ElsevierLlaneras Estrada, F.; Sala, A.; Picó Marco, JA. (2012). Dynamic estimations of metabolic fluxes with constraint-based models and possibility theory. Journal of Process Control. 22(10):1946Control. 22(10): -1955Control. 22(10): . doi:10.1016Control. 22(10): /j.jprocont.2012
it is easy to take extracellular dynamics into account. This work explores the benefits of using possibility theory to get these dynamic predictions. It will be shown that the possibilistic methods (a) provide rich estimates for time-varying fluxes and metabolite concentrations, (b) account for uncertainty and data scarcity, and (c) give predictions relaxing the optimality assumption of FBA. On the other hand, these methods could serve as basis for monitoring and fault detection systems in industrial bioprocesses.
Highlights-Possibility theory is used to get dynamic estimates of fluxes and metabolites. -The approach handles measurements errors, uncertainty, and data scarcity. -Predictions can be obtained relaxing the assumption of cells evolved optimality.
KeywordsConstraint satisfaction problems, possibility theory, constraint-based model, metabolic flux analysis, flux balance analysis.