Enabling the inclusion of non-hydrolysed sulfated long term anabolic steroid metabolites in a screening for doping substances by means of gas chromatography quadrupole time-of-flight mass spectrometry

Albertsdóttir, Aðalheiður Dóra; Gansbeke, Wim Van; Eenoo, Peter Van; Polet, Michaël

doi:10.1016/j.chroma.2021.462039

Cited by 11 publications

(9 citation statements)

References 34 publications

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“…The combined sample procedure has been previously published 22 . Briefly, 0.5 ml of urine was aliquoted to two separate tubes, labelled A and B for the combined non‐hydrolysed sulphate fraction and hydrolysed glucuronated fraction.…”

Section: Methodsmentioning

confidence: 99%

“…As such, the core structure of the steroid molecule remains intact, facilitating detection, characterisation and confirmation of the steroid by for example further fragmentation to obtain more compound‐specific ions. Recently, a new straightforward sample preparation procedure was published, consisting of the combination of two liquid–liquid extractions (LLE), that enabled the simultaneous detection of non‐hydrolysed sulphated steroids and the conventional steroid metabolites in the same GC–MS run 22 …”

Section: Introductionmentioning

confidence: 99%

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Detection time comparison of non‐hydrolysed sulphated metabolites of metenolone, mesterolone and 17α‐methyltestosterone analysed by four different mass spectrometric techniques

Albertsdóttir

Gansbeke

Eenoo

et al. 2023

Drug Testing and Analysis

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The frequent detection of anabolic androgenic steroids (AAS) indicates their popularity among rule-breaking athletes. The so called long-term metabolites play a crucial role in their detection, and non-hydrolysed sulphated metabolites have gained renewed interest, as research has demonstrated their extended detection time compared to the more conventional markers (e.g., for metenolone and mesterolone).

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection time comparison of non‐hydrolysed sulphated metabolites of metenolone, mesterolone and 17α‐methyltestosterone analysed by four different mass spectrometric techniques

Albertsdóttir

Gansbeke

Eenoo

et al. 2023

Drug Testing and Analysis

Self Cite

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“…In the measurement of anabolic androgenic steroids (AASs) analyzed with high frequency in previous years, the conjugated steroids are often used as target analytes for the steroids conventionally. In a previous report, sulfated metabolites were found to have a long detectable time [84]. Simultaneous analysis of the sulphated AASs and the other mandatory targets was proposed using full scan high resolution GClow energy electron ionization (LEEI)-MS, with the sample procedure consisting of two liquid-liquid extraction (LLE).…”

Section: Ms 41 Gc-msmentioning

confidence: 99%

Rapidity and Precision of Steroid Hormone Measurement

Osaka

2022

JCM

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Steroids are present in all animals and plants, from mammals to prokaryotes. In the medical field, steroids are commonly classified as glucocorticoids, mineralocorticoids, and gonadal steroid hormones. Monitoring of hormones is useful in clinical and research fields for the assessment of physiological changes associated with aging, disease risk, and the diagnostic and therapeutic effects of various diseases. Since the discovery and isolation of steroid hormones, measurement methods for steroid hormones in biological samples have advanced substantially. Although immunoassays (IAs) are widely used in daily practice, mass spectrometry (MS)-based methods have been reported to be more specific. Steroid hormone measurement based on MS is desirable in clinical practice; however, there are several drawbacks, including the purchase and maintenance costs of the MS instrument and the need for specialized training of technicians. In this review, we discuss IA- and MS-based methods currently in use and briefly present the history of steroid hormone measurement. In addition, we describe recent advances in IA- and MS-based methods and future applications and considerations.

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“…Testing comprehensively and sensitively for hundreds of target analytes remains mandatory in sports drug testing, and ITP performance characteristics are continuously optimized, as, for example, shown by Albertsdottir et al 42 Here, two urine sample extracts, one obtained by liquid‐liquid extraction (LLE), and one produced by enzymatic hydrolysis employing β‐glucuronidase and subsequent LLE were combined prior to trimethylsilylation and GC/EI/QTOF analysis. The instrument was equipped with a 15 m × 200 μm (inner diameter) capillary column with a film thickness of 0.11 μm, and EI was conducted using 18 eV followed by full scan mass spectrometry ( m/z 50–750).…”

Section: Anabolic Agentsmentioning

confidence: 99%

Annual banned‐substance review: Analytical approaches in human sports drug testing 2020/2021

Thevis

Kuuranne

Geyer

2021

Drug Testing and Analysis

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Most core areas of anti‐doping research exploit and rely on analytical chemistry, applied to studies aiming at further improving the test methods' analytical sensitivity, the assays' comprehensiveness, the interpretation of metabolic profiles and patterns, but also at facilitating the differentiation of natural/endogenous substances from structurally identical but synthetically derived compounds and comprehending the athlete's exposome. Further, a continuously growing number of advantages of complementary matrices such as dried blood spots have been identified and transferred from research to sports drug testing routine applications, with an overall gain of valuable additions to the anti‐doping field. In this edition of the annual banned‐substance review, literature on recent developments in anti‐doping published between October 2020 and September 2021 is summarized and discussed, particularly focusing on human doping controls and potential applications of new testing strategies to substances and methods of doping specified in the World Anti‐Doping Agency's 2021 Prohibited List.

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Enabling the inclusion of non-hydrolysed sulfated long term anabolic steroid metabolites in a screening for doping substances by means of gas chromatography quadrupole time-of-flight mass spectrometry

Cited by 11 publications

References 34 publications

Detection time comparison of non‐hydrolysed sulphated metabolites of metenolone, mesterolone and 17α‐methyltestosterone analysed by four different mass spectrometric techniques

Detection time comparison of non‐hydrolysed sulphated metabolites of metenolone, mesterolone and 17α‐methyltestosterone analysed by four different mass spectrometric techniques

Rapidity and Precision of Steroid Hormone Measurement

Annual banned‐substance review: Analytical approaches in human sports drug testing 2020/2021

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