Enantiomeric separation of basic compounds using heptakis(2,3‐di‐O‐methyl‐6‐O‐sulfo)‐β‐cyclodextrin in combination with potassium camphorsulfonate in nonaqueous capillary electrophoresis: Optimization by means of an experimental design

Abstract

The enantiomeric separation of a series of basic pharmaceuticals (beta-blockers, local anesthetics, sympathomimetics) has been investigated in nonaqueous capillary electrophoresis (NACE) systems using heptakis(2,3-di-O-methyl-6-O-sulfo)-beta-cyclodextrin (HDMS-beta-CD) in combination with potassium camphorsulfonate (camphorSO3-). For this purpose, a face-centered central composite design with 11 experimental points was applied. The effect of the concentrations of HDMS-beta-CD and camphorSO3- on enantioresolution was statistically evaluated and depended largely on the considered analyte. The presence of camphorSO3- was found to be particularly useful for the enantioseparation of compounds with high affinity for the anionic CD. CamphorSO3- seems to act as a competitor, reducing the affinity for the CD, probably by ion-pair formation with these analytes. For compounds with lower affinity for HDMS-beta-CD, the combination of camphorSO3- and the CD appeared to have a favorable effect on enantioresolution only if the optimal CD concentration could be reached. On the other hand, for compounds characterized by a very low affinity for the anionic CD, the association of camphorSO3- and HDMS-beta-CD is always unfavorable. Finally, experimental conditions were selected by means of the multivariate approach in order to obtain the highest resolution (Rs) value for each studied compound.

Optimization of micellar electrokinetic capillary chromatography method using central composite design for the analysis of components in Yangwei granule

“…Although several papers using CCD to optimize the separation conditions of CE have been published, many problems still exist. First, in many papers, the optimum separation conditions were obtained from the response surface (Servais et al, 2004). From the CCD results, regression equations reflecting the relationship between the responses (Y) and the factors (X) are obtained.…”

Synthesis of a single-isomer sulfated β-cyclodextrin carrying nonidentical substituents at all of the C2, C3, and C6 positions and its use for the electrophoretic separation of enantiomers in acidic aqueous and methanolic background electrolytes, Part 2: Heptakis(2-O-methyl-6-O-sulfo) cyclomaltoheptaose

“…[24][25][26][27][28][29][30][31]). Typically, SISCDs that carry OH groups at both the C2 and C3 positions and sulfo groups at the C6 positions are not sufficiently soluble in methanol.…”

CE‐MS method development for peptides analysis, especially hepcidin, an iron metabolism marker

“…The coefficients of the model and their statistical significance are listed in Table 3. The p-value is the probability of getting a result as extreme or more extreme than the one observed if the proposed null hypothesis is correct [25]. Considering the application (analysis of peptides in CE-ESI-MS), a p-valueo0.1 was considered as significant.…”