Enamel-like tissue regeneration by using biomimetic enamel matrix proteins

Fang, Zehui; Guo, Mengxi; Zhou, Qingli; Li, Quanli; Wong, Hai Ming; Cao, Chris Ying

doi:10.1016/j.ijbiomac.2021.06.028

Cited by 20 publications

(14 citation statements)

References 56 publications

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“…Several approaches have been used and tested to regenerate teeth [ 67 ]; these approaches have focused on targeted regeneration of individual tooth components such as dental pulp and dentin [for reviews see 68 – 70 ; for functional studies see 70 – 73 ], cementum [for reviews see 74 , 75 ; for functional studies see 76 – 78 ], periodontium [for reviews see 79 , 80 ; for functional studies see 81 – 83 ], and enamel [for reviews see 84 ; for functional studies see 85 – 88 ]. However, combining these strategies does not guarantee the successful regeneration of a viable tooth.…”

Section: Overview Of Different Types Of Dental Stem Cellsmentioning

confidence: 99%

Hypes and Hopes of Stem Cell Therapies in Dentistry: a Review

Baena

Casasco

Monti

2022

Stem Cell Rev and Rep

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One of the most exciting advances in life science research is the development of 3D cell culture systems to obtain complex structures called organoids and spheroids. These 3D cultures closely mimic in vivo conditions, where cells can grow and interact with their surroundings. This allows us to better study the spatio-temporal dynamics of organogenesis and organ function. Furthermore, physiologically relevant organoids cultures can be used for basic research, medical research, and drug discovery. Although most of the research thus far focuses on the development of heart, liver, kidney, and brain organoids, to name a few, most recently, these structures were obtained using dental stem cells to study in vitro tooth regeneration. This review aims to present the most up-to-date research showing how dental stem cells can be grown on specific biomaterials to induce their differentiation in 3D. The possibility of combining engineering and biology principles to replicate and/or increase tissue function has been an emerging and exciting field in medicine. The use of this methodology in dentistry has already yielded many interesting results paving the way for the improvement of dental care and successful therapies. Graphical abstract

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Section: Overview Of Different Types Of Dental Stem Cellsmentioning

confidence: 99%

Hypes and Hopes of Stem Cell Therapies in Dentistry: a Review

Baena

Casasco

Monti

2022

Stem Cell Rev and Rep

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“…These calcium chelates could act as calcium ion reservoir to promote the remineralization process of enamel by preventing the high supersaturation of calcium, which might lead to the nucleation process to occurs too quickly which inhibits the crystals growth. 5,101,102 The hexagonal crystals of the fluorapatite are formed after the addition of fluoride ions to the solution and incorporation of the ions into the HAp lattice. However, the process takes several days, which restricts clinical applications of this method, and also, the chelating agent, HEDTA, is not safe to be consumed by the patient; thus, a device is required to be designed to separate the mineralization solution from the oral environment.…”

Section: Novel Trends and State Of The Art Approaches For Enamel Repa...mentioning

confidence: 99%

“…142 Biomimetic EMPs consisting of modified leucine-rich amelogenin peptide and non-amelogenin analog have shown the ability to induce the regeneration of the enamel crystals with prism- and interprism-like structures once applied on the surface of the etched enamel. 102…”

Section: Novel Trends and State Of The Art Approaches For Enamel Repa...mentioning

confidence: 99%

Novel trends, challenges and new perspectives for enamel repair and regeneration to treat dental defects

et al. 2022

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“…Tooth tissue engineering employs three main elements including dental stem cells, scaffolds, and growth factors to reconstruct new dental tissues that resemble the structure and function of the native tissue [ 5 , 7 , 8 ]. Dental enamel is developed through the amelogenesis process during which enamel crystals are grown in a gel-like organic matrix secreted by ameloblasts [ 9 ]. Hydrogels have been used as models for enamel mineralization due to their capacity to mimic the in vivo enamel matrix, and have shown to promote the regrowth of enamel crystals and to form enamel-like structures [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Self-Crosslinkable Oxidized Alginate-Carboxymethyl Chitosan Hydrogels as an Injectable Cell Carrier for In Vitro Dental Enamel Regeneration

Mohabatpour

Yazdanpanah

Papagerakis

et al. 2022

JFB

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Injectable hydrogels, as carriers, offer great potential to incorporate cells or growth factors for dental tissue regeneration. Notably, the development of injectable hydrogels with appropriate structures and properties has been a challenging task, leaving much to be desired in terms of cytocompatibility, antibacterial and self-healing properties, as well as the ability to support dental stem cell functions. This paper presents our study on the development of a novel self-cross-linkable hydrogel composed of oxidized alginate and carboxymethyl chitosan and its characterization as a cell carrier for dental enamel regeneration in vitro. Oxidized alginate was synthesized with 60% theoretical oxidation degree using periodate oxidation and characterized by Fourier Transform Infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, and Ultraviolet-visible absorption spectroscopy. Then, hydrogels were prepared at three varying weight ratios of oxidized alginate to carboxymethyl chitosan (4:1, 3:1, and 2:1) through Schiff base reactions, which was confirmed by Fourier Transform Infrared spectroscopy. The hydrogels were characterized in terms of gelation time, swelling ratio, structure, injectability, self-healing, antibacterial properties, and in vitro characterization for enamel regeneration. The results demonstrated that, among the three hydrogels examined, the one with the highest ratio of oxidized alginate (i.e., 4:1) had the fastest gelation time and the lowest swelling ability, and that all hydrogels were formed with highly porous structures and were able to be injected through a 20-gauge needle without clogging. The injected hydrogels could be rapidly reformed with the self-healing property. The hydrogels also showed antibacterial properties against two cariogenic bacteria: Streptococcus mutans and Streptococcus sobrinus. For in vitro enamel regeneration, a dental epithelial cell line, HAT-7, was examined, demonstrating a high cell viability in the hydrogels during injection. Furthermore, HAT-7 cells encapsulated in the hydrogels showed alkaline phosphatase production and mineral deposition, as well as maintaining their round morphology, after 14 days of in vitro culture. Taken together, this study has provided evidence that the oxidized alginate-carboxymethyl chitosan hydrogels could be used as an injectable cell carrier for dental enamel tissue engineering applications.

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Enamel-like tissue regeneration by using biomimetic enamel matrix proteins

Cited by 20 publications

References 56 publications

Hypes and Hopes of Stem Cell Therapies in Dentistry: a Review

Hypes and Hopes of Stem Cell Therapies in Dentistry: a Review

Novel trends, challenges and new perspectives for enamel repair and regeneration to treat dental defects

Self-Crosslinkable Oxidized Alginate-Carboxymethyl Chitosan Hydrogels as an Injectable Cell Carrier for In Vitro Dental Enamel Regeneration

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