A biodegradable co-polymer, Poly (butylene succinate-co-butyene terephthalate) (PBST) was fabricated into nanofibers by electrospinning. A 4-factor 3-level L 9(34) orthogonal experimental design was employed to examine the effects of process parameters on morphologies of PBST nanofibers. The results showed that the weight concentration = 13wt%, the applied voltage = 20kV, the spinning speed = 0.5ml/h, the electrode distance = 10cm and the diameter of needle = 0.7mm were identified as the optimum conditions.