Encoded anisotropic particles for multiplexed bioanalysis

Cederquist, Kristin B.; Dean, Stacey L.; Keating, Christine D.

doi:10.1002/wnan.96

Cited by 48 publications

(46 citation statements)

References 130 publications

(290 reference statements)

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“…This is due to the unique and controllable physicochemical properties of micro- and nanoparticles, which allow them to integrate multiple functions such as biosensing1234, targeting5678 and drug delivery91011. Recently, a wide range of studies has focused on the fabrication and characterization of new micro- and nanoparticles for biomedical purposes.…”

mentioning

confidence: 99%

Surface modification of microparticles causes differential uptake responses in normal and tumoral human breast epithelial cells

Patiño

Soriano

Barrios

et al. 2015

Sci Rep

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The use of micro- and nanodevices as multifunctional systems for biomedical applications has experienced an exponential growth during the past decades. Although a large number of studies have focused on the design and fabrication of new micro- and nanosystems capable of developing multiple functions, a deeper understanding of their interaction with cells is required. In the present study, we evaluated the effect of different microparticle surfaces on their interaction with normal and tumoral human breast epithelial cell lines. For this, AlexaFluor488 IgG functionalized polystyrene microparticles (3 μm) were coated with Polyethyleneimine (PEI) at two different molecular weights, 25 and 750 kDa. The effect of microparticle surface properties on cytotoxicity, cellular uptake and endocytic pathways were assessed for both normal and tumoral cell lines. Results showed a differential response between the two cell lines regarding uptake efficiency and mechanisms of endocytosis, highlighting the potential role of microparticle surface tunning for specific cell targeting.

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confidence: 99%

Surface modification of microparticles causes differential uptake responses in normal and tumoral human breast epithelial cells

Patiño

Soriano

Barrios

et al. 2015

Sci Rep

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“…1 However, technical challenges in bead encoding have limited their practical application to date. 2 Existing encoding methods [1][2][3][4] generally fall into two categories: spatial encoding and spectral encoding. Spatial encoding schemes create graphical patterns or bar codes in the particle material in a variety of ways.…”

Section: Introductionmentioning

confidence: 99%

Programmable microfluidic synthesis of spectrally encoded microspheres

et al. 2012

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Spectrally encoded fluorescent beads are an attractive platform for assay miniaturization and multiplexing in the biological sciences. Here, we synthesize hydrophilic PEG-acrylate polymer beads encoded with lanthanide nanophosphors using a fully automated microfluidic synthesis device. These beads are encoded by including varying amounts of two lanthanide nanophosphors relative to a third reference nanophosphor to generate 24 distinct ratios. These codes differ by less than 3% from their target values and can be distinguished from each other with an error rate of <0.1%. The encoded bead synthesis strategy we have used is readily extensible to larger numbers of codes, potentially up to millions, providing a new platform technology for assay multiplexing.

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“…[ 60,61 ] After evaporating the solvent of the droplets, it was accessible to obtain composite polymer particles containing the fl uorescent elements. Despite having many commercial applications, fl uorescent dyes have the problems of photobleaching and red-tailed emission, which could affect the encoded information of the polymer particles.…”

Section: Fluorescent Particles From Single Emulsionsmentioning

confidence: 99%

Microfluidic Synthesis of Barcode Particles for Multiplex Assays

Zhao

Cheng

Shang

et al. 2014

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The increasing use of high-throughput assays in biomedical applications, including drug discovery and clinical diagnostics, demands effective strategies for multiplexing. One promising strategy is the use of barcode particles that encode information about their specific compositions and enable simple identification. Various encoding mechanisms, including spectroscopic, graphical, electronic, and physical encoding, have been proposed for the provision of sufficient identification codes for the barcode particles. These particles are synthesized in various ways. Microfluidics is an effective approach that has created exciting avenues of scientific research in barcode particle synthesis. The resultant particles have found important application in the detection of multiple biological species as they have properties of high flexibility, fast reaction times, less reagent consumption, and good repeatability. In this paper, research progress in the microfluidic synthesis of barcode particles for multiplex assays is discussed. After introducing the general developing strategies of the barcode particles, the focus is on studies of microfluidics, including their design, fabrication, and application in the generation of barcode particles. Applications of the achieved barcode particles in multiplex assays will be described and emphasized. The prospects for future development of these barcode particles are also presented.

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Encoded anisotropic particles for multiplexed bioanalysis

Cited by 48 publications

References 130 publications

Surface modification of microparticles causes differential uptake responses in normal and tumoral human breast epithelial cells

Surface modification of microparticles causes differential uptake responses in normal and tumoral human breast epithelial cells

Programmable microfluidic synthesis of spectrally encoded microspheres

Microfluidic Synthesis of Barcode Particles for Multiplex Assays

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