Enantioselective analysis of (R)- and (S)-atenolol in urine samples by a high-performance liquid chromatography column-switching setup

Lamprecht, Günther; Kraushofer, Tanja; Stoschitzky, Kurt; Lindner, Wolfgang

doi:10.1016/s0378-4347(00)00080-3

Cited by 64 publications

(36 citation statements)

References 9 publications

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“…Previous methods for the determination of atenolol in biological fluids have relied on HPLC with UV or fluorimetric detection (Czendlik et al, 1997;Giachetti et al, 1997;Guadrado et al, 2002;Lamprecht et al, 2000;Martins et al, 1997;Niopas et al, 2000;Ranta et al, 2002;Wu et al, 2002Wu et al, , 2003. The reported HPLC methods have mainly employed liquid-liquid extraction (Wu et al, 2002(Wu et al, , 2003Pires de Abreu et al, 2003) with a sensitivity of 10-20 ng/mL, based on 0.5-1.0 mL of sample size.…”

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confidence: 99%

Hydrophilic interaction liquid chromatographic tandem mass spectrometric determination of atenolol in human plasma

Francisco

et al. 2005

Biomedical Chromatography

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A hydrophilic interaction liquid chromatographic method with tandem mass spectrometry for the determination of atenolol, a beta-blocking agent, in human plasma has been developed and validated over the curve range of 10--2000 ng/mL. The assay was based on protein precipitation followed by evaporation of the extraction solvent, reconstitution with acetonitrile, and chromatography on an Hypersil silica column (50 x 4.6 mm) using a low aqueous--high organic mobile phase. The mobile phase consists of 85% acetonitrile, 15% water, 0.5% acetic acid and 0.04% trifluoroacetic acid and runs isocratically at a flow rate of 2.0 mL/min. The column ef fluent was split so that 50% of it was transferred into the LC-MS/MS interface operated in positive electrospray ionization mode. The chromatographic run time was 2.0 min per injection. Atenolol and the internal standard, atenolol-d(7), showed a retention time of 1.0 min. The inter-day and intra-day precision and accuracy of the quality control samples were <5.3% relative standard deviation and <8.0% relative error, respectively. To explore the application of the current method for the analysis of other beta-blocking agents, propranolol and metoprolol were tested under the same chromatographic conditions with retention times of 0.68 and 0.75 min, respectively. The present method could be used for therapeutic drug monitoring, pharmacokinetic and drug--drug interaction studies of beta-blocking agents.

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confidence: 99%

Hydrophilic interaction liquid chromatographic tandem mass spectrometric determination of atenolol in human plasma

Francisco

et al. 2005

Biomedical Chromatography

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“…[2] The methods of analysis of the bulk drugs are officially in the British Pharmacopoeia a potentiometric titration method for both drugs. [3] Several methods have been reported for the analysis of atenolol, including UV spectrophotometry, [4,5] colorimetry, [6] fluorimetry, [7] 1 HNMR, [8] IR, [9] voltammetry, [10,11] potentiometry, [12] TLC, [13,14] HPTLC/ UV detection, [15,16] GC/MS, [17] HPGC/MS, [18] HPLC/UV detection, [19,20] HPLC-tandem mass spectrometry (HPLC/MS/MS), [21,22] ultraperformance liquid chromatography with tandem mass spectrometric (UPLC/MS/MS), [23] HPLC-fluorescence detection, [24] capillary liquid chromatography (CLC) and pressurized capillary electrochromatography (pCEC), [25] CZE, [26,27] miceller electrokinetic chromatography (MEKC) [28] and FIA-UV detection. [29] Reviewing the literature revealed that up to the present time, nothing has been published concerning the chemiluminescence determination of atenolol.…”

Section: Introductionmentioning

confidence: 99%

Determination of enalapril maleate and atenolol in their pharmaceutical products and in biological fluids by flow‐injection chemiluminescence

Alarfaj¹,

Al-Abdulkareem

Aly³

2009

Luminescence

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A chemiluminescent method using flow injection (FI) was investigated for rapid and sensitive determination of enalapril maleate and atenolol, which are used in the treatment of hypertension. The method is based on the sensitizing effect of these drugs on the Ce(IV)-sulfite reaction. The different experimental parameters affecting the chemiluminescence (CL) intensity were carefully studied and incorporated into the procedure. The method permitted the determination of 0.01-3.0 microg mL(-1) of enalapril maleate in bulk form with correlation coefficient r = 0.99993, lower limit of detection (LOD) 0.0025 microg mL(-1) (S/N = 2) and lower limit of quantitation (LOQ) 0.01 microg mL(-1). The linearity range of atenolol in bulk form was 0.01-2.0 microg mL(-1) (r = 0.99989) with LOD of 0.0003 microg mL(-1) (S/N = 2) and LOQ of 0.01 microg mL(-1). In biological fluids the linearity range of enalapril maleate was 0.1-2.0 microg mL(-1) in both urine and serum, and for atenolol the linearity range was 0.1-1.0 microg mL(-1) in both urine and serum. The method was also applied to the determination of the drugs in their pharmaceutical preparations.

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“…Para tanto, foram utilizadas duas fases estacionárias quirais uma derivada de celulose (Chiralcel OD ® ) (Figura 2) e a outra derivada de dinitrobenzoila (α-Burke 2 ® ) (Figura 3). Recentemente, alguns pesquisadores empregaram cromatografia líquida de alta eficiência com colunas dos tipos Chiralcel OD ® (Balmer et al, 1991;Ching et al, 1989;Ekelund et al, 1995;Herring, Bastian, Lalonde, 1991;Krstulovic, 1988;Rutledge, Garrick, 1989;Straka et al, 1990;Zhang, Stewart, Ujhelyi, 1995), β-ciclodextrina (Ekelund et al, 1995;He et al, 1993;Ward, Armstrong, 1988) e α-1-ácido glicoproteína (AGP) (Berthault, Kintz, Mangin, 1997;Ceccato, Hubert, Crommen, 1997;Enquist, Hermansson, 1990;Hermansson, Von Bahr, 1982;Svensson, Vessman, Karlsson, 1999) e antibiótico macrocíclico (teicoplanina -Chirobiotic T ® ) (Lamprecht et al, 2000;Mistry, Leslie, Eddington, 2001) para a determinação enantiomérica dos FIGURA 1 -Estrutura química dos β-bloqueadores. β-bloqueadores.…”

Section: Introductionunclassified

Cromatografia líquida com fase quiral aplicada na separação enantiomérica de fármacos cardiovasculares

Singh

Kedor-Hackmann

Santoro

2006

Rev. Bras. Cienc. Farm.

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Enantioselective analysis of (R)- and (S)-atenolol in urine samples by a high-performance liquid chromatography column-switching setup

Cited by 64 publications

References 9 publications

Hydrophilic interaction liquid chromatographic tandem mass spectrometric determination of atenolol in human plasma

Hydrophilic interaction liquid chromatographic tandem mass spectrometric determination of atenolol in human plasma

Determination of enalapril maleate and atenolol in their pharmaceutical products and in biological fluids by flow‐injection chemiluminescence

Cromatografia líquida com fase quiral aplicada na separação enantiomérica de fármacos cardiovasculares

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