Enantioseparation of hydroxyeicosatetraenoic acids by hydroxypropyl‐γ‐cyclodextrin‐modified micellar electrokinetic chromatography

Kodama, Shuji; Nakajima, Shuro; Ozaki, Hiromichi; Takemoto, Ryota; Itabashi, Yutaka; Kuksis, A.

doi:10.1002/elps.201600213

Cited by 23 publications

(12 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This method provided a simultaneous and complete separation of enantiomeric and regioisomeric 8‐, 11‐, 12‐, and 15‐HETEs, which is difficult to obtain using chiral‐phase HPLC (Fig. ) .…”

Section: Free Fatty Acidsmentioning

confidence: 99%

See 1 more Smart Citation

A digest of capillary electrophoretic methods applied to lipid analyzes

Poinsot

Meang

et al. 2018

Electrophoresis

View full text Add to dashboard Cite

Lipids are naturally occurring organic compounds that can be classified into a number of types based on their solubility in nonpolar organic solvents, and are generally insoluble in water. The great structural variety of these various types of lipids has led them to be components of many different biological substances such as oils, waxes, cellular membranes, tissues and biological fluids. The use of capillary electrophoresis (CE) for the study of lipids during the past 30 years has been relatively rare when compared to its use for other classes of biomolecules, primarily due to their insolubility in water. However, a number of interesting studies have been conducted, and as part of this review, we will present the different approaches that were used, which mainly consist of micellar kinetic chromatography and non-aqueous CE. The main advantages of the use of these techniques compared to GC is the very simple sample preparation that is required and, compared to LC, the very robust and quick separations that can be obtained. In this review, we present the various methods that have been reported in the literature that have been used for the study of fatty acids, phospholipids, glycerides, eicosanoids and sterols, with the inclusion of various tables presenting descriptions of the CE methods used as well as the methods of detection, including UV absorbance, fluorescence, mass spectrometry, and conductivity. This review aims to demonstrate that CE can be easily used for the analysis of lipids.

show abstract

Section: Free Fatty Acidsmentioning

confidence: 99%

“…( A ) 20 mM, ( B ) 30 mM, and ( C ) 40 mM. 1 : 11( S )‐HETE; 2 : 11( R )‐HETE; 3 :12( S )‐HETE; 4 : 12( R )‐HETE; 5 : 15( S )‐HETE; 6 : 15( R )‐HETE; 7 : 8( S )‐HETE; and 8 : 8( R )‐HETE .…”

Section: Free Fatty Acidsunclassified

A digest of capillary electrophoretic methods applied to lipid analyzes

Poinsot

Meang

et al. 2018

Electrophoresis

View full text Add to dashboard Cite

show abstract

“… employed ionic liquid (IL)‐type surfactant as PSP for MEKC separation of six nucleosides and provided a potential alternative to hydrophilic interaction chromatography (HILIC) in the analysis of those highly hydrophilic analytes. Further, chiral separations of real samples could be obtained as well by 2‐hydroxypropyl‐β‐cyclodextrin (HP‐β‐CD) MEKC method . Typically, the coupling of MEKC with ESI‐MS is highly problematic due to the presence of nonvolatile surfactants, such as the commonly applied SDS, CTAB.…”

Section: Mode Of Separationmentioning

confidence: 99%

Recent advances in microscale separation

et al. 2017

View full text Add to dashboard Cite

Recent advances in microscale separationThis review focuses on the recent advances on microscale separation technology, particularly on the new development on column and detection technologies in electrophoretic and chromatographic separation modes (i.e., CE, CEC, cLC, etc.). Representative examples illustrate also applications on the small molecules, peptides, proteins, polysaccharides, chiral and so on. 246 relevant articles are cited covering the literature published

show abstract

“…Electrokinetic chromatography (EKC) has been widely applied for enantioseparations having the following advantages, high resolution, efficient separation, cost effective and only requires low quantities of samples and [61,84] reagents. Enantiomeric separation occurs in EKC when a chiral selector such as β-CD is added to a pseudostationary phase [85].…”

Section: Mwcnts For Chiral Separation Of Pharmaceuticalsmentioning

confidence: 99%

Application of Carbon Nanotubes in Chiral and Achiral Separations of Pharmaceuticals, Biologics and Chemicals

Hemasa

Naumovski

Maher

et al. 2017

Preprint

View full text Add to dashboard Cite

Carbon nanotubes (CNTs) possess unique mechanical, physical, electrical and absorbability properties coupled with their nanometer dimensional scale that renders them extremely valuable for applications in many fields including nanotechnology and chromatographic separation. The aim of this review is to provide an updated overview about the applications of CNTs in chiral and achiral separations of pharmaceuticals, biologics and chemicals. Chiral SWCNTs and MWCNTs have been directly applied for the enantioseparation of pharmaceuticals and biologicals by using them as stationary or pseudostationary phases in chromatographic separation techniques such as high-performance liquid chromatography (HPLC), capillary electrophoresis (CE) and gas chromatography (GC). Achiral MWCNTs have been used for achiral separations as efficient sorbent objects in solid-phase extraction techniques of biochemicals and drugs. Achiral SWCNTs have been applied in achiral separation of biological samples. Achiral SWCNTs and MWCNTs have been also successfully used to separate achiral mixtures of pharmaceuticals and chemicals. Collectively, functionalized CNTs have been indirectly applied in separation science by enhancing the enantioseparation of different chiral selectors whereas, non-functionalized CNTs have shown efficient capabilities for chiral separations by using techniques such as encapsulation or immobilization in polymer monolithic columns.

show abstract

Enantioseparation of hydroxyeicosatetraenoic acids by hydroxypropyl‐γ‐cyclodextrin‐modified micellar electrokinetic chromatography

Cited by 23 publications

References 61 publications

A digest of capillary electrophoretic methods applied to lipid analyzes

A digest of capillary electrophoretic methods applied to lipid analyzes

Recent advances in microscale separation

Application of Carbon Nanotubes in Chiral and Achiral Separations of Pharmaceuticals, Biologics and Chemicals

Contact Info

Product

Resources

About