Enantioseparations in open-tubular capillary electrochromatography: Recent advances and applications

Kapnissi‐Christodoulou, Constantina P.; Nicolaou, Athina G.; Stavrou, Ioannis J.

doi:10.1016/j.chroma.2016.07.039

Cited by 41 publications

(20 citation statements)

References 90 publications

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“…Among all compounds, which are currently analyzed in samples of considerable interest in the food field, chiral compounds have attracted the attention of several researchers. [7][8][9] Tsuda and colleaques first reported a number years ago the use of an octadecyl modified 30 μm i.d. Determination of amino acid enantiomers is an essential topic in food analysis, since the presence of D-enantiomer may indicate undesirable effects.…”

Section: Introductionmentioning

confidence: 99%

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Chiral separation and determination of amino acid enantiomers in fruit juice by open‐tubular nano liquid chromatography

Aydoğan

2018

Chirality

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A novel chiral porous-layer stationary phase was developed for use in open-tubular nano liquid chromatography. The stationary phase was prepared by an in-situ polymerization of 3-chloro-2-hydroxypropylmethacrylate (HPMA-Cl) and ethylene dimethacrylate (EDMA). The reactive chloro groups at the surface of the porous stationary phase were reacted with β-Cyclodextrin (β-CD). The resulting morphology was characterized by using scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FT-IR). The chromatographic performance of the column was evaluated by hydrophilic interaction chromatography (HILIC). Amino acids were used as test solutes. The running buffer conditions for the enantioseparation were found to be 85% acetonitrile (ACN):10%MeOH: 5% H O at 0.1% v/v trifluoro acetic acid (TFA) (flow rate: 800 nL/min). The enantioseparation provided high theoretical plate numbers up to 26 000 platesm . A good retention capacity within satisfactory retention times was also achieved. Real sample applicability of this column to the separation of amino acid enantiomers in fruit juice sample was demonstrated.

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Section: Introductionmentioning

confidence: 99%

“…5,6 The use of open-tubular columns has risen significantly in the microscale/nanoscale analytical separations. [7][8][9] Tsuda and colleaques first reported a number years ago the use of an octadecyl modified 30 μm i.d. capillary colummn.…”

Section: Introductionmentioning

confidence: 99%

Chiral separation and determination of amino acid enantiomers in fruit juice by open‐tubular nano liquid chromatography

Aydoğan

2018

Chirality

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“…Gas chromatography and supercritical fluid chromatography are other techniques that have a key application in the separation of chiral drugs . However, during the last decade, capillary electrophoresis (CE) has received a great attention within this field due to its high resolution, power, and selectivity …”

Section: Introductionmentioning

confidence: 99%

Enantiomeric separation of β‐blockers and tryptophan using heparin as stationary and pseudostationary phases in capillary electrophoresis

2018

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The separation methods of the enantiomers of two β-blockers and tryptophan were studied using capillary electrochromatography with heparin covalently as well as non-covalently, bonded onto the capillary inner wall as stationary phase and electrokinetic chromatography with heparin as pseudostationary phase. In the case of heparin, used as a stationary phase, the method was unable to resolve enantiomers in both cases β-blockers and tryptophan. On the other hand, when heparin was used as a pseudostationary phase, the resolution of the enantiomers was obtained only with 3-aminopropyltriethoxysilane which were immobilised onto the inner phase of the capillary. The results of this study let us infer that the electrostatic, hydrophobic, and steric interactions were involved in the separation mechanisms. The separation was achieved in less than 10 minutes under the optimized conditions: 30 mM phosphate buffer (pH 2.5) with the adding of 15 mg/mL of heparin at 15°C and 10 kV. The usefulness of heparin as a chiral selector both in electrokinetic chromatography using 3-aminopropyltriethoxysilane attached to the capillary was demonstrated for the first time. The developed method was powerful, sensitive, and fast, and it could be considered an important alternative to conventional methods used for chiral separation.

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“…4,5 In the agricultural field, the ineffective configuration of chiral pesticides not only does not have the effect of weeding but also may cause environmental pollution and endanger human health. [13][14][15][16] In CEC, the column is the most critical component. 6,7 Therefore, it is necessary for enantioseparation in the field of pharmaceutical and agricultural science.…”

mentioning

confidence: 99%

“…In recent years, enantioseparations using CEC attract more and more attention because of the short analysis time and low sample and solvent consumption. [13][14][15][16] In CEC, the column is the most critical component. Depending on fabricating techniques, three types of columns have been employed including packed columns, monolithic columns, and OT columns.…”

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confidence: 99%

Preparation and characterization of a new open‐tubular capillary column for enantioseparation by capillary electrochromatography

Tang

Qin

et al. 2019

Chirality

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In order to use the enantioseparation capability of cationic cyclodextrin and to combine the advantages of capillary electrochromatography (CEC) with opentubular (OT) column, in this study, a new OT-CEC, coated with cationic cyclodextrin (1-allylimidazolium-β-cyclodextrin [AI-β-CD]) as chiral stationary phase (CSP), was prepared and applied for enantioseparation. Synthesized AI-β-CD was characterized by infrared (IR) spectrometry and mass spectrometry (MS). The preparation conditions for the AI-β-CD-coated column were optimized with the orthogonal experiment design L 9 (3 4 ). The column prepared was characterized by scanning electron microscopy (SEM) and elemental analysis (EA). The results showed that the thickness of stationary phase in the inner surface of the AI-β-CD-coated columns was about 0.2 to 0.5 μm. The AI-β-CD content in stationary phase based on the EA was approximately 2.77 mmol·m −2 . The AI-β-CD-coated columns could separate all 14 chiral compounds (histidine, lysine, arginine, glutamate, aspartic acid, cysteine, serine, valine, isoleucine, phenylalanine, salbutamol, atenolol, ibuprofen, and napropamide) successfully in the study and exhibit excellent reproducibility and stability. We propose that the column, coated with AI-β-CD, has a great potential for enantioseparation in OT-CEC.

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Enantioseparations in open-tubular capillary electrochromatography: Recent advances and applications

Cited by 41 publications

References 90 publications

Chiral separation and determination of amino acid enantiomers in fruit juice by open‐tubular nano liquid chromatography

Chiral separation and determination of amino acid enantiomers in fruit juice by open‐tubular nano liquid chromatography

Enantiomeric separation of β‐blockers and tryptophan using heparin as stationary and pseudostationary phases in capillary electrophoresis

Preparation and characterization of a new open‐tubular capillary column for enantioseparation by capillary electrochromatography

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