Encapsulation of moss buds: an efficient method for the in vitro conservation and regeneration of the endangered moss Splachnum ampullaceum

Mallón, Rubén; Barros, Paula; Luzardo, Asteria; Fernández-González, María

doi:10.1007/s11240-006-9176-1

Cited by 28 publications

(9 citation statements)

References 33 publications

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“…Further in vitro bryophyte collections are held by the Department of Gene Expression at Adam Mickiewicz University, Poland (personal communication Z. Sweykowska-Kulinska, AMU) and a number of research groups have worked on in vitro cultivation of bryophytes recently (e.g. Melosik and Såstad 2005;Mallon et al 2007) The International Moss Stock Centre, managed by the Department of Biotechnology at the Albert-Ludwigs University Freiburg maintains stocks of the model moss Physcomitrella patens and other closely related species (for more information see http://www.moss-stock-center. org/).…”

Section: European In Vitro Collections Of Bryophytes For Conservationmentioning

confidence: 99%

In vitro conservation of European bryophytes

Rowntree

Pressel

Ramsay

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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The use of in vitro techniques for conservation has been rising steadily since their inclusion in The Convention on Biological Diversity and The Global Strategy for Plant Conservation. Unfortunately, bryophytes are often overlooked in conservation initiatives, but they are important in a number of large-scale ecosystem processes, i.e. nutrient, water and carbon cycling. There is a long history of the use of tissue culture in cultivating bryophytes, and many species respond well to in vitro techniques. For 6 yr (2000)(2001)(2002)(2003)(2004)(2005)(2006), The Royal Botanic Gardens, Kew and the UK statutory conservation agencies supported a project for the ex situ conservation of bryophytes. Living and cryopreserved collections of UK threatened species were successfully established and the cryopreserved collection continues to be maintained. Other in vitro conservation collections are maintained over Europe, at botanic gardens, museums and by individual university researchers, but there is no coherent European collection of bryophytes for conservation, or standardisation of techniques. A major issue for many in vitro collections is the maintenance of within species genetic diversity. Such diversity is considered to be important, as it is the basis by which populations of species can adapt to new conditions and evolve. We are proposing to establish a European network for in vitro conservation of bryophytes. We envisage that this will include living collections, cryopreserved collections and spore collections. Conservation of genetic diversity would be a priority and the collections would provide a valuable resource for conservation initiatives and support research into rare and threatened species.

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Section: European In Vitro Collections Of Bryophytes For Conservationmentioning

confidence: 99%

In vitro conservation of European bryophytes

Rowntree

Pressel

Ramsay

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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“…by Maneerattanarungroj et al (2007). This increased survival could be due to encapsulation of the PLBs within calcium alginate beads that protect them from direct damage when subjected to desiccation (Mallon et al 2007;Srivastava et al 2009) and the avoidance of toxic cryoprotectants as the encapsulated PLBs are placed on sucrose-enriched medium. The sucrose molarities in the beads is further increased by desiccation which results in a glass transition during cooling in LN, preventing ice crystal formation (a cause of lethal damage to living cells) during exposure to ultra-low temperature (Engelmann and Takagi 2000).…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration

Khoddamzadeh

Sinniah

Lynch

et al. 2011

Plant Cell Tiss Organ Cult

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Protocorm-like bodies (PLBs) of Phalaenopsis bellina were successfully cryopreserved by the encapsulationdehydration approach. Various stages in obtaining successful cryopreservation using this method were optimized. Encapsulated PLBs precultured in half-strength MS medium supplemented with 0.75 M sucrose for 3 days exhibited the highest viability in terms of 2,3,5-triphenyltetrazoliumchloride (TTC) reduction. The amount of sucrose in the PLBs after incubation in different concentrations of sucrose for different periods of time determined by HPLC. The highest sucrose concentration was 7 mg/g of PLBs for the PLBs treated with 0.75 M sucrose for 3 days as compared to the control which had only 1 mg/g sucrose. After sucrose preculture, the PLBs were subjected to desiccation using one of two methods. Desiccation using silica gel was more efficient in reducing PLBs moisture content. After 6 h of desiccation, PLBs desiccated using laminar air flow had 43.5% moisture content while for those desiccated using silica gel had 32% moisture content. PLBs desiccated to different moisture contents were plunged into LN. After storage in LN the encapsulated PLBs were re-warmed. Two weeks after re-warming PLBs viability was determined by TTC reduction and re-growth assessed. Encapsulated PLBs precultured with 0.75 M sucrose for 3 days followed by desiccated using silica gel for 5 h resulting in a moisture content of 39% lead to the highest post re-warming viability in terms of TTC reduction (46.6% of control PLBs) and 30% re-growth.

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“…into plantlets in vitro or ex vitro conditions (AITKEN-CHRISTIE et al, 1995). Encapsulated technology using in vitro-grown propagules offers an efficient means for mass clonal propagation and conservation of elite or endangered plant species (MALLÓN et al, 2007). The main advantages using synthetic seed techniques are associated with the elimination of the final micropropagation stages (i.e.…”

Section: Introductionmentioning

confidence: 99%

Microshoots encapsulation and plant conversion of Musa sp. cv. 'Grand Naine'

et al. 2009

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Synthetic seed technologies are useful tools for the field delivery of in vitro derived plantlets. In the present study, different encapsulation procedures and their efficacy in the plantlet regeneration using microshoots of banana cv. 'Grand Naine' were evaluated. Two encapsulation systems were evaluated: i) single encapsulation in beads or droplet hardening method; and ii) double layer or hollow beads. The use of different compounds to enhance the capsule conservation and the conversion to plantlets was also evaluated. The conversion capacity was assessed in vitro on water-agar culture medium or in ex vitro conditions with Gerbox® boxes. The single encapsulation system showed 80% conversion. The capsules with MS saline formulation treated with 100mM KNO3 showed 76% conversion. Capsules with 1.5g L-1 activated charcoal, and 0.5g L-1 benomyl sucrose-free capsules showed 75% conversion. The encapsulated and non-encapsulated microshoots exhibited 100% germination in response to MS culture medium, and polyethylene glycol after 10 days of storage at 4°C. Sucrose-free capsules showed significantly higher germination (83.3%) than those sucrose-enriched capsules (56.7%). The ex vitro conversion of encapsulated microshoots was 20% in the Gerbox™. These results indicate the feasibility using synthetic seeds in the large-scale micropropagation of banana cv. 'Grand Naine'.

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Encapsulation of moss buds: an efficient method for the in vitro conservation and regeneration of the endangered moss Splachnum ampullaceum

Cited by 28 publications

References 33 publications

In vitro conservation of European bryophytes

In vitro conservation of European bryophytes

Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration

Microshoots encapsulation and plant conversion of Musa sp. cv. 'Grand Naine'

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