Encapsulation in alginate-polymers improves stability and allows controlled release of the UFV-AREG1 bacteriophage

Batalha, Laís Silva; Gontijo, Marco Túlio Pardini; Teixeira, Álvaro Vianna Novaes de Carvalho; Boggione, Delaine Meireles Gouvêa; Lopez, Maryoris Elisa Soto; Eller, Monique Renon; Mendonça, Regina Célia Santos

doi:10.1016/j.foodres.2020.109947

Cited by 29 publications

(24 citation statements)

References 72 publications

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“…In consequence, each sample exhibited non-Newtonian behavior. When the molecules align at high rates of deformation, apparent viscosity is reduced and pseudoplastic behavior occurs [ 29 ]. The flow behavior index for sodium alginate solution was 0.918, and Ma obtained the n = 0.898 for a 1% solution of sodium alginate [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

Biophysico-Chemical Properties of Alginate Oligomers Obtained by Acid and Oxidation Depolymerization

et al. 2021

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The aim of the study was to obtain alginate oligosaccharides by using two degradation methods of sodium alginate (SA): with hydrochloric acid (G—guluronate, M—mannuronate and G + M fractions) and hydrogen peroxide (HAS—hydrolyzed SA), in order to assess and compare their biological activity and physico-chemical properties, with an attempt to produce gels from the obtained hydrolysates. The efficiency of each method was determined in order to select the fastest and most efficient process. The ferric ion reducing antioxidant power (FRAP), the ability to scavenge DPPH free radicals, rheological properties, Fourier Transformed Spectroscopy (FTIR) and the microbiological test against Escherichia coli and Staphylococcus aureus were performed. In order to check the functional properties of the obtained oligosaccharides, the texture profile analysis was assessed. The hydrolysis yield of acid SA depolymerization was 28.1% and from hydrogen peroxide SA, depolymerization was 87%. The FTIR analysis confirmed the degradation process by both tested methods in the fingerprint region. The highest ferric reducing antioxidant power was noted for HSA (34.7 µg), and the highest hydroxyl radical scavenging activity was obtained by G fraction (346 µg/Trolox ml). The complete growth inhibition (OD = 0) of alginate hydrolysates was 1%. All tested samples presented pseudoplastic behavior, only HSA presented the ability to form gel.

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Section: Resultsmentioning

confidence: 99%

Biophysico-Chemical Properties of Alginate Oligomers Obtained by Acid and Oxidation Depolymerization

et al. 2021

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“…The loss of viability of t vB_kpnM_17-11 at pH = 1 prevents its oral administration because of the acidic conditions of the gastric environment ( Beasley et al., 2015 ). To overcome this drawback, vB_kpnM_17-11 can be protected by alginate and chitosan to avoid inactivation by gastric juices ( Silva Batalha et al., 2021 ). A limitation of vB_kpnM_17-11 is its narrow range, being able to lyse only 4 out of 96 tested strains of K. pneumoniae .…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of vB_kpnM_17-11, a Novel Phage Efficient Against Carbapenem-Resistant Klebsiella pneumoniae

Bai

Zhang

Liang

et al. 2022

Front. Cell. Infect. Microbiol.

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Phages and phage-encoded proteins exhibit promising prospects in the treatment of Carbapenem-Resistant Klebsiella pneumoniae (CRKP) infections. In this study, a novel Klebsiella pneumoniae phage vB_kpnM_17-11 was isolated and identified by using a CRKP host. vB_kpnM_17-11 has an icosahedral head and a retractable tail. The latent and exponential phases were 30 and 60 minutes, respectively; the burst size was 31.7 PFU/cell and the optimal MOI was 0.001. vB_kpnM_17-11 remained stable in a wide range of pH (4-8) and temperature (4-40°C). The genome of vB_kpnM_17-11 is 165,894 bp, double-stranded DNA (dsDNA), containing 275 Open Reading Frames (ORFs). It belongs to the family of Myoviridae, order Caudovirales, and has a close evolutionary relationship with Klebsiella phage PKO111. Sequence analysis showed that the 4530 bp orf022 of vB_kpnM_17-11 encodes a putative depolymerase. In vitro testing demonstrated that vB_kpnM_17-11 can decrease the number of K. pneumoniae by 105-fold. In a mouse model of infection, phage administration improved survival and reduced the number of K. pneumoniae in the abdominal cavity by 104-fold. In conclusion, vB_kpnM_17-11 showed excellent in vitro and in vivo performance against K. pneumoniae infection and constitutes a promising candidate for the development of phage therapy against CRKP.

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“…In order to protect bacteriophages from the mentioned factors, microencapsulation methods (i.e., entrapment of bacteriophages in an encapsulation system) using freeze drying, spray drying, ultrasonic vacuum spray drying, electrospraying, extrusion, emulsion, and supercritical techniques have been studied and reviewed intensively [21,[32][33][34][35]. As encapsulation materials, aginate solutions together with milk proteins such as alginate-caseinate [36,37] and alginate-whey protein [33,38] formulations were employed succesfully. In food processing, microencapsulation techniques are commonly utilized for sensitive food ingredients (e.g., proteins, oils, aroma, and probiotics) [34,39,40].…”

Section: Introductionmentioning

confidence: 99%

Microencapsulation of Bacteriophages for the Delivery to and Modulation of the Human Gut Microbiota through Milk and Cereal Products

et al. 2022

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There is a bidirectional interaction between the gut microbiota and human health status. Disturbance of the microbiota increases the risk of pathogen infections and other diseases. The use of bacteriophages as antibacterial therapy or prophylaxis is intended to counteract intestinal disorders. To deliver bacteriophages unharmed into the gut, they must be protected from acidic conditions in the stomach. Therefore, an encapsulation method based on in situ complexation of alginate (2%), calcium ions (0.5%), and milk proteins (1%) by spray drying was investigated. Powdered capsules with particle sizes of ~10 µm and bacteriophage K5 titers of ~108 plaque forming units (pfu) g−1 were obtained. They protected the bacteriophages from acid (pH 2.5) in the stomach for 2 h and released them within 30 min under intestinal conditions (in vitro). There was no loss of viability during storage over two months (4 °C). Instead of consuming bacteriophage capsules in pure form (i.e., as powder/tablets), they could be inserted into food matrices, as exemplary shown in this study using cereal cookies as a semi-solid food matrix. By consuming bacteriophages in combination with probiotic organisms (e.g., via yoghurt with cereal cookies), probiotics could directly repopulate the niches generated by bacteriophages and, thus, contribute to a healthier life.

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Encapsulation in alginate-polymers improves stability and allows controlled release of the UFV-AREG1 bacteriophage

Cited by 29 publications

References 72 publications

Biophysico-Chemical Properties of Alginate Oligomers Obtained by Acid and Oxidation Depolymerization

Biophysico-Chemical Properties of Alginate Oligomers Obtained by Acid and Oxidation Depolymerization

Isolation and Characterization of vB_kpnM_17-11, a Novel Phage Efficient Against Carbapenem-Resistant Klebsiella pneumoniae

Microencapsulation of Bacteriophages for the Delivery to and Modulation of the Human Gut Microbiota through Milk and Cereal Products

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