Encapsulated Enzyme: a Glucoamylase Copolymer System

Beck, Steven R.; Rase, Howard F.

doi:10.1021/i360048a002

Cited by 18 publications

(1 citation statement)

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“…Since adsorption on ionic carriers is effected through ionic bonding, it is likely that increased charge on the enzyme would lead to strong adsorption. This point has been confirmed by Solomon and Levin The method of entrapping glucoamylase in the lattice of polyacrylantide gels was used by many authors (8,64,74,123) as a technique of immobilization. The gels are prepared by polymerizing solutions containing monomers, acrylamide, and cross-linking agents, usually N,M'-methylene-bis-acrylamidet.…”

Section: V)mentioning

confidence: 72%

The effect of slow substrate diffusion on the activity, stability, and selectivity of immobilized enzymes: a theoretical and experimental study

Lee

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Section: V)mentioning

confidence: 72%

The effect of slow substrate diffusion on the activity, stability, and selectivity of immobilized enzymes: a theoretical and experimental study

Lee

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Polymernetzwerke zum Einschluß von Mikroorganismen

Klein

Hackel

Schara

et al. 1979

Angew. Makromol. Chem.

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ZUSAMMENFASSUNG:Es werden verschiedene Vernetzungsreaktionen beschrieben, die zur Herstellung von Biokatalysatoren durch EinschluD von Mikroorganismen dienen konnen. Durch geeignete Suspensionsverfahren konnen aus wasserloslichen odcr wasserunloslichen Monovinyl/Divinylmonomer-Systemen perlformige Copolymerisate hergestellt werden. Durch den Einschlul3 von feinen NaC1-Kristallen im Verlaufe des Hartungsprozesses gelingt es, Epoxid-H a n e herzustellen, die neben einer Makroporositat auch eine hohe Mikroporositat besitzen. Die Vernetzung von naturlichen halbsynthetischen oder synthetischen Polyelektrolyten mit mehrwertigen Kationen (,,ionotrope Gele") stellt ein sehr schonendes Einschlul3verfahren dar. Die Prazipitation von linearen, wasserunloslichen bzw. begrenzt wasserloslichen Polymeren fuhrt zu ,,Netzwerken" ohne spezifischen Bindungscharakter.Die Vorteile und Nachteile dcr verschiedenen Vernetzungsverfahren werden hinsichtlich der mechanischen (Druckfestigkeit) und chcmischen Eigenschaften (Toxizitiit, Stabilitat) der Produkte diskutiert. SUMMARY:Different network formation processes, applicable to obtain biocdtdlytic systems by entrapment of whole microbial cells are described. Properly chosen suspension techniques can be used to prepare copolymer beads from water soluble or water insoluble monovinyl divinylmonomer systems. Inclusion of small NaCl crystallites during the curing process leads to epoxy resins which possess a macroporous as well as a highly microporous structure. Crosslinking of natural, seminatural and synthetic polyelectrolytes with multivalent cations ("ionotropic gels") proves to be a very gentle entrapment procedure. Networks of nonspecific bonding character are obtained by precipitation of linear water insoluble or poorly water-soluble polymers.* Vortrag anlaDlich der gemeinsamen Tagung der Fachgruppe ,,Makromolekulare Chcmie" der Gesellschaft Deutscher Chemiker und des Fachausschusses ,,Physik der Hochpolymeren" der Deutschen Physikalischen Gescllschaft uber ,,Polymere Netzwcrke" in Bad Nauheim am 18. April 1978. 329J. Klein, U. Hackel, P. Schara und H. Eng Advantages and disadvantages of the different network formation processes are discussed with respect to the mechanical (compressibility) and chemical (toxicity, stability) properties of the products.

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Glucoamylase immobilized on acid‐hydrolyzed starch graft polyacrylonitrile

Slininger

Fanta

Abbott

1988

Biotech & Bioengineering

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A continuous stirred reactor fed maltose as substrate was used to show that acid hydrolyzed starch-g-polyacry-lonitrile and other polysaccharide graft copolymers can bind and retain significant quantities of active glucoamy-lase. Glucose productivities up to 2.7 g/g carrier/h were observed with the immobilized glucoamylase, and half-lives up to 1800 h were indicative of activity longevity. Factors influencing the immobilized enzyme activity and first-order decay rate included temperature, p(H), and carrier composition. In all cases, maltose was converted quantitatively to glucose with no evidence of reversion product formation.

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Encapsulated Enzyme: a Glucoamylase Copolymer System

Abstract: He received a B.S. in chemical engineering from Kansas State University {1969) and a Ph.D. from The University of Texas at Austin {1972). He served as a teaching assistant at the University of Texas and held an NDEA Title IV Fellowship.

Cited by 18 publications

References 8 publications

The effect of slow substrate diffusion on the activity, stability, and selectivity of immobilized enzymes: a theoretical and experimental study

The effect of slow substrate diffusion on the activity, stability, and selectivity of immobilized enzymes: a theoretical and experimental study

Polymernetzwerke zum Einschluß von Mikroorganismen

Glucoamylase immobilized on acid‐hydrolyzed starch graft polyacrylonitrile

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