p40), and ac103 (p48) genes have orthologs in all genomes and should be considered core genes. Accordingly, there are 37 orthologous genes in the family Baculoviridae.

show abstract

“…Every stage of the baculovirus infection process is associated with different protein groups (Cohen et al, 2009;Peng et al, 2010). The identification of more and more structural proteins is useful to identify which proteins take part in which stage of infection and to identify additional protein-protein interactions.…”

Section: Proteins Shared By Seven Identified Baculovirusesmentioning

confidence: 99%

Proteomic analysis of the occlusion-derived virus of Clostera anachoreta granulovirus

Zhang

Liang

et al. 2015

Journal of General Virology

View full text Add to dashboard Cite

To date, proteomic studies have been performed on occlusion-derived viruses (ODVs) from five members of the family Baculoviridae, genus Alphabaculovirus, but only a single member of the genus Betabaculovirus (Pieris rapae granulovirus). In this study, LC-MS/MS was used to analyse the ODV proteins of Clostera anachoreta granulovirus (ClanGV), another member of the genus Betabaculovirus. The results indicated that 73 proteins, including the products of 27 baculovirus core genes, were present in ClanGV ODVs. This is the largest number of ODV proteins identified in baculoviruses to date. To the best of our knowledge, 24 of these proteins were newly identified as ODV-associated proteins. Twelve of the proteins were shared by all seven of the other baculoviruses that have been analysed by proteomic techniques, including P49, PIF-2, ODV-EC43, P74, P6.9, P33, VP39, ODV-EC27, VP91, GP41, VLF-1 and VP1054. ClanGV shared between 20 and 36 ODV proteins with each of the other six baculoviruses that have been analysed by proteomics. Ten proteins were identified only as ODV components of ClanGV and PrGV: Clan22, Clan27, Clan69, Clan83, Clan84, Clan90, Clan116, Clan94, FGF-3 and ME53, the first seven of which were encoded by betabaculovirus-specific genes. These findings may provide novel insights into baculovirus structure as well as reveal similarities and differences between alphabaculoviruses and betabaculoviruses.

show abstract

Encyclopedia of Autographa californica nucleopolyhedrovirus genes

Cited by 32 publications

References 288 publications

Baculovirus VP1054 Is an Acquired Cellular PURα, a Nucleic Acid-Binding Protein Specific for GGN Repeats

Baculovirus VP1054 Is an Acquired Cellular PURα, a Nucleic Acid-Binding Protein Specific for GGN Repeats

The ac53 , ac78 , ac101 , and ac103 Genes Are Newly Discovered Core Genes in the Family Baculoviridae

Proteomic analysis of the occlusion-derived virus of Clostera anachoreta granulovirus

Contact Info

Product

Resources

About