The ac53 , ac78 , ac101 , and ac103 Genes Are Newly Discovered Core Genes in the Family Baculoviridae

Abstract

bThe family Baculoviridae is a large group of insect viruses containing circular double-stranded DNA genomes of 80 to 180 kbp, which have broad biotechnological applications. A key feature to understand and manipulate them is the recognition of orthology. However, the differences in gene contents and evolutionary distances among the known members of this family make it difficult to assign sequence orthology. In this study, the genome sequences of 58 baculoviruses were analyzed, with the aim to detect previously undescribed core genes because of their remote homology. A routine based on Multi PSI-Blast/tBlastN and Multi HaMStR allowed us to detect 31 of 33 accepted core genes and 4 orthologous sequences in the Baculoviridae which were not described previously. Our results show that the ac53, ac78, ac101 (p40), and ac103 (p48) genes have orthologs in all genomes and should be considered core genes. Accordingly, there are 37 orthologous genes in the family Baculoviridae.

Baculovirus FP25K Localization: Role of the Coiled-Coil Domain

“…FP25K orthologues are present in alpha-, beta-, and gammabaculoviruses and absent in deltabaculoviruses (30). To gain insight into functional differences in alphaand betabaculovirus FP25K proteins, Nakanishi et al examined phenotypic changes between recombinant Bombyx mori NPV (BmNPV) expressing FP25K from Autographa californica multiple NPV (AcMNPV), Spodoptera litura multiple NPV (SpltMNPV), or Xestia c-nigrum GV (XecnGV) (19).…”

Genômica, evolução e caracterização funcional de genes de baculovírus

“…1c). Interestingly, we found high number of SNPs in the genes p95, dna-helicase, and gp41 which are core genes in the family Baculoviridae (Garavaglia et al, 2012). However, the impact of this diversity in virus replication or pathogenicity is not clear.…”

“…As observed in ChocGV (Escasa et al, 2006), ErelGV lacks both gp37 and exon0, which was previously predicted for being shared among all Alpha and Betabaculovirus (Garavaglia et al, 2012). (Hayakawa et al, 1999) u/d -unpublished data…”

“…Conversely, previous work has showed that different BmNPV isolates had a high degree of sequence divergence in ORFs, which are not core genes, but otherwise might play an important role in the virus evolution (Xu et al, 2013). For instance, F protein, which is shared only among Alpha and Betabaculovirus, was found to present high level of SNPs as well (Garavaglia et al, 2012). The protein is believed to be a non-essential remnant protein in BmNPV-like viruses (Group I Alphabaculovirus) playing a role only on the virus pathogenicity (Lung et al, 2003).…”

O genoma de um baculovírus isolado de cadáveres de larvas de Lonomia obliqua (Lepidoptera: Saturniidae) : uma lagarta de interesse médico

“…A tabela 1 apresenta a função relacionada de todos os core genes de baculovírus. Desses genes, 37 são genes compartilhados que já foram identificados em todos os baculovírus sequenciados até o momento (Garavaglia et al 2012;Yuan 2011 Esses core genes foram provavelmente incorporados aos genomas desses vírus antes de processos de diversificação, uma vez que são encontrados em todos os quatro gêneros da família Baculoviridae, enquanto outros genes são encontrados em apenas alguns desses gêneros, sugerindo que essa incorporação ao genoma tenha ocorrido mais recentemente.…”